Analysis of intercomponent energy transfer in the interaction of oscillating-grid turbulence with an impermeable boundary

New experimental results are presented that investigate the nature of the intercomponent energy transfer that occurs in the interaction between oscillating-grid turbulence and a solid impermeable boundary, using instantaneous velocity measurements obtained from two-dimensional particle imaging velocimetry (PIV). Estimates of the pressure-strain correlation term of the transport equation of the Reynolds stress tensor, which represents intercomponent energy transfer, are obtained using the PIV data from a balance of the remaining terms of the transport equation. The influence of the pressure-strain correlation term on the flow is examined by computing the energy spectra and conditional turbulent statistics associated with events in which intercomponent energy transfer is thought to be concentrated. Data reported here is in support of viscous and `return-to-isotropy' mechanisms governing the intercomponent energy transfer previously proposed, respectively, by Perot & Moin [B. Perot and P. Moin, J. Fluid Mech., 295, 199-227 (1995).] and Walker et al. [D. T. Walker, R. I. Leighton and L. O. Garza-Rios, J. Fluid Mech., 320, 19-51 (1996)]. However, the data reported also indicate the presence of a weak net intercomponent energy transfer from the boundary-normal velocity component to the boundary-tangential velocity components over a thin region outside the viscous sublayer which is not captured within existing models of intercomponent energy transfer at the boundary

Opioid prescribing for acute postoperative pain : an overview of systematic reviews related to two consensus statements relevant at patient, prescriber, system and public health levels

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Experimental study of oscillating-grid turbulence interacting with a solid boundary

© 2017 Cambridge University Press. The interaction between oscillating-grid turbulence and a solid, impermeable boundary (positioned below, and aligned parallel to, the grid) is studied experimentally. Instantaneous velocity measurements, obtained using two-dimensional particle imaging velocimetry in the vertical plane through the centre of the (horizontal) grid, are used to study the effect of the boundary on the root-mean-square velocity components, the vertical flux of turbulent kinetic energy (TKE) and the terms in the Reynolds stress transport equation. Identified as a critical aspect of the interaction is the blocking of a vertical flux of TKE across the boundary-affected region. Terms of the Reynolds stress transport equations show that the blocking of this energy flux acts to increase the boundary-tangential turbulent velocity component, relative to the far-field trend, but not the boundary-normal velocity component. The results are compared with previous studies of the interaction between zero-mean-shear turbulence and a solid boundary. In particular, the data reported here are in support of viscous and 'return-to-isotropy' mechanisms governing the intercomponent energy transfer previously proposed, respectively, by Perot and Moin (J. Fluid Mech., vol. 295, 1995, pp. 199-227) and Walker et al. (J. Fluid Mech., vol. 320, 1996, pp. 19-51), although we note that these mechanisms are not independent of the blocking of energy flux and draw parallels to the related model proposed by Magnaudet (J. Fluid Mech., vol. 484, 2003, pp. 167-196)

A method for reducing mean flow in oscillating‑grid turbulence

Oscillating-grid turbulence (OGT) is an experimental tool that has been widely used to study the role of turbulent fluctuations under conditions of small mean flow. We report experiments to investigate the structure of the turbulent flow produced by an oscillating grid, using velocity measurements obtained through the application of two-dimensional particle image velocimetry in the vertical plane through the centre of the grid. Ensemble averages of the fluid velocity measurements at specific stages of the grid’s oscillation indicate that mean flow is induced in OGT by the merging of grid-induced jets close to the tank sidewalls. The installation of an open-ended ‘inner box’ (with its top edge positioned just below the bottom of the grid’s oscillation) is shown to inhibit the merging of the jets, thereby resulting in a reduction in the magnitude of the mean flow within the interior of the inner box region. Measurements of the time-averaged root-mean-square turbulent velocity components and the time-averaged turbulent kinetic energy flux indicate that the installation of the inner box results in turbulence that is in good agreement with the well-established models of OGT across the central 50% of the inner box’s width, but that distinct anisotropic regions exist adjacent to the vertical sidewalls. We anticipate that this simple amendment to reduce the mean flow present in OGT can be readily used in future work that utilises OGT to isolate the effects of turbulent fluctuations from those of the mean flow

Serum 25-hydroxy vitamin D: a predictor of macrovascular and microvascular complications in patients with type 2 diabetes

Objective People with diabetes frequently develop vascular disease. We investigated the relationship between blood 25-hydroxy vitamin D (25OH-D) concentration and vascular disease risk in type 2 diabetes. Research design and methods The relationships between blood 25OH-D concentration at baseline and the incidence of macrovascular (including myocardial infarction, stroke) and microvascular (retinopathy, nephropathy, neuropathy, and amputation) disease were analysed with Cox proportional-hazards models and logistic regression in an observational study of patients in the 5-year Fenofibrate Intervention and Event Lowering in Diabetes trial. Results 50% of the patients had low vitamin D concentrations, as indicated by median blood 25OH-D concentration of 49nmol/L. These patients with a blood 25OH-D concentration < 50nmol/L had a higher cumulative incidence of macrovascular and microvascular events than those with levels ≥ 50nmol/L. Multivariate analysis, stratified by treatment and adjusted for relevant confounders, identified blood 25OH-D concentration as an independent predictor of macrovascular events. A 50nmol/L difference in blood 25OH-D concentration was associated with a 23% (P=0.007) change in risk of macrovascular complications during the study and further adjustments for seasonality, hs-CRP and physical activity level had little impact. The unadjusted risk of microvascular complications was 18% (P=0.006) higher during the study, though the excess risk declined to 11-14% and lost significance with adjustment for HbA1C, seasonality or physical activity. Conclusions Low blood 25OH-D concentrations are associated with an increased risk of macrovascular and microvascular disease events in type 2 diabetes. However, a causal link remains to be demonstrated

Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence

Galactosyltransferase (EC 2.4.1.22), purified to homogeneity from human milk by affinity chromatography, had an apparent molecular weight of 53,000 as determined by denaturing polyacrylamide gel electrophoresis. Subtration of the estimated contribution of the oligosaccharide portion of the molecule leaves a Mr of 47,000. An N-terminal amino acid sequence analysis of the isolated protein revealed a sequence similar to that found near the 5' end of a cDNA clone isolated by Shaper et al (11), which encodes a 35,500 molecular weight protein. Either the molecular weight of galactosyltransferase, has been overestimated, or a discrepancy exists between the actual molecular weight of galactosyltransferase and that predicted by the bovine cDNA clone isolated by Shaper et al (11).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26099/1/0000175.pd

High prevalence of early repolarization in the paediatric relatives of sudden arrhythmic death syndrome victims and in normal controls

AIMS: Elevation of the ECG J-point in the inferior and lateral leads (early repolarization) has been described in survivors of ventricular fibrillation (VF) arrest and occurs in adult first-degree relatives of sudden cardiac death (SCD) probands at a frequency significantly greater than in controls, raising the possibility that this could represent an independent risk factor in the aetiology of SCD. However, data on early repolarization in the paediatric population are lacking. This study aimed to assess the prevalence of early repolarization in paediatric first-degree relatives of sudden arrhythmic death syndrome (SADS) victims. METHODS AND RESULTS: Paediatric relatives (aged 1 mV from baseline. The ECGs of 77 consecutive paediatric first-degree relatives of SADS victims from 46 families were reviewed by two assessors. J-point elevation was present in 24 patients (31%) of this patient group compared with the reported prevalence of 5–13% in the published general paediatric population (P = 0.02) and that of 19% in the internal control group (P = 0.07). Subgroup analysis according to J-point elevation and ST segment morphologies showed a significantly higher prevalence of inferior early repolarization 0.1–0.2 mV in the study group compared with controls (75 vs. 38%; P = 0.02). CONCLUSION: Inferolateral J-point elevation occurs in a substantial proportion of paediatric first-degree relatives of SADS probands with a similar prevalence to that described in adults. This suggests that early repolarization could be an important inherited trait when evaluating relatives of SADS victims. However, prospective follow-up of this group of children is important to establish the implication of this finding in future risk stratification, given the apparently high prevalence in normal individuals

Isolation of a cDNA coding for human galactosyltransferase

Human milk galactosyltransferase (EC 2.4.1.22) was purified to homogeneity using affinity chromatography. Edman degradation was used to determine the amino acid sequences of eight peptide fragments isolated from the purified enzyme. A 60-mer "optimal" oligonucleotide probe that corresponded to the amino acid sequence of one of the galactosyltransferase peptide fragments was constructed and used to screen a [lambda]gt10 cDNA library. Two hybridization-positive recombinant phages, each with a 1.7 Kbp insert, were detected among 3 x 106 recombinant [lambda]gt10 phages. Sequencing of one of the cDNA inserts revealed a 783 bp galactosyltransferase coding sequence. The remainder of the sequence corresponded to the 3'-region of the mRNA downstream from the termination codon.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26068/1/0000142.pd

The International Law of Secession and the Protection of the Human Rights of Oppressed Sub-State Groups: Yesterday, Today and Tomorrow

This paper focuses on significant patterns/features in the historical development of the international law of secession and its contribution over time (or the lack thereof) to the struggle to afford greater protection to oppressed sub-state groups the world over. It was Crawford Young who once observed that “the state as an analytical quarry is an elusive and complex prey.” With the necessary modifications, this observation applies with almost equal force to the international law of secession. Complexity and confusion loom too large in this area of international law. For example, there is, at best, little clarity in the literature of the discipline of international law and in related fields of study regarding the existence or otherwise of an international legal entitlement to secession in favor of even the most highly oppressed and subjugated sub-state groups

Gene Expression Changes Associated with the Airway Wall Response to Injury

Understanding the way in which the airway heals in response to injury is fundamental to dissecting the mechanisms underlying airway disease pathology. As only limited data is available in relation to the in vivo characterisation of the molecular features of repair in the airway we sought to characterise the dynamic changes in gene expression that are associated with the early response to physical injury in the airway wall.We profiled gene expression changes in the airway wall using a large animal model of physical injury comprising bronchial brush biopsy in anaesthetised sheep. The experimental design featured sequential studies in the same animals over the course of a week and yielded data relating to the response at 6 hours, and 1, 3 and 7 days after injury. Notable features of the transcriptional response included the early and sustained preponderance of down-regulated genes associated with angiogenesis and immune cell activation, selection and differentiation. Later features of the response included the up-regulation of cell cycle genes at d1 and d3, and the latter pronounced up-regulation of extracellular matrix-related genes at d3 and d7.It is possible to follow the airway wall response to physical injury in the same animal over the course of time. Transcriptional changes featured coordinate expression of functionally related genes in a reproducible manner both within and between animals. This characterisation will provide a foundation against which to assess the perturbations that accompany airway disease pathologies of comparative relevance