107 research outputs found
Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator
We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 ”m thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW
Fruit-eating fishes of Banara arguta (Salicaceae) in the Miranda River floodplain, Pantanal wetland
Resource partitioning and ecomorphological variation in two syntopic species of Lebiasinidae (Characiformes) in an Amazonian stream
Compartilhamento de recursos por duas espécies de peixes nectobentÎnicas de riachos na bacia do rio Cachoeira (BA)
Preparing for Critical Infrastructure Breakdowns: The Limits of Crisis Management and the Need for Resilience
Composição da ictiofauna em função da fisiografia de um riacho costeiro de Floresta Atlùntica - Brasil
Ichthyofauna of Rio Jurubatuba, Santos, SĂŁo Paulo: a high diversity refuge in impacted lands
Trophic ecology of Hemigrammus marginatus Ellis, 1911 (Characiformes, Characidae) in a conserved tropical stream
Uso de recursos alimentares por peixes imaturos e adultos de espĂ©cies piscĂvoras em uma planĂcie de inundação neotropical
Schizophrenia-associated somatic copy-number variants from 12,834 cases reveal recurrent NRXN1 and ABCB11 disruptions
While germline copy-number variants (CNVs) contribute to schizophrenia (SCZ) risk, the contribution of somatic CNVs (sCNVs)âpresent in some but not all cellsâremains unknown. We identified sCNVs using blood-derived genotype arrays from 12,834 SCZ cases and 11,648 controls, filtering sCNVs at loci recurrently mutated in clonal blood disorders. Likely early-developmental sCNVs were more common in cases (0.91%) than controls (0.51%, p = 2.68eâ4), with recurrent somatic deletions of exons 1â5 of the NRXN1 gene in five SCZ cases. Hi-C maps revealed ectopic, allele-specific loops forming between a potential cryptic promoter and non-coding cis-regulatory elements upon 5âČ deletions in NRXN1. We also observed recurrent intragenic deletions of ABCB11, encoding a transporter implicated in anti-psychotic response, in five treatment-resistant SCZ cases and showed that ABCB11 is specifically enriched in neurons forming mesocortical and mesolimbic dopaminergic projections. Our results indicate potential roles of sCNVs in SCZ risk
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