Functional anatomy of the horse foot (1999)

Reviewed and reprinted 10/99/5M

Genome wide association study of passive immunity and disease traits in beef-suckler and dairy calves on Irish farms

peer reviewedCalves with lower concentrations of immunoglobulin G (IgG) in their blood, have a greater risk of developing diseases. There is a lack of knowledge on genetic markers known to be associated with immunological variability or disease resistance. Therefore, the objective of this study was to identify SNP markers associated with passive immunity measures (serum IgG, serum protein, albumin, globulin and total protein concentrations, total solids Brix percentage, zinc sulphate turbidity units) and disease (pneumonia, diarrhoea, crude illness) traits in Irish commercial beef-suckler and dairy calves through genome wide association studies (GWAS). Genotyping was performed on DNA samples from beef-suckler (n = 698) and dairy (n = 1178) calves, using the IDBv3 chip. Heritability of passive immunity associated traits (range 0.02–0.22) and the disease traits (range 0.03–0.20) were low-to-moderate. Twenty-five and fifteen SNPs approached genome wide significance (P < 5 × 10−5) for the passive immunity and the disease traits, respectively. One SNP “ARS-BFGL-BAC-27914” reached Bonferroni genome wide significance (P < 1.15 × 10−6) for an association with serum IgG concentration in beef calves. Further work will evaluate these SNPs in larger cattle populations and assess their contribution to genomic selection breeding strategies, aimed towards producing more disease resistant livestock.Department of Agriculture, Food and the Marine, Irelan

The effect of impurity gasses on variable polarity plasma arc welded 2219 aluminum

Variable polarity plasma arc (VPPA) welding has been used with considerable success by NASA for the welds on the Space Shuttle External Tank as well as by others concerned with high quality welded structures. The effects of gaseous contaminants on the appearance of VPPA welds on 2219 aluminum are examined so that a welder can recognize that such contamination is present and take corrective measures. There are many possible sources of such contamination including, contaminated gas bottles, leaks in the gas plumbing, inadequate shield gas flow, condensed moisture in the gas lines or torch body, or excessive contaminants on the workpiece. The gasses chosen for study in the program were nitrogen, oxygen, methane, and hydrogen. Welds were made in a carefully controlled environment and comparisons were made between welds with various levels of these contaminants and welds made with research purity (99.9999 percent) gasses. Photographs of the weld front and backside as well as polished and etched cross sections are presented

Assessment of DNA extracted from FTA® cards for use on the Illumina iSelect BeadChip

<p>Abstract</p> <p>Background</p> <p>As FTA^®cards provide an ideal medium for the field collection of DNA we sought to assess the quality of genomic DNA extracted from this source for use on the Illumina BovineSNP50 iSelect BeadChip which requires unbound, relatively intact (fragment sizes ≥ 2 kb), and high-quality DNA. Bovine blood and nasal swab samples collected on FTA cards were extracted using the commercially available GenSolve kit with a minor modification. The call rate and concordance of genotypes from each sample were compared to those obtained from whole blood samples extracted by standard PCI extraction.</p> <p>Findings</p> <p>An ANOVA analysis indicated no significant difference (P > 0.72) in BovineSNP50 genotype call rate between DNA extracted from FTA cards by the GenSolve kit or extracted from whole blood by PCI. Two sample t-tests demonstrated that the DNA extracted from the FTA cards produced genotype call and concordance rates that were not different to those produced by assaying DNA samples extracted by PCI from whole blood.</p> <p>Conclusion</p> <p>We conclude that DNA extracted from FTA cards by the GenSolve kit is of sufficiently high quality to produce results comparable to those obtained from DNA extracted from whole blood when assayed by the Illumina iSelect technology. Additionally, we validate the use of nasal swabs as an alternative to venous blood or buccal samples from animal subjects for reliably producing high quality genotypes on this platform.</p

The Open Cluster NGC 7789: I. Radial Velocities for Giant Stars

A total of 597 radial-velocity observations for 112 stars in the ~1.6 Gyr old open cluster NGC 7789 have been obtained since 1979 with the radial velocity spectrometer at the Dominion Astrophysical Observatory. The mean cluster radial velocity is -54.9 +/- 0.12 km/s and the dispersion is 0.86 km/s, from 50 constant-velocity stars selected as members from this radial-velocity study and the proper motion study of McNamara and Solomon (1981). Twenty-five stars (32%) among 78 members are possible radial-velocity variable stars, but no orbits are determined because of the sparse sampling. Seventeen stars are radial-velocity non-members, while membership estimates of six stars are uncertain. There is a hint that the observed velocity dispersion falls off at large radius. This may due to the inclusion of long-period binaries preferentially in the central area of the cluster. The known radial-velocity variables also seem to be more concentrated toward the center than members with constant velocity. Although this is significant at only the 85% level, when combined with similar result of Raboud and Mermilliod (1994) for three other clusters, the data strongly support the conclusion that mass segregation is being detected.Comment: 16 pages (including 3 figures) and 3 table