Immunohistochemistry in Irradiated Skin Tissue

Currently there is no effective treatment for radiation dermatitis that results from clinical or accidental radiation exposures. Radiation exposure can cause severe burns and sloughing of the skin and damage muscle and bone layers underneath the skin. Radiation exposure in cells results in several types of cell death, such as necrosis, apoptosis, or autophagy, or accelerated senescence. Preliminary experiments demonstrated that accelerated senescence is a primary response to radiation in normal skin cells in culture and skin tissue in vivo in mice. We wanted to use immunohistochemistry to identify the skin cells that undergo senescence in tissues obtained from 4 mice over a time course from 1-30 days following exposure to 17.9 Gy (0.6 Gy/min) irradiation. The different stains that are going to be used are hematoxylin and eosin stain which shows the morphology of the whole tissue, K15 which marks adult skin epidermal stem cells, p21/waf1 which is a marker for senescence, DCT which marks melanocyte stem cells, and c-kit which marks melanocytes and basal epithelial cells. The results from these experiments will help us to determine which cells to protect in order to treat severe radiation exposure

Mild Traumatic Brain Injuries

Mild traumatic brain injury (mTBI) or concussion is a serious health problem. Brain injuries can occur due to a variety of impacts and can occur at any age. While concussions are currently in the news, information regarding recognition, response, and management are frequently out of date Literature was reviewed in relation to current research in the pathophysiology of mTBI and focused in the area of cerebral blood flow, metabolic demands, and neuronal damage. Additionally, current recommendations relating to identification and response were reviewed in relation to athletics and in the military setting. Nursing knowledge was also addressed by a review of the literature, assessment of two nursing programs, and evaluation of several text books used in nursing education In response to current infom1ation available to school nursing in this community, a presentation was developed. This presentation reviewed current research in the pathophysiology of mTBI and the recommended guidelines in response and management There are many areas in which additional information for mTBI is needed. This is true in nursing text books, nursing programs, and for nurses currently in practice. One of the ways change will occur is the increasing number of laws that are being enacted relating to mTBI and student athletes. This will create a more immediate need for nurses to have comprehensive knowledge of mTBI, in both educational and community setting

An evaluation of the first two years of a secondary school reading program: 1955-56, 1956-57

Thesis (Ed.M.)--Boston Universit

Evaluating work place wellness in greater Louisville\u27s technology organizations : a case study.

Employers in the Southern Indiana and Greater Louisville region are looking for strategies to help them become a healthier workplace. Many employers see the expense of paying for an unhealthy workforce and they are looking to limit these expenses. The purpose of the study is to determine the state of workplace wellness activities in organizations in Southern Indiana and Greater Louisville. Due to the poor health statuses of Indiana and Kentucky, 39th and 45th, respectively, this study is significant for a number of stakeholders in our area. The purpose of this case study was to understand the state of health and wellness of organizations in Southern Indiana and Greater Louisville, according to the Centers for Disease Control Health Scorecard (CDC, 2014). Participants were first given the Centers for Disease Control Health Scorecard (CDC HSC) to develop a consistent quantitative baseline. The CDC HSC is a 125 question, 264-point survey that covers a diverse set of work place wellness initiatives. During the survey, participants were encouraged to elaborate on any aspect of the CDC HSC, such as organizational structure, tobacco control, physical activity, or any of the other categories included. Participants were then asked a series of open-ended interview questions to explore the ways they are, or are not, addressing the health and wellness in their workplace. The population from the study includes organizations from Southern Indiana and Greater Louisville. The results of the interviews were transcribed and compared to themes in the literature for patterns, themes, and outliers. Twenty-five organizations were included in the study, including a sole proprietorship that shares the thoughts of a mid-20s entrepreneur in the technology industry. The individuals in the study phase have a variety of roles in their organizations, so it is assumed they represent the majority of the workforce in their knowledge of the wellness programs. The pilot study focused on managers and human resources personnel and the researcher felt they were biased in their knowledge of the programs. The organizations vary in size, by industry, for-profit versus non-profit, and in the positions of the respondents. The worksite wellness information is organized in the same order as the categories of the Centers for Disease Control Health Scorecard (CDC HSC). The interview material is presented by sharing the data from high, middle, and low-scoring organizations, respectively. This study found that the importance of leveraging the knowledge of experts can increase the HSC score. Additionally, a variety of wellness programs lead to more employee engagement to help employees find a method of engaging that suits them. Organizations that remove obstacles and open doors can allow healthy actions to naturally take place, through Choice Architecture without coercing or incentivizing employees to participate. Safety was a major theme in the study, even when other aspects of wellness were not present. Smoking and chronic disease management were major challenges for organizations, even when they had robust wellness programs and scored highly on the CDC HSC

Discussion of unilateral spatial inattention and a proposed new screening method for its detection

Unilateral spatial inattention, also known as neglect, is a condition associated with brain injury that results in the patient being unaware of one hemifield, usually contralateral to the side of the lesion. It is commonly the result of traumatic or acquired brain injury (stroke) and it can be quite variable in its presentation and severity. Unilateral spatial inattention typically requires an extensive battery of tests to confirm its presence or absence, and thus is often not identified by individuals working with these patients. This lack of recognition often leaves unilateral spatial inattention patients underserved with their visual needs. In this paper we review some of the common definitions, causes, rates of occurrence and different manifestations of unilateral spatial inattention. We further review some of the classifications of unilateral spatial inattention as well as the more common tests used to detect and diagnose it. An area of much debate, treatment of unilateral spatial inattention, is also examined along with the clinical prognosis for unilateral spatial inattention patients. In this discussion we propose a new testing method to aid in the detection of unilateral spatial inattention. Brain injury patients commonly present with visual perceptual deficits for which The Test of Visual Perceptual Slulls non-motor is commonly administered. This test is frequently used by rehabilitation specialists and optometrists to help identify these deficits. We postulate that patients with unilateral spatial inattention will show more mistakes on those test plates where the correct answer choice falls into the neglected hemi-field. Therefore, it may be possible to uncover and diagnose unilateral spatial inattention solely by analyzing a patient\u27s pattern of errors on the Test of Visual Perceptual Skills. If it were easier to detect unilateral spatial inattention, it would likely lead to better rehabilitative care of unilateral spatial inattention patients

SiteWit Corporation: SQL or NoSQL that is the Question

This teaching case focuses on a start-up company in the Web analytics and online advertising space, which faces a database scaling challenge. The case covers the rapidly emerging NoSQL database products that can be used to implement very large distributed databases. These are exciting times in the database marketplace, with a flock of new companies offering scalable database systems for the cloud. These products challenge the existing relational database vendors that have come to dominate the market. The case outlines four potential solutions and asks students to make a choice or suggest a different alternative

Insecticide resistance in Drosophila melanogaster and Ctenocephalides felis

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