22 research outputs found
Small oligonucleotides detection in three-dimensional polymer network of dna-peg hydrogels
The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecule detection is needed. Here we investigate conditions to obtain a tailored hydrogel network that combines both molecular filtering and molecular capture capabilities for biosensing applications. Along this line, short oligonucleotide detection in a displacement assay is set within PEGDA hydrogels synthetized by UV radical photopolymerization. To provide insights on the molecular filter capability, diffusion studies of several probes (sulforho-damine G and dextrans) with different hydrodynamic radii were carried out using NMR technique. Moreover, fluorometric analyses of hybridization of DNA oligonucleotides inside PEGDA hydrogels shed light on the mechanisms of recognition in 3D, highlighting that mesh size and crowding effect greatly impact the hybridization mechanism on a polymer network. Finally, we found the best probe density and diffusion transport conditions to allow the specific oligonucleotide capture and detection inside PEGDA hydrogels for oligonucleotide detection and the filtering out of higher molecular weight molecules
Analisi dei possibili approcci alla valutazione delle frequenze di rilascio da auna tubazione a seguito di Sisma
Convegno VGR 200
The homogeneisation of three-phase systems with high solid content in a stirred vessel using radial flow impellers
Effect of scale on crystallization kinetics of potassium sulphate in batch and continuous crystallizers
RouTrHaz: routing di minimo rischio nel trasporto stradale di sostanze pericolose
Convegno VGR 2