The impact of a 48-hour fast on SIRT1 and GCN5 in human skeletal muscle

The present study examined the impact of a 48 hour fast on the expression and activation status of SIRT1 and GCN5, and the relationship between SIRT1/GCN5 and the gene expression of PGC-1α, and the PGC-1α target PDK4, in the skeletal muscle of ten lean healthy men (age, 22.0 ± 1.5 years; VO2peak, 47.2 ± 6.7 mL/min/kg). Muscle biopsies and blood samples were collected 1 hour postprandial (Fed) and following 48 hours of fasting (Fasted). Plasma insulin (Fed, 80.8 ± 47.9 pmol/L; Fasted, not detected) and glucose (Fed, 4.36 ± 0.86; Fasted, 3.74 ± 0.25 mmol/L, p = 0.08) decreased, confirming participant adherence to fasting. Gene expression of PGC-1α decreased (pThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author