Evidence for an interplay between cell cycle progression and the initiation of differentiation between life cycle forms of African trypanosomes

Successful transmission of the African trypanosome between the mammalian host blood-stream and the tsetse fly vector involves dramatic alterations in the parasite's morphology and biochemistry. This differentiation through to the tsetse midgut procyclic form is accompanied by re-entry into a proliferative cell cycle. Using a synchronous differentiation model and a variety of markers diagnostic for progress through both differentiation and the cell cycle, we have investigated the interplay between these two processes. Our results implicate a relationship between the trypanosome cell cycle position and the perception of the differentiation signal and demonstrate that irreversible commitment to the differentiation occurs rapidly after induction. Furthermore, we show that re-entry into the cell cycle in the differentiating population is synchronous, and that once initiated, progress through the differentiation pathway can be uncoupled from progress through the cell cycle

Housing the Aging Baby Boomers: Implications for Local Policy

Most elderly want to age in place. Yet, most elderly live in suburban and rural communities ill-suited to meet the changing aging-related demands. This paper discusses various issues communities need to address when balancing the demands of aging baby boomers against those of younger households. Accommodating changes in life stage needs requires revising building and zoning codes to permit mixed use and mixed density development incorporating greater varieties of housing units and easier accessibility. Developing support arrangements for naturally occurring retirement communities will become important for state and local governments. A significant number of aging adults will move to locales with natural and augmented civic amenities. Such migration is double-edged; features that attract "gray gold" also attract needy elderly. Finally, affordable housing will be an issue for a growing number of elderly, calling for targeted tax and financial assistance policies for lower income elderly homeowners. Working Paper 08-0

Research in planetary Astronomy

The focus was on the study, via near infrared observations, of the outer planets and their satellites. In the last year, these observations have emphasized imaging observations using the Cassegrain infrared camera at the f/70 focus of the 200 inch Hale telescope

New Measurements of the Radio Photosphere of Mira based on Data from the JVLA and ALMA

We present new measurements of the millimeter wavelength continuum emission from the long period variable Mira ($o$ Ceti) at frequencies of 46 GHz, 96 GHz, and 229 GHz ($\lambda$~7 mm, 3 mm, and 1 mm) based on observations obtained with the Jansky Very Large Array (JVLA) and the Atacama Large Millimeter/submillimeter Array (ALMA). The measured millimeter flux densities are consistent with a radio photosphere model derived from previous observations, where flux density, $S_{\nu}\propto\nu^{1.86}$. The stellar disk is resolved, and the measurements indicate a decrease in the size of the radio photosphere at higher frequencies, as expected if the opacity decreases at shorter wavelengths. The shape of the radio photosphere is found to be slightly elongated, with a flattening of ~10-20%. The data also reveal evidence for brightness non-uniformities on the surface of Mira at radio wavelengths. Mira's hot companion, Mira B was detected at all three observed wavelengths, and we measure a radius for its radio-emitting surface of $\approx2.0\times10^{13}$ cm. The data presented here highlight the power of the JVLA and ALMA for the study of the atmospheres of evolved stars.Comment: Accepted to ApJ; 27 pages, 7 figure

Near Infrared Observations of a Redshift 5.34 Galaxy: Further Evidence for Dust Absorption in the Early Universe

Imaging at 1.25 and 2.20 microns has been obtained of the field containing the galaxy (RD1) found at redshift 5.34 by Dey et al.(1998). This galaxy has been detected at 1.25 microns, while the lower redshift (z=4.02) galaxy also found in the same field by Dey et al. was detected at both 1.25 and 2.20 microns. Comparison to stellar population synthesis models indicates that if RD1 is a young ( 0.5 mag) is indicated. Combined with observations of other high redshift systems, these data show that dust is likely to be an important component of young galaxies even at redshifts of z > 5. The extinction-corrected monochromatic luminosity of RD1 at 1500 angstroms is then a factor of about three larger than L(1500)* as determined by Dickinson (1998) for z ~ 3 starburst galaxies. The implied star formation rate in RD1, corrected for extinction, is ~ 50-100 solar masses per year.Comment: plain LaTex with 1 postscript figure. ApJ Letters, accepte

Infrared Astronomy

Several observational programs in infrared astronomy are described and significant findings are briefly discussed. The near infrared work concentrates largely on the use of the 5 m Hale telescope in spectroscopic and photometric studies of extragalactic sources. Observations of the P alpha line profile in a low redshift quasar, X-ray bursters, reflection nebula, and cataclysmic variables are included. Millimeter continuum observations of dust emission from quasars and galactic molecular clouds are also discussed. Finally, improvements to instrumentation are reported

The bloodstream differentiation - division of Trypanosoma brucei studied using mitochondrial markers

In the bloodstream of its mammalian host, the African trypanosome Trypanosoma brucei undergoes a life cycle stage differentiation from a long, slender form to a short, stumpy form. This involves three known major events: exit from a proliferative cell cycle, morphological change and mitochondrial biogenesis. Previously, models have been proposed accounting for these events (Matthews & Gull 1994a). Refinement of, and discrimination between, these models has been hindered by a lack of stage-regulated antigens useful as markers at the single-cell level. We have now evaluated a variety of cytological markers and applied them to investigate the coordination of phenotypic differentiation and cell cycle arrest. Our studies have focused on the differential expression of the mitochondrial enzyme dihydrolipoamide dehydrogenase relative to the differentiation-division of bloodstream trypanosomes. The results implicate a temporal order of events: commitment, division, phenotypic differentiation