Early to Mid-Miocene syn-extensional massive silicic volcanism in the Pannonian Basin (East-Central Europe): Eruption chronology, correlation potential and geodynamic implications

eruptions of compositionally diverse magmas during the Neogene to Quaternary. The long-lasting magmatic activity began with some of the most voluminous silicic eruptions in Europe for the last 20Myr. This paper describes the eruption chronology of this volcanic activity using new, high-quality zircon U-Pb dates, and provides the first estimates on the volume and areal distribution of the volcanic products, characterizes the magma composition and discusses the silicic magmatism in a region, where the continental lithosphere underwent significant extension. A thorough zircon geochronological study was conducted on samples collected from ignimbrites and pyroclastic fall deposits exposed in the Bükkalja Volcanic Field. In-situ LA-ICP-MS analysis on zircon grains provided a fast, cheap and accurate method for such detailed geochronological work, where the volcanic products occur in scattered outcrops that often have poor stratigraphic constraints. The interpreted eruption ages were determined from the youngest zircon age population within the samples and this methodology was validated by new single zircon CA-ID-TIMS dates and sanidine Ar-Ar ages. The volcanism covers about 4Myrs, from 18.2Ma to 14.4Ma and involved at least eight eruptive phases. Within this, four large eruption events were recognized at 14.358±0.015Ma (Harsány ignimbrite), 14.880±0.014Ma (Demjén ignimbrite), 16.816±0.059Ma (Bogács unit) and 17.055±0.024Ma (Mangó ignimbrite), which are found in areas across the Pannonian Basin and elsewhere in central Europe. Considering all the potential sources of silicic ash found in the Paratethys sub-basins around the Pannonian Basin and along the northern Alps and in central Italy, we suggest that they were probably derived almost exclusively from the Pannonian Basin as shown by zircon U-Pb dates presented in this paper and published comparable age data from several localities. The new eruption ages considerably refine the Early to Mid-Miocene chronostratigraphy of the Pannonian basin, where the extensive volcanoclastic horizons are used as important marker layers. The cumulative volume of the volcanic material formed during this 4Myr long silicic volcanism is estimated to be >4000km⁠3, consistent with a significant ignimbrite flare-up event. Zircon crystallization ages indicate magma intrusions and formations of magma reservoirs in the continental crust for prolonged period, likely >1Myr prior to the onset of the silicic volcanism accompanied with sporadic andesitic to dacitic volcanic activities. Mafic magmas were formed by melting of the thinned lithospheric mantle metasomatized previously by subduction-related fluids and emplaced at the crust-mantle boundary. They evolved further by assimilation and fractional crystallization to generate silicic magmas, which ascended into the pre-warmed upper crust and formed extended magma storage regions. Zircon Hf isotope and bulk rock Sr-Nd isotopic data indicate a sharp decrease of crustal and/or increase of asthenospheric mantle input after 16.2Ma, suggesting that by this time the crust, and the lithospheric mantle was considerably thinned. This magmatism appears to have had a structural relationship to tectonic movements characterized by strike-slip and normal faults within the Mid-Hungarian Shear Zone as well as vertical axis block rotations, when the two microplates were juxtaposed. Our new zircon ages helped to refine the age of two major block-rotation phases associated with faulting. This volcanism shows many similarities with other rift-related silicic volcanic activities such as the Taupo Volcanic Zone (New Zealand) and the Basin and Range Province (USA)

Archive of Darkness:William Kentridge's Black Box/Chambre Noire

Situating itself in histories of cinema and installation art, William Kentridge's Black Box/Chambre Noire (2005) raises questions about screens, exhibition space, site-specificity and spectatorship. Through his timely intervention in a debate on Germany’s colonial past, Kentridge’s postcolonial art has contributed to the recognition and remembrance of a forgotten, colonial genocide. This article argues that, by transposing his signature technique of drawings for projection onto a new set of media, Kentridge explores how and what we can know through cinematic projection in the white cube. In particular, his metaphor of the illuminated shadow enables him to animate archival fragments as shadows and silhouettes. By creating a multi-directional archive, Black Box enables an affective engagement with the spectres of colonialism and provides a forum for the calibration of moral questions around reparation, reconciliation and forgiveness

Modulation of SOCS protein expression influences the interferon responsiveness of human melanoma cells

<p>Abstract</p> <p>Background</p> <p>Endogenously produced interferons can regulate the growth of melanoma cells and are administered exogenously as therapeutic agents to patients with advanced cancer. We investigated the role of negative regulators of interferon signaling known as suppressors of cytokine signaling (SOCS) in mediating interferon-resistance in human melanoma cells.</p> <p>Methods</p> <p>Basal and interferon-alpha (IFN-α) or interferon-gamma (IFN-γ)-induced expression of SOCS1 and SOCS3 proteins was evaluated by immunoblot analysis in a panel of n = 10 metastatic human melanoma cell lines, in human embryonic melanocytes (HEM), and radial or vertical growth phase melanoma cells. Over-expression of SOCS1 and SOCS3 proteins in melanoma cells was achieved using the PINCO retroviral vector, while siRNA were used to inhibit SOCS1 and SOCS3 expression. Tyr⁷⁰¹-phosphorylated STAT1 (P-STAT1) was measured by intracellular flow cytometry and IFN-stimulated gene expression was measured by Real Time PCR.</p> <p>Results</p> <p>SOCS1 and SOCS3 proteins were expressed at basal levels in melanocytes and in all melanoma cell lines examined. Expression of the SOCS1 and SOCS3 proteins was also enhanced following stimulation of a subset of cell lines with IFN-α or IFN-γ. Over-expression of SOCS proteins in melanoma cell lines led to significant inhibition of Tyr⁷⁰¹-phosphorylated STAT1 (P-STAT1) and gene expression following stimulation with IFN-α (IFIT2, OAS-1, ISG-15) or IFN-γ (IRF1). Conversely, siRNA inhibition of SOCS1 and SOCS3 expression in melanoma cells enhanced their responsiveness to interferon stimulation.</p> <p>Conclusions</p> <p>These data demonstrate that SOCS proteins are expressed in human melanoma cell lines and their modulation can influence the responsiveness of melanoma cells to IFN-α and IFN-γ.</p

The link between volcanism and plutonism in epizonal magma systems; high-precision U–Pb zircon geochronology from the Organ Mountains caldera and batholith, New Mexico

The Organ Mountains caldera and batholith expose the volcanic and epizonal plutonic record of an Eocene caldera complex. The caldera and batholith are well exposed, and extensive previous mapping and geochemical analyses have suggested a clear link between the volcanic and plutonic sections, making this an ideal location to study magmatic processes associated with caldera volcanism. Here we present high-precision thermal ionization mass spectrometry U–Pb zircon dates from throughout the caldera and batholith, and use these dates to test and improve existing petrogenetic models. The new dates indicate that Eocene volcanic and plutonic rocks in the Organ Mountains formed from ~44 to 34 Ma. The three largest caldera-related tuff units yielded weighted mean [superscript 206]Pb/[superscript 238]U dates of 36.441 ± 0.020 Ma (Cueva Tuff), 36.259 ± 0.016 Ma (Achenback Park tuff), and 36.215 ± 0.016 Ma (Squaw Mountain tuff). An alkali feldspar granite, which is chemically similar to the erupted tuffs, yielded a synchronous weighted mean [superscript 206]Pb/[superscript 238]U date of 36.259 ± 0.021 Ma. Weighted mean [superscript 206]Pb/[superscript 238]U dates from the larger volume syenitic phase of the underlying Organ Needle pluton range from 36.130 ± 0.031 to 36.071 ± 0.012 Ma, and the youngest sample is 144 ± 20 to 188 ± 20 ka younger than the Squaw Mountain and Achenback Park tuffs, respectively. Younger plutonism in the batholith continued through at least 34.051 ± 0.029 Ma. We propose that the Achenback Park tuff, Squaw Mountain tuff, alkali feldspar granite and Organ Needle pluton formed from a single, long-lived magma chamber/mush zone. Early silicic magmas generated by partial melting of the lower crust rose to form an epizonal magma chamber. Underplating of the resulting mush zone led to partial melting and generation of a high-silica alkali feldspar granite cap, which erupted to form the tuffs. The deeper parts of the chamber underwent continued recharge and crystallization for 144 ± 20 ka after the final eruption. Calculated magmatic fluxes for the Organ Needle pluton range from 0.0006 to 0.0030 km3/year, in agreement with estimates from other well-studied plutons. The petrogenetic evolution proposed here may be common to many small-volume silicic volcanic systems

Iterative sorting reveals CD133+ and CD133- melanoma cells as phenotypically distinct populations

Background: The heterogeneity and tumourigenicity of metastatic melanoma is attributed to a cancer stem cell model, with CD133 considered to be a cancer stem cell marker in melanoma as well as other tumours, but its role has remained controversial. Methods: We iteratively sorted CD133+ and CD133- cells from 3 metastatic melanoma cell lines, and observed tumourigenicity and phenotypic characteristics over 7 generations of serial xeno-transplantation in NOD/SCID mice. Results: We demonstrate that iterative sorting is required to make highly pure populations of CD133+ and CD133- cells from metastatic melanoma, and that these two populations have distinct characteristics not related to the cancer stem cell phenotype. In vitro, gene set enrichment analysis indicated CD133+ cells were related to a proliferative phenotype, whereas CD133- cells were of an invasive phenotype. However, in vivo, serial transplantation of CD133+ and CD133- tumours over 7 generations showed that both populations were equally able to initiate and propagate tumours. Despite this, both populations remained phenotypically distinct, with CD133- cells only able to express CD133 in vivo and not in vitro. Loss of CD133 from the surface of a CD133+ cell was observed in vitro and in vivo, however CD133- cells derived from CD133+ retained the CD133+ phenotype, even in the presence of signals from the tumour microenvironment. Conclusion: We show for the first time the necessity of iterative sorting to isolate pure marker-positive and marker-negative populations for comparative studies, and present evidence that despite CD133+ and CD133- cells being equally tumourigenic, they display distinct phenotypic differences, suggesting CD133 may define a distinct lineage in melanoma

Supplemental Material: A temporal dissection of late Quaternary volcanism and related hazards within the Rio Grande rift and along the Jemez lineament of New Mexico, USA

40Ar/39Ar data tables and related figures.  </div

Surface uplift above the Jemez mantle anomaly in the past 4 Ma based on 40Ar/39Ar dated paleoprofiles of the Rio San Jose, New Mexico, USA

We combine 15 new 40Ar/39Ar ages with existing age constraints of basalts to investigate the incision and denudation history of the ~150-km-long Rio San Jose (RSJ) of west-central New Mexico (USA) over the past 4 Ma. Temporal and spatial scales of differential incision may help evaluate the relative importance of neotectonic, geomorphic and climatic forcings. The RSJ is a southeast-flowing river that orthogonally crosses the northeast-trending Jemez volcanic lineament, which is underlain by a zone of low-velocity mantle. Preserved basalt flows along the length of the river at different elevations that directly overlie river gravels are used to construct paleoprofiles of the RSJ and give insight into the differential incision history, which can test the hypothesis that epeirogenic uplift associated with the Jemez lineament influenced differential incision of the RSJ. Observations include (1) a northeast-trending graben along the central reach of the RSJ (El Malpais valley graben) which is parallel to the Jemez lineament, (2) the present-day east tilt of the originally west-flowing 3.7 Ma Mesa Lucero flow along the eastern edge of the Jemez lineament, and (3) modern profile convexities that are colocated with ca. 3 Ma paleoprofile convexities and are centered above the Jemez lineament. The arched ca. 3 Ma paleoprofile defined by the pre–Mount Taylor strath has greater convexity than younger profiles, suggesting neotectonic bowing of ~135 m (~50 m/Ma) in this reach over the past ~3 Ma relative to areas off axis of the Jemez lineament, in spite of graben subsidence and aggradational fill in this reach exceeding 100 m. Differential incision of the 184 ka Suwanee flow at the edge of the Colorado Plateau may be attributable to base-level fall in downstream reaches of the RSJ and/or headwater uplift, and more erosive climate in the past several hundred thousand years. However, these observations, when considered together, cannot be explained entirely by geomorphic or climatic forcings. Rather, they are best interpreted as resulting from surface uplift centered over the northeast-trending Jemez lineament, and our model suggests that both the faulting and broad bending may relate to mantle driven epeirogeny that caused differential river incision. Several interacting neotectonic and magmatic mechanisms may have contributed to postulated uplift. Magmatically driven geodynamic uplift forcings may include construction of the Mount Taylor stratovolcano just north of the RSJ that changed surface elevation by several kilometers at the volcanic peak itself. However, semisteady denudation and similar incision rates in other rivers in the region indicate that a regional erosional landscape was the primary driver of differential river incision over the past 5–8 Ma. Our focus on the pre–Mount Tayler RSJ paleoprofile reinforces this conclusion. Other mantle-related uplift mechanisms that may have generated mantle buoyancy include thermal buoyancy or magmatic inflation due to dike and sill networks related to the building of the Mount Taylor stratovolcano and eruption of Zuni-Bandera volcanic fields. Both could have contributed to uplift, but their relative importance is unknown. Broad epeirogenic uplift is also possible due to small-scale upper mantle convection beneath a thin elastic plate and resulting dynamic topography