Evaluation of extensional and torsional stiffness of single actin filaments by molecular dynamics analysis.

It is essential to investigate the mechanical behaviour of cytoskeletal actin filaments in order to understand their critical role as mechanical components in various cellular functional activities. These actin filaments consisting of monomeric molecules function in the thermal fluctuations. Hence, it is important to understand their mechanical behaviour on the microscopic scale by comparing the stiffness based on thermal fluctuations with the one experimentally measured on the macroscopic scale. In this study, we perform a large-scale molecular dynamics (MD) simulation for a half-turn structure of an actin filament. We analyse its longitudinal and twisting Brownian motions in equilibrium and evaluated its apparent extensional and torsional stiffness on the nanosecond scale. Upon increasing the sampling-window durations for analysis, the apparent stiffness gradually decreases and exhibits a trend to converge to a value that is close to the experimental value. This suggests that by extrapolating the data obtained in the MD analysis, we can estimate the experimentally determined stiffness on the microsecond to millisecond scales. For shorter temporal scales, the apparent stiffness is larger than experimental values, indicating that fast, local motions of the molecular structure are dominant. To quantify the local structural changes within the filament on the nanosecond scale and investigate the molecular mechanisms, such as the binding of the actin-regulatory proteins to the filaments, it is preferable to analyse the mechanical behaviour on the nanometre and nanosecond scales using MD simulation

External iliac venous aneurysm in a pregnant woman: a case report

AbstractWe report an external iliac venous aneurysm in a young pregnant woman who was diagnosed incidentally by ultrasound scanning. The aneurysm was successfully treated by tangential aneurysmectomy and lateral venorrhaphy. Primary iliac venous aneurysm is a rare vascular abnormality. The clinical significance of the disease is unknown. However, embolism, rupture, and thrombosis might occur as they can occur with popliteal venous aneurysm. In fact, three of four reported patients with iliac venous aneurysms had a thromboembolic event. For those reasons, prophylactic treatment is indicated. This is the first patient with an iliac venous aneurysm to be diagnosed without complication

Secondary outflow driven by the protostar Ser-emb 15 in Serpens

We present the detection of a secondary outflow associated with a Class I source, Ser-emb 15, in the Serpens Molecular Cloud. We reveal two pairs of molecular outflows consisting of three lobes, namely primary and secondary outflows, using ALMA 12CO and SiO line observations at a resolution of 318 au. The secondary outflow is elongated approximately perpendicular to the axis of the primary outflow in the plane of the sky. We also identify two compact structures, Sources A and B, within an extended structure associated with Ser-emb 15 in the 1.3 mm continuum emission at a resolution of 40 au. The projected sizes of Sources A and B are 137 au and 60 au, respectively. Assuming a dust temperature of 20 K, we estimate the dust mass to be 0.0024 Msun for Source A and 0.00033 Msun for Source B. C18O line data imply the existence of rotational motion around the extended structure, however, cannot resolve rotational motion in Source A and/or B, due to insufficient angular and frequency resolutions. Therefore, we cannot conclude whether Ser-emb 15 is a single or binary system. Thus, either Source A or B could drive the secondary outflow. We discuss two scenarios to explain the driving mechanism of the primary and secondary outflows: the Ser-emb 15 system is (1) a binary system composed of Source A and B or (2) a single star system composed of only Source A. In either case, the system could be a suitable target for investigating the disk and/or binary formation processes in complicated environments. Detecting these outflows should contribute to understanding complex star-forming environments, which may be common in the star-formation processes.Comment: 21 pages, 10 figures, Accepted for publication in the Astrophysical Journa