重合性蛋白質の設計に基づく機能性蛋白質高次構造体の創製

学位の種別: 課程博士審査委員会委員 : (主査)東京大学教授 津本 浩平, 東京大学教授 伊藤 耕一, 東京大学准教授 富田 野乃, 東京大学客員教授 本田 真也, 東京理科大学教授 和田 猛University of Tokyo(東京大学

Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate

AbstractProtein microarray is considered to be one of the key analytical tools for high-throughput protein function analysis. Here, we report that the Arabidopsis HY5 functions as a novel DNA-binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate. Furthermore, we show three applications using the microarray: (1) detection of autophosphorylation activity of DBtagged human protein kinases and inhibition of their activity by staurosporine, (2) specific cleavage of DBtagged proteins by a virus protease and caspase 3, and (3) detection of a protein–protein interaction between the DBtagged UBE2N and UBE2v1. Thus, this method may facilitate rapid functional analysis of a wide range of proteins

〔研究ノート〕 心理支援職の職務満足の理由 ―自由記述の質的検討から―

　　In this study, we categorize the reasons for job satisfaction among psychological support professionals. We asked 81 clinical psychologists and clinical developmental psychologists about their job satisfaction using a 6-point scale ranging from “very satisfied” to “not satisfied at all,” and asked them to freely explain their reasons. Of the 81 participants, 67 （82.7％） were satisfied with their job and 14 （17.3％） were not satisfied. The reasons for job satisfaction are divided into three categories: “chance to exercise one’s abilities and growth,” “employment environment,” and “human relations.” “Chance to exercise one’s abilities and growth” has four subcategories: “rewarding/achievement,” “self-growth through work,” “sense of achievement at the desired job,” and “utilization of specialized skills.” The second category, “employment environment” has four subcategories: “work-life balance,” “flexible working style,” “salary/welfare,” and “employment status.” The third category, “human relations” has three subcategories: “existence of colleague psychological support professionals,” “good relationships” and “cooperation with other professionals.” The contents of each of these categories reveal much about the characteristics of clinical psychological support professionals