Autoantibodies to low-density-lipoprotein-receptor-related protein 2 (LRP2) in systemic autoimmune diseases

We previously reported that autoantibodies (autoAbs) to the main epitope on CD69 reacted to its homologous amino acid sequence in low-density-lipoprotein-receptor-related protein 2 (LPR2), a multiligand receptor for protein reabsorption. In this study, we have investigated the prevalence, autoepitope distribution, and clinical significance of the autoAbs to LRP2 in patients with systemic autoimmune diseases. Using six recombinant proteins (F2–F7) for LRP2 and one for CD69, we detected autoAbs to LRP2 in sera of patients with rheumatoid arthritis (RA), systemic lupus erythematosus, Behçet's disease, systemic sclerosis, and osteoarthritis and then mapped autoepitopes by Western blotting. The autoAbs to LRP2 were detected in 87% of the patients with rheumatoid arthritis, 40% of those with systemic lupus erythematosus, 35% of those with systemic sclerosis, 15% of those with osteoarthritis, and 3% of those with Behçet's disease. Multiple epitopes on LRP2 were recognized by most of the anti-LRP2(+ )serum samples. All of the tested anti-CD69 autoAb(+ )samples reacted to LRP2-F3 containing the homologous sequence to the main epitope of CD69; however, only 38% of the anti-LRP2-F3(+ )samples reacted to CD69. Clinically, the existence of the autoAbs to LRP2-F4, -F5, and -F6 correlated with the presence of proteinuria in RA. This study revealed that LRP2 is a major autoantigen in RA. The autoAbs to LRP2 are probably produced by the antigen-driven mechanism and the autoimmunity to LRP2 may spread to include CD69. The anti-LRP2 autoAbs may play pathological roles by inhibiting the reabsorbing function of LRP2

H2O maser motions and the distance of the star forming region G192.16-3.84

We present the results of astrometic observations of H2O masers associated with the star forming region G192.16-3.84 with the VLBI Exploration of Radio Astrometry (VERA). The H2O masers seem to be associated with two young stellar objects (YSOs) separated by \sim1200 AU as reported in previous observations. In the present observations, we successfully detected an annual parallax of 0.66 \pm 0.04 mas for the H2 O masers, which corresponds to a distance to G192.16-3.84 of D = 1.52 \pm 0.08 kpc from the Sun. The determined distance is shorter than the estimated kinematic distance. Using the annual parallax distance and the estimated parameters of the millimeter continuum emission, we estimate the mass of the disk plus circumstellar cloud in the southern young stellar object to be 10.0+4.3M\cdot. We also estimate the galactocentric distance and the peculiar motion -3.6 of G192.16-3.84, relative to a circular Galactic rotation: R\star = 9.99 \pm 0.08 kpc, Z\star = -0.10 \pm 0.01 kpc, and (U\star,V\star,W\star)=(-2.8\pm1.0,-10.5\pm0.3,4.9\pm2.7)[kms-1]respectively. The peculiar motion of G192.16-3.84 is within that typically found in recent VLBI astrometric results. The angular distribution and three-dimensional velocity field of H2O maser features associated with the northern YSO indicate the existence of a bipolar outflow with a major axis along the northeast-southwest direction.Comment: 9 pages, 2 figures and 4 tables. Accepted for publication on PAS

Resource constrained distributed constraint optimization with virtual variables

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