Infectious Bronchitis Virus: a major cause of respiratory disease outbreaks in chickens in Ghana

Infectious Bronchitis Virus (IBV) causes respiratory diseases with significant economic losses in the global poultry industry. The possible presence of this pathogen in the commercial poultry industry in Ghana has not yet been evaluated and thus there is no control strategy in place. In the present study, the RT-PCR technique was used to detect IBV in respiratory disease outbreaks in 6 chicken farms in the Ga East district of Accra. RNA was extracted from tracheal swabs of birds with respiratory signs and used in an RT-PCR to amplify the S1 glycoprotein gene of IBV. Forty seven (47) samples were obtained and analyzed during a 3 month period from broilers, layers and broiler breeders. Thirty (30) of the samples were positive for IBV by RT-PCR, giving a prevalence of 64%. This study confirms the presence of IBV in the study area and provides initial evidence of IBV involvement in respiratory disease outbreaks. Additional studies including virus isolation attempts and characterization will provide necessary data to outline and implement an effective control strategy in the country. Keywords: Infectious Bronchitis Virus, RT-PCR, chickens, Ghan

Activity of Mycoplasma synoviae in Commercial Chickens in Ghana: Serological Evidence

A total of 390 chicken sera were collected from respiratory disease free broilers, breeders and layers in the Ga-East district of Ghana and tested for Mycoplasma synoviae (MS) antibodies using commercial enzyme-linked immunosorbent assay (ELISA) kit from IDEXX Inc. USA.  The overall sero-prevalence of MS was obtained to be 46.2%. The sero-prevalence among breeders and broilers were 0% each. MS sero-prevalence among layers ranged between 60% -85% and differed significantly among different ages (p< 0.05).  The overall MS sero-prevalence among layers was 75%. It is concluded that Mycoplasma synoviae circulate in layer farms in the district. Layer birds at peak production age are at higher risk of infection. Keywords: Mycoplasma synoviae, chicken, Sero-prevalence, Ghan

Widespread exposure to infectious bronchitis virus and Mycoplasma gallisepticum in chickens in the Ga-East district of Accra, Ghana

Infectious bronchitis, a major challenge to the global poultry industry, is an acute and highly contagious disease of the respiratory and urogenital tract of chickens which causes significant economic losses to poultry producers. In addition, Mycoplasma gallisepticum (MG) is another respiratory pathogen that remains a concern to producers. This study investigated the sero-prevalence of IBV and MG in commercial chickens in Ga-East district of the Greater Accra Region, Ghana, using sera obtained from 440 broiler and layer chickens showing no signs of disease. IBV and MG specific antibodies were determined using commercial ELISA kits. Majority (85%) of the samples tested positive for at least one of the 2 pathogens investigated, with 30% testing positive for both. Overall sero-prevalence of IBV and MG were 85.5 and 29.5% respectively suggesting a higher IBV than MG field challenge in the study area. IBV prevalence was significantly higher in layers (100%) than broilers (42%). Age of bird had a significant influence on IBV prevalence among broilers. The MG prevalence in layers and broilers were 39.4 and 0% respectively. This data supports the need to institute control measures to mitigate IBV associated losses and improve poultry production in Ghana

Antimicrobial Resistance of Escherichia coli from Broilers, Pigs, and Cattle in the Greater Kumasi Metropolis, Ghana

Globally, resistance to antimicrobial drugs in food animals is on the rise. Escherichia coli of livestock, though commensal in nature, serves as reservoir for antimicrobial resistance genes with the potential of disseminating them. This study sought to examine the antimicrobial resistance profiles of Escherichia coli in broilers, pigs, and cattle in the Kumasi Metropolis and undertake molecular characterisation of the resistances. Faecal E. coli isolates (n = 48) were obtained from 10 broiler farms, (n = 43) from 15 pig farms, and (n = 42) from cattle from the Kumasi Abattoir using standard bacteriological techniques. The Kirby–Bauer disc diffusion method was employed in testing the sensitivities of 133 E. coli isolates to 15 antimicrobials. All 48 isolates from broilers presented no resistance to amoxicillin/clavulanic acid and ceftiofur. A 100% resistance to meropenem was observed in pig and cattle isolates. Multidrug resistance (MDR) across animal groups was 95.8% (n = 46), 95.3% (n = 41), and 64.3% (n = 27) for broilers, pigs, and cattle, respectively. Twenty-eight isolates presenting phenotypic resistance to aminopenicillins and cephalosporins were screened for the presence of extended-spectrum beta-lactamase (ESBL) genes by PCR. One isolate from poultry and another from cattle tested positive for the blaCTX-M ESBL gene. There were no positives for the blaTEM and blaSHV ESBL genes. Commensal E. coli of food animal origin represents an important reservoir of antimicrobial resistance that transfers resistance to pathogenic and nonpathogenic microbes affecting humans and animals. There is an urgent need to institute routine surveillance for the establishment of the mechanisms and molecular orientation of resistance in these organisms

Antimicrobial, Multi-Drug and Colistin Resistance in Enterobacteriaceae in Healthy Pigs in the Greater Accra Region of Ghana, 2022: A Cross-Sectional Study

There is little published information on antimicrobial resistance (AMR) in animals in Ghana. We determined the prevalence and factors associated with AMR, multi-drug resistance (MDR-resistance to ≥3 antimicrobial classes) and colistin resistance in Enterobacteriaceae in healthy pigs in Accra, Ghana. Rectal swabs obtained from the pigs on 20 farms from January to March 2022, were examined for Escherichia coli, Enterobacter spp. and Klebsiella pneumoniae. AMR was determined using standard microbiological techniques and the mcr-1 gene detected through molecular analysis. Enterobacteriaceae were isolated from 197 of 200 pigs: these comprised 195 E. coli isolates, 38 Enterobacter spp. and 3 K. pneumoniae, either singly or combined. Over 60% of E. coli were resistant to tetracycline, with 27% and 34% being resistant to amoxicillin/clavulanic acid and ampicillin, respectively; 23% of E. coli and 5% of Enterobacter spp. exhibited MDR phenotypes. Phenotypic colistin resistance was found in 8% of E. coli and Enterobacter spp., with the mcr-1 gene detected in half. Our study findings should be incorporated into on-going AMR, MDR and colistin resistance surveillance programs in Ghana. We further advocate for tailored-specific education for pig farmers on animal antimicrobial use and for strengthened regulatory policy on antimicrobial usage and monitoring in the animal production industry