Limiting the impact of destructive analytical techniques through sequential microspatial sampling of the enamel from single teeth

A fundamental research concern within contemporary bioarchaeology is the sensitive balance between the preservation of human remains and the use of destructive techniques to collect information. Here we describe one example of how multiple microspatial destructive/semi-destructive techniques may be carried out in sequence using only the enamel of a single tooth. With careful planning of both sample preparation strategies and sequencing of sampling methods, it is possible to produce multiple datasets, and yet to retain material for future analyses. In this case, enamel from the teeth of 27 individuals who lived during the early medieval period (AD 1170-1198) in Bergen, Norway, were subjected to histological, trace element (LA-ICP-MS), diagenetic (FTIR), and isotopic analyses (δ18O and δ13C, via micromill/multiprep/IRMS)

Infrared studies of the Be star X Per

Photometric and spectroscopic results are presented for the Be star X Per/HD 24534 from near-infrared monitoring in 2010-2011. The star is one of a sample of selected Be/X-ray binaries being monitored by us in the near-IR to study correlations between their X ray and near-IR behaviour. Comparison of the star's present near-IR magnitudes with earlier records shows the star to be currently in a prominently bright state with mean J, H, K magnitudes of 5.49, 5.33 and 5.06 respectively. The JHK spectra are dominated by emission lines of HeI and Paschen and Brackett lines of HI. Lines of OI 1.1287 and 1.3165 micron are also present and their relative strength indicates, since OI 1.1287 is stronger among the two lines, that Lyman beta fluorescence plays an important role in their excitation. Recombination analysis of the HI lines is done which shows that the Paschen and Brackett line strengths deviate considerably from case B predictions. These deviations are attributed to the lines being optically thick and this supposition is verified by calculating the line center optical depths predicted by recombination theory. Similar calculations indicate that the Pfund and Humphrey series lines should also be expected to be optically thick which is found to be consistent with observations reported in other studies. The spectral energy distribution of the star is constructed and shown to have an infrared excess. Based on the magnitude of the IR excess, which is modeled using a free-free contribution from the disc, the electron density in the disc is estimated and shown to be within the range of values expected in Be star discs.Comment: Accepted for publication in MNRAS, 7 pages, 6 figure

Genetic Variants in P-Selectin and C-Reactive Protein Influence Susceptibility to Cognitive Decline After Cardiac Surgery

ObjectivesWe hypothesized that candidate gene polymorphisms in biologic pathways regulating inflammation, cell matrix adhesion/interaction, coagulation-thrombosis, lipid metabolism, and vascular reactivity are associated with postoperative cognitive deficit (POCD).BackgroundCognitive decline is a common complication of coronary artery bypass graft (CABG) surgery and is associated with a reduced quality of life.MethodsIn a prospective cohort study of 513 patients (86% European American) undergoing CABG surgery with cardiopulmonary bypass, a panel of 37 single-nucleotide polymorphisms (SNPs) was genotyped by mass spectrometry. Association between these SNPs and cognitive deficit at 6 weeks after surgery was tested using multiple logistic regression accounting for age, level of education, baseline cognition, and population structure. Permutation analysis was used to account for multiple testing.ResultsWe found that minor alleles of the CRP1059G/C SNP (odds ratio [OR] 0.37, 95% confidence interval [CI] 0.16 to 0.78; p = 0.013) and the SELP1087G/A SNP (OR 0.51, 95% CI 0.30 to 0.85; p = 0.011) were associated with a reduction in cognitive deficit in European Americans (n = 443). The absolute risk reduction in the observed incidence of POCD was 20.6% for carriers of the CRP1059C allele and 15.2% for carriers of the SELP1087A allele. Perioperative serum C-reactive protein (CRP) and degree of platelet activation were also significantly lower in patients with a copy of the minor alleles, providing biologic support for the observed allelic association.ConclusionsThe results suggest a contribution of P-selectin and CRP genes in modulating susceptibility to cognitive decline after cardiac surgery, with potential implications for identifying populations at risk who might benefit from targeted perioperative antiinflammatory strategies

Attention Restoration Theory: A Systematic Review of the Attention Restoration Potential of Exposure to Natural Environments

All authors contributed to the design of this review, critically revised the article, and approved the final versions. HO contributed to all stages of the systematic review (searching, screening, data extraction, quality appraisal and synthesis) and drafted the article. MW and BW contributed to double data extraction and preparation of the article. AB devised the search strategy, ran the literature searches, carried out citation searching, and contributed to double screening. OU and VN provided statistical advice and designed and conducted the meta-analyses. RG conceived the idea for the review, contributed to double screening, double data extraction, quality appraisal, and preparation of the article, and is the guarantor

Quantifying the UK's carbon dioxide flux: An atmospheric inverse modelling approach using a regional measurement network

We present a method to derive atmosphericobservation-based estimates of carbon dioxide (CO 2 ) fluxes at the national scale, demonstrated using data from a network of surface tall-tower sites across the UK and Ireland over the period 2013-2014. The inversion is carried out using simulations from a Lagrangian chemical transport model and an innovative hierarchical Bayesian Markov chain Monte Carlo (MCMC) framework, which addresses some of the traditional problems faced by inverse modelling studies, such as subjectivity in the specification of model and prior uncertainties. Biospheric fluxes related to gross primary productivity and terrestrial ecosystem respiration are solved separately in the inversion and then combined a posteriori to determine net ecosystem exchange of CO 2 . Two different models, Data Assimilation Linked Ecosystem Carbon (DALEC) and Joint UK Land Environment Simulator (JULES), provide prior estimates for these fluxes. We carry out separate inversions to assess the impact of these different priors on the posterior flux estimates and evaluate the differences between the prior and posterior estimates in terms of missing model components. The Numerical Atmospheric dispersion Modelling Environment (NAME) is used to relate fluxes to the measurements taken across the regional network. Posterior CO2 estimates from the two inversions agree within estimated uncertainties, despite large differences in the prior fluxes from the different models. With our method, averaging results from 2013 and 2014, we find a total annual net biospheric flux for the UK of 8±79 TgCO 2 yr -1 (DALEC prior) and 64±85 TgCO 2 yr -1 (JULES prior), where negative values represent an uptake of CO 2 . These biospheric CO 2 estimates show that annual UK biospheric sources and sinks are roughly in balance. These annual mean estimates consistently indicate a greater net release of CO 2 than the prior estimates, which show much more pronounced uptake in summer months

Longitudinal effects on mental health of moving to greener and less green urban areas

This is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record.Despite growing evidence of public health benefits from urban green space there has been little longitudinal analysis. This study used panel data to explore three different hypotheses about how moving to greener or less green areas may affect mental health over time. The samples were participants in the British Household Panel Survey with mental health data (General Health Questionnaire scores) for five consecutive years, and who relocated to a different residential area between the second and third years (n = 1064; observations = 5320). Fixed-effects analyses controlled for time-invariant individual level heterogeneity and other area and individual level effects. Compared to premove mental health scores, individuals who moved to greener areas (n = 594) had significantly better mental health in all three postmove years (P = .015; P = .016; P = .008), supporting a "shifting baseline" hypothesis. Individuals who moved to less green areas (n = 470) showed significantly worse mental health in the year preceding the move (P = .031) but returned to baseline in the postmove years. Moving to greener urban areas was associated with sustained mental health improvements, suggesting that environmental policies to increase urban green space may have sustainable public health benefits.European Regional Development Fund 2007-2013European Social Fund Convergence Programme for Cornwall and the Isles of ScillyEconomic and Social Research Counci

In-situ characterization of the Hamamatsu R5912-HQE photomultiplier tubes used in the DEAP-3600 experiment

The Hamamatsu R5912-HQE photomultiplier-tube (PMT) is a novel high-quantum efficiency PMT. It is currently used in the DEAP-3600 dark matter detector and is of significant interest for future dark matter and neutrino experiments where high signal yields are needed. We report on the methods developed for in-situ characterization and monitoring of DEAP's 255 R5912-HQE PMTs. This includes a detailed discussion of typical measured single-photoelectron charge distributions, correlated noise (afterpulsing), dark noise, double, and late pulsing characteristics. The characterization is performed during the detector commissioning phase using laser light injected through a light diffusing sphere and during normal detector operation using LED light injected through optical fibres

Potential for pancreatic maturation of differentiating human embryonic stem cells is sensitive to the specific pathway of definitive endoderm commitment

This study provides a detailed experimental and mathematical analysis of the impact of the initial pathway of definitive endoderm (DE) induction on later stages of pancreatic maturation. Human embryonic stem cells (hESCs) were induced to insulin-producing cells following a directed-differentiation approach. DE was induced following four alternative pathway modulations. DE derivatives obtained from these alternate pathways were subjected to pancreatic progenitor (PP) induction and maturation and analyzed at each stage. Results indicate that late stage maturation is influenced by the initial pathway of DE commitment. Detailed quantitative analysis revealed WNT3A and FGF2 induced DE cells showed highest expression of insulin, are closely aligned in gene expression patterning and have a closer resemblance to pancreatic organogenesis. Conversely, BMP4 at DE induction gave most divergent differentiation dynamics with lowest insulin upregulation, but highest glucagon upregulation. Additionally, we have concluded that early analysis of PP markers is indicative of its potential for pancreatic maturation. © 2014 Jaramillo et al