Changes in the composition and fatty acid profile of Najdi ewes’ milk before and after weaning

This trial aimed to study the changes in the yield, composition, and fatty acid (FA) profile of ewes’ milk during suckling and milking periods. Nineteen multiparous Najdi ewes were kept under identical management and feeding conditions during a 12-week trial that was divided into two periods. Milk samples were collected on each sampling day from the total yield during the suckling (3rd, 6th, and 9th week) and milking (12th week) periods. The milk yield and the total solids, fat, protein, and lactose contents of the milk remained constant throughout the suckling weeks, but the protein content increased and milk yield and lactose content decreased after weaning. There were no differences in milk saturated FA (SFA), unsaturated FA (UFA), and monounsaturated FA (MUFA) contents during the suckling and milking periods, whereas the polyunsaturated FA content increased during the milking period. There were no differences in the individual milk SFA during the weeks of suckling, but the contents of caproic acid (C6:0), caprylic acid (C8:0), margaric acid (C17:0), and stearic acid (C18:0) decreased. However, myristic acid (C14:0), lauric acid (C12:0), and isomers of pentadecanoic (C15:0) acid increased during the milking period. The percentage of vaccenic acid (C18:1△11t), rumenic acid (C18:2△9c,11t; conjugated linoleic acid (CLA)), ω-3 FA group, and the atherogenicity index did not differ during the suckling period, but increased after weaning in the 12th week of lactation. This study demonstrated that weaning lambs in Najdi ewes is probably a factor that strongly affects milk traits by changing yield, composition, and FA profile.Keywords: CLA, dairy ewes, milk fat, milk yield, stage of lactatio

APOL1 genotype-associated morphologic changes among patients with focal segmental glomerulosclerosis

Background: The G1 and G2 alleles of apolipoprotein L1 (APOL1) are common in the Black population and associated with increased risk of focal segmental glomerulosclerosis (FSGS). The molecular mechanisms linking APOL1 risk variants with FSGS are not clearly understood, and APOL1’s natural absence in laboratory animals makes studying its pathobiology challenging. Methods: In a cohort of 90 Black patients with either FSGS or minimal change disease (MCD) enrolled in the Nephrotic Syndrome Study Network (58% pediatric onset), we used kidney biopsy traits as an intermediate outcome to help illuminate tissue-based consequences of APOL1 risk variants and expression. We tested associations between APOL1 risk alleles or glomerular APOL1 mRNA expression and 83 light- or electron-microscopy traits measuring structural and cellular kidney changes. Results: Under both recessive and dominant models in the FSGS patient subgroup (61%), APOL1 risk variants were significantly correlated (defined as FDR <0.1) with decreased global mesangial hypercellularity, decreased condensation of cytoskeleton, and increased tubular microcysts. No significant correlations were detected in MCD cohort. Independent of risk alleles, glomerular APOL1 expression in FSGS patients was not correlated with morphologic features. Conclusions: While APOL1-associated FSGS is associated with two risk alleles, both one and two risk alleles are associated with cellular/tissue changes in this study of FSGS patients. Our lack of discovery of a large group of tissue differences in FSGS and no significant difference in MCD may be due to the lack of power but also supports investigating whether machine learning methods may more sensitively detect APOL1-associated changes

An empirical determination of the whole-life cost of FO-based open-loop wastewater reclamation technologies

Over the past 5–10 years it has become apparent that the significant energy benefit provided by forward osmosis (FO) for desalination arises only when direct recovery of the permeate product from the solution used to transfer the water through the membrane (the draw solution) is obviated. These circumstances occur specifically when wastewater purification is combined with saline water desalination. It has been suggested that, for such an “open loop” system, the FO technology offers a lower-cost water reclamation option than the conventional process based on reverse osmosis (RO). An analysis is presented of the costs incurred by this combined treatment objective. Three process schemes are considered combining the FO or RO technologies with membrane bioreactors (MBRs): MBR-RO, MBR–FO–RO and osmotic MBR (OMBR)-RO. Calculation of the normalised net present value (NPV/permeate flow) proceeded through developing a series of empirical equations based on available individual capital and operating cost data. Cost curves (cost vs. flow capacity) were generated for each option using literature MBR and RO data, making appropriate assumptions regarding the design and operation of the novel FO and OMBR technologies. Calculations revealed the MBR–FO–RO and OMBR-RO schemes to respectively offer a ∼20% and ∼30% NPV benefit over the classical MBR-RO scheme at a permeate flow of 10,000 m3  d−1, provided the respective schemes are applied to high and low salinity wastewaters. Outcomes are highly sensitive to the FO or OMBR flux sustained: the relative NPV benefit (compared to the classical system) of the OMBR-RO scheme declined from 30% to ∼4% on halving the OMBR flux from a value of 6 L m−2. h−1.This work was made possible by the support of a National Priorities Research Programme (NPRP) grant from the Qatar National Research Fund ( QNRF ), grant reference number NPRP10-0118-170191 . The statements made herein are solely the responsibility of the authors. The authors would like to thank Dan Jerry Cortes from Qatar University and Arnold Janson from ConocoPhillips, Qatar for providing useful information for this paper.Scopu

Investigation of thin-film composite hollow fiber forward osmosis membrane for osmotic concentration: A pilot-scale study

The current study applied the forward osmosis (FO) based osmotic concentration (OC) process at the pilot-scale for concentrating synthetic feed solution (FS). The process water (PW) salinity represents effluents from the gas industry, while the draw solution (DS) mimics seawater. Besides, the performance of a hollow fiber (HF) membrane manufactured from polyamide thin film composite (PA-TFC) was evaluated. The effect of operation with various feed recovery rates, flowrates and temperatures on the OC performance was examined. Outcomes reveal that the tested membrane succeeded in recovering up to 90% of FS at water flux of 6.40 LMH. The stability of OC plant was successfully demonstrated for 48 hours long-term run at 75% feed recovery as an optimum condition, where the TFC membrane achieved average water flux of 6.00 LMH, respectively. Higher DS flowrate improved the OC performance by inducing higher water permeation and FS recovery; however, it increased the undesirable reverse solute diffusion. Lastly, the permeability coefficient of the HF membrane was estimated by 2.69 LMH/bar at 25 �C, which significantly enhanced at higher temperatures.This work was made possible by the support of a National Priorities Research Program (NPRP) grant from the Qatar National Research Fund (QNRF), grant reference number NPRP10-0118170191. The statements made herein are solely the responsibility of the authors. The authors would like to thank Dan Jerry Cortes from Qatar University and Arnold Janson from ConocoPhillips, Qatar for providing useful information for this paper.Scopu

Postoperative Continuous Passive Motion Does Not Improve the Range of Movement Achieved After Manipulation Under Anesthetic for Stiffness in Total Knee Replacement

Background Stiffness is a common complication following total knee arthroplasty. Manipulation under anesthesia (MUA) is an intervention that can potentially improve range of motion (ROM). Continuous passive motion (CPM) therapy has been utilized to enhance post-MUA ROM, but its effectiveness remains debated. This study assesses whether CPM therapy after MUA results in superior ROM outcomes compared to MUA alone. Methods A retrospective analysis included patients undergoing MUA for stiff primary total knee arthroplasty between 2017 and 2022. Demographics and ROM data were collected. Patients were in 2 groups: those who received inpatient CPM post-MUA and those who received day-case MUA alone. Complications and further interventions were noted. Results Of 126 patients, 39 underwent MUA only (day-case group), and 87 received CPM and MUA (inpatient group). Mean preoperative ROM was 69.4° (standard deviation [SD]:18.0°) and 73.9° (SD: 18.1°) for inpatient and day-case groups, respectively. Mean post-MUA ROM improved by 39.4° (SD: 17.7°) and 25.5° (SD: 11.1°) inpatient groups and day-case, respectively. The mean percentage of ROM gained at MUA maintained at final follow-up was 63.7% (40.8%) and 67.0% (47.5%) inpatient and day-case groups, respectively. Conclusions This study found no advantage in the routine use of CPM post-MUA for stiff total knee replacement patients, suggesting it may not provide sustained ROM improvements compared to MUA alone. Cost-effectiveness and patient selection merit further investigation