In vitro Activities of Amphotericin B, Itraconazole and Voriconazole against Isolates of Aspergillus spp

ABSTRACT This study was aimed at the drug susceptibility testing of Aspergillus fumigatus and Aspergillus flavus against amphotericin B, itraconazole and voriconazole using microdilution method proposed by National Committee for Clinical Laboratory Standards (NCCLS M38-P document). 50 isolates of Aspergillus spp. supplied from environment of Emam Khomeini hospital and minimum inhibitory concentrations (MIC) were determined after 48 h against mentioned drugs in accordance with NCCLS M38-P document.MICs results showed that voriconazole was more active drug than itraconazole with MICs between 0.5 to 1 µg /ml. The MICs ranges for itraconazole and amphotericin B were between 1-8 µg /ml and 0.5-2 µg /ml, respectively. Significant decrease in MICs was seen for voriconazole with respect to itraconazole (P < 0.05). Significant difference was not seen for each of drug in both A.fumigatus and A.flavus strains (P < 0.05). None of isolates showed in-vitro resistance to used drugs but for better judgment of drug resistance, clinical trials abreast of in-vitro results need to be established