Wreath determinants for group-subgroup pairs

The aim of the present paper is to generalize the notion of the group determinants for finite groups. For a finite group $G$ of order $kn$ and its subgroup $H$ of order $n$, one may define an $n$ by $kn$ matrix $X=(x_{hg^{-1}})_{h\in H,g\in G}$, where $x_g$ ($g\in G$) are indeterminates indexed by the elements in $G$. Then, we define an invariant $\Theta(G,H)$ for a given pair $(G,H)$ by the $k$-wreath determinant of the matrix $X$, where $k$ is the index of $H$ in $G$. The $k$-wreath determinant of $n$ by $kn$ matrix is a relative invariant of the left action by the general linear group of order $k$ and right action by the wreath product of two symmetric groups of order $k$ and $n$. Since the definition of $\Theta(G,H)$ is ordering-sensitive, representation theory of symmetric groups are naturally involved. In this paper, we treat abelian groups with a special choice of indeterminates and give various examples of non-abelian group-subgroup pairs.Comment: 12 pages, 2 figure

Zooplankton community structure influences the distribution of flying seabirds off Vincennes Bay, East Antarctica

The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

A new tool for monitoring the Antarctic marine ecosystem using environmental DNA

The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Participation of ezrin in bacterial uptake by trophoblast giant cells

<p>Abstract</p> <p>Background</p> <p>Trophoblast giant (TG) cells are involved in systematic removal of bacterial pathogens from the maternal-fetal interface of the placenta. In particular, TG cells have the ability to take up extracellular antigens by active phagocytosis induced by interferon-gamma (IFN-gamma). We previously reported that heat shock cognate protein 70 (Hsc70) present on the surface of TG cells mediated the uptake of Brucella abortus. However, the mechanism of bacterial uptake by TG cells is not completely understood. Here we identified ezrin, a member of ezrin-radixin-moesin (ERM) protein family, as a molecule associated with Hsc70.</p> <p>Methods</p> <p>Mouse TG cells were employed in all experiments, and B. abortus was used as the bacterial antigen. Confirmation of the binding capacity of ERM protein was assessed by pull-down assay and ELISA using recombinant Hsc70 and ERM proteins. Ezrin was depleted using siRNA and the depletion examined by immunoblotting or immunofluorescence staining.</p> <p>Results</p> <p>The expression level of ezrin was higher in TG cells than in trophoblast stem (TS) cells, and ezrin knockdown TG cells showed a reduction in bacterial uptake ability. Although tyrosine phosphorylation of ezrin was not related to bacterial uptake activity, localization of Hsc70 on the membrane was affected by the depletion of ezrin in TG cells.</p> <p>Conclusion</p> <p>Ezrin associates with Hsc70 that locates on the membrane of TG cells and participates in the bacterial uptake by TG cells.</p

EEVD motif of heat shock cognate protein 70 contributes to bacterial uptake by trophoblast giant cells

<p>Abstract</p> <p>Background</p> <p>The uptake of abortion-inducing pathogens by trophoblast giant (TG) cells is a key event in infectious abortion. However, little is known about phagocytic functions of TG cells against the pathogens. Here we show that heat shock cognate protein 70 (Hsc70) contributes to bacterial uptake by TG cells and the EEVD motif of Hsc70 plays an important role in this.</p> <p>Methods</p> <p><it>Brucella abortus </it>and <it>Listeria monocytogenes </it>were used as the bacterial antigen in this study. Recombinant proteins containing tetratricopeptide repeat (TPR) domains were constructed and confirmation of the binding capacity to Hsc70 was assessed by ELISA. The recombinant TPR proteins were used for investigation of the effect of TPR proteins on bacterial uptake by TG cells and on pregnancy in mice.</p> <p>Results</p> <p>The monoclonal antibody that inhibits bacterial uptake by TG cells reacted with the EEVD motif of Hsc70. Bacterial TPR proteins bound to the C-terminal of Hsc70 through its EEVD motif and this binding inhibited bacterial uptake by TG cells. Infectious abortion was also prevented by blocking the EEVD motif of Hsc70.</p> <p>Conclusions</p> <p>Our results demonstrate that surface located Hsc70 on TG cells mediates the uptake of pathogenic bacteria and proteins containing the TPR domain inhibit the function of Hsc70 by binding to its EEVD motif. These molecules may be useful in the development of methods for preventing infectious abortion.</p

Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion

<p>Abstract</p> <p>Background</p> <p>The cell tropism of <it>Brucella abortus</it>, a causative agent of brucellosis and facultative intracellular pathogen, in the placenta is thought to be a key event of infectious abortion, although the molecular mechanism for this is largely unknown. There is a higher degree of bacterial colonization in the placenta than in other organs and many bacteria are detected in trophoblast giant (TG) cells in the placenta. In the present study, we investigated mechanism of <it>B. abortus </it>invasion into TG cells.</p> <p>Results</p> <p>We observed internalization and intracellular growth of <it>B. abortus </it>in cultured TG cells. A monoclonal antibody that inhibits bacterial internalization was isolated and this reacted with heat shock cognate protein 70 (Hsc70). Depletion and over expression of Hsc70 in TG cells inhibited and promoted bacterial internalization, respectively. IFN-γ receptor was expressed in TG cells and IFN-γ treatment enhanced the uptake of bacteria by TG cells. Administering the anti-Hsc70 antibody to pregnant mice served to prevent infectious abortion.</p> <p>Conclusion</p> <p><it>B. abortus </it>infection of TG cells in placenta is mediated by Hsc70, and that such infection leads to infectious abortion.</p

Locating Congested Segments over the Internet Based on Multiple End-to-End Path Measurements

Since congestion is very likely to happen in the Internet, locating congested areas (path segments) along a congested path is vital to appropriate actions by Internet Service Providers to mitigate or prevent network performance degradation. We propose a practical method to locate congested segments by actively measuring one-way end-to-end packet losses on appropriate paths from multiple origins to multiple destinations, using a network tomographic approach. Then we conduct a long-term experiment measuring packet losses on multiple paths over the Japanese commercial Internet. The experimental results indicate that the proposed method is able to precisely locate congested segments. Some findings on congestion over the Japan Internet are also given based on the experimen

Ontogenetic changes in food habits of larval and juvenile Antarctic myctophids, Electrona antarctica, in the north of Lützow-Holm Bay, East Antarctica

第3回極域科学シンポジウム/第34回極域生物シンポジウム 11月26日（月） 国立極地研究所 ３階ラウン

High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells

Metallothionein (MT) is an intracellular metal-binding, cysteine-rich protein, and is a potent antioxidant that protects cells and tissues from oxidative stress. Although the major isoforms MT-1 and -2 (MT-1/-2) are highly inducible in many tissues, the distribution and role of MT-1/-2 in diabetic nephropathy are poorly understood. In this study, diabetes was induced in adult male rats by streptozotocin, and renal tissues were stained with antibodies for MT-1/-2. MT-1/-2 expression was also evaluated in mProx24 cells, a mouse renal proximal tubular epithelial cell line, stimulated with high glucose medium and pretreated with the antioxidant vitamin E. MT-1/-2 expression was gradually and dramatically increased, mainly in the proximal tubular epithelial cells and to a lesser extent in the podocytes in diabetic rats, but was hardly observed in control rats. MT-1/-2 expression was also increased by high glucose stimulation in mProx24 cells. Because the induction of MT was suppressed by pretreatment with vitamin E, the expression of MT-1/-2 is induced, at least in part, by high glucose-induced oxidative stress. These observations suggest that MT-1/-2 is induced in renal proximal tubular epithelial cells as an antioxidant to protect the kidney from oxidative stress, and may offer a novel therapeutic target against diabetic nephropathy