Invasive ductal carcinoma ex pleomorphic adenoma of the lacrimal gland - a long term follow-up case

We experienced a case of malignant orbital tumor in a 72-year-old Japanese man. He noticed protrusion of his left eye, and received resection of the left orbital tumor. The tumor contained several different components such as high-grade invasive ductal carcinoma, low-grade/minimally invasive adenocarcinoma and pleomorphic adenoma. Pathological diagnosis of high-grade invasive ductal carcinoma ex pleomorphic adenoma of the left lacrimal gland was made. He had undergone surgical removal of the left lacrimal gland tumor at the other hospital 19 years before, and the surgical specimen revealed that most part of the tumor was pleomorphic adenoma but low-grade/minimally invasive adenocarcinoma was included as a minor component (low-grade/minimally invasive adenocarcinoma ex pleomorphic adenoma). Thus, we could observe the natural course of low-grade/minimally invasive adenocarcinoma ex pleomorphic adenoma which transformed into high-grade invasive ductal carcinoma ex pleomorphic adenoma. Lacrimal gland ductal carcinoma is an extremely rare tumor, and only 25 cases have been reported before in English literature. Moreover, our case is the 5th case of lacrimal gland ductal carcinoma ex pleomorphic adenoma. Keywords: Lacrimal gland, Carcinoma ex pleomorphic adenoma, Androgen receptor, HER

Infrared venography of the hand in Apert syndrome

As well as craniofacial synostosis, complex syndactyly of hands is a distinctive feature of Apert syndrome. Consideration of blood flow to the digits is very important in separation surgery. Several reports offer information about arterial distribution in Apert′s hands. Though, venous pattern has not been well discussed. Infrared venography offers a real-time image with minimal invasion. An Apert syndrome patient underwent a series of finger splitting surgeries. Infrared venography was carried out to assess veins. There was a palmar venous arch, placing distally to the metacarpophalangeal joint. The arch had to be cut to divide fused fingers sufficiently. As well as arterial abnormality, venous uniqueness should be noted in Apert syndactyly surgeries. Infrared venography, which can be carried out easily, offers good information that surgeon require

Tracking of implanted cells.

<p>DiI-labeled cells were fluorescently identified (red) by CD31/PECAM1 in sections stained with DAB and counterstained with hematoxylin. Objective lens: 40x; bar: 50 micro meters. pb: pb-PRP injected, bm: bm-PRP injected, f: fluorescent view, m: merged with bright-field view. A: 2 weeks after injection. In both pb and bm, fluorescence was identified in wound bed muscular layer. B: 4 weeks after injection. In pb, fluorescence was hardly observed, whereas in bm, robust fluorescence was confirmed. Correlation between fluorescence and DAB stain was unclear.</p

A new rabbit model of impaired wound healing in an X-ray-irradiated field - Fig 1

<p>A: Schematic diagram of shielding. Irradiation was restricted to a hindlimb. B: An anesthetized rabbit in a shielding apparatus was placed in an X-ray irradiation unit.</p

Histological view of the wounds 2 weeks after the surgery.

<p>Serial sections were prepared and stained; 2x objective lens; bar: 1 mm. R-: without irradiation, PBS injected. R+: irradiated, PBS injected. pb: irradiated, peripheral blood-derived PRP injected. bm: irradiated, bone marrow aspirate-derived PRP injected. HE: hematoxylin-eosin stain. CD31: DAB stained with anti-CD31/PECAM1 antibody, counterstained with hematoxylin. PR: Picrosirius red stain, bright-field view. PP: Picrosirius red stain, polarized view. Black triangles in first row (HE) indicate epidermal edges.</p

Percentage of colored areas in picrosirius red-stained, polarized images of normal and post-irradiated skin.

<p>Percentage of colored areas in picrosirius red-stained, polarized images of normal and post-irradiated skin.</p

Picrosirius red staining just below epidermal defect.

<p>Four weeks after wound creation; 20x objective lens; bar: 100 micro meters. R-: without irradiation, PBS injected. R+: irradiated, PBS injected. pb: irradiated, peripheral blood-derived PRP injected. bm: irradiated, bone marrow aspirate-derived PRP injected. PR: Picrosirius red stain, bright-field view. PP: Picrosirius red stain, polarized view. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184534#pone.0184534.t002" target="_blank">Table 2</a>.</p

Percentage of CD31/PECAM1-positive area in neoplastic granulation tissue.

<p>Percentage of CD31/PECAM1-positive area in neoplastic granulation tissue.</p