THE ROLE OF PROPOLIS TETRAGONULA SP. IN OXIDATIVE STRESS AND ITS PROTECTIVE EFFECT AGAINST UV RADIATION ON CELLS

Objective: This report presents a study the anti-oxidant properties of propolis wax from Tetragonula sp. bees by evaluating the protection ability of propolis towards cells from ultraviolet (UV) exposure. The next evaluation was using a literature study and LC-MS (Liquid Chromatography-Mass Spectrometry) to find compounds in propolis that are responsible for anti-oxidative stress activity. Methods: HEK 293T (Human Embryonic Kidney 293 T) and fibroblast cells line were used. Four tests were performed on the cells, namely: cell proliferation assay using water-soluble tetrazolium salt (WST-8); lactate dehydrogenase (LDH), and free radicals produced on cells test by measuring fluorescence intensity produced by dichlorofluorescein; cell viability through observation using fluorescence microscope on cells stained with Hoechst and propidium iodide (PI); and reactive oxygen assay (ROS) Assay. Before UV exposure, propolis wax was added to the cells in different concentrations. The authors also analyzed the component in propolis wax using LC-MS. Results: Based on this assessment, it was found that propolis wax successfully protects the cells against UV-induced free radicals’ formation by maintaining the cell proliferation rate, reducing the free radicals produced after UV exposure, and decreasing the number of cell death. Nevertheless, we found that a greater concentration of propolis wax tends to be toxic to the cells. While on the LC-MS results obtained about 83 compounds, in which 35 of them are flavonoid and polyphenols derived compounds with antioxidant properties. Conclusion: Based on these findings, propolis wax produced Tetragonula sp. can be used as a potential alternative treatment of anti-oxidative stress and anti-free radicals

Abstracts of the International Halal Science Conference 2023

This book presents the extended abstracts of the selected contributions to the International Halal Science Conference, held on 22-23 August 2023 by the International Institute for Halal Research and Training (INHART), IIUM, Malaysia in collaboration with Halalan Thayyiban Research Centre, University Islam Sultan Sharif (UNISSA), Brunei Darussalam. With the increasing global interest in halal products and services, this conference is timely. Conference Title:  International Halal Science ConferenceConference Acronym: IHASC23Conference Theme: Halal Industry Sustainability Through ScienceConference Date: 22-23 August 2023Conference Venue: International Islamic University (IIUM), MalaysiaConference Organizer: International Institute for Halal Research and Training (INHART), International Islamic University (IIUM), Malaysi