A Critical Role of Fatty Acid Binding Protein 4 and 5 (FABP4/5) in the Systemic Response to Fasting

学位記番号：医博甲148

Moringa oleifera Lam. to accelerate wound healing: a review

An injury to the skin that disrupts the soft tissue may form a wound. The healing process in response to injury is a dynamic and well-regulated process of cellular, humoral, and molecular mechanisms that consists of four partly overlapping phases: hemostasis, inflammation, proliferation, and remodeling. An impaired wound-healing process may cause a formation of an abnormal scar and chronic wounds, leading to a reduced life quality. Therefore, it needs an optimal prevention strategy. Many modalities have been claimed to accelerate wound healing. The trend of using natural products is increasing in most Southeast Asian countries due to their biodiversity. Nowadays, studies on natural compounds are increasing to accelerate wound healing. Moringa oleifera Lam. is a high-value plant that each part of it has a high nutritional value as well as a great range of medicinal uses, including anti-inflammatory, antimicrobial, antioxidant, and wound healing properties. In this review, we have explored the M. oleifera that are very rich in vitamins, minerals, fatty acids, and phytochemical compounds like quercetin, kaempferol, and vicenin-2, that play a role in the wound healing process. Moreover, these compounds may enhance the healing of wounds with pathological conditions such as diabetes, immunocompromised and persistent infection

Literature Study of The Potential of Moringa Oleifera Leaves Powder Supplementation to Enhance The Coloration of Ornamental Fish

Efforts to maintain good ornamental fish are by providing the quality of feed. The quality of feed is assessed from the best nutritional content as well as economical and can increase the brightness of ornamental fish. Feeds with pigment carotenoids complexes are known to be the main sources of ornamental fish skin pigmentation. A literature study was conducted to explore the benefits of Moringa oleifera leaves powder to improve the color of ornamental fish through carotenoid content. Based on the results of literature studies, supplementation of these natural products has potentially elevated the color brightness of ornamental fish at carotenoid dose 520 mg/kg. Further research on the potential and formulation of M. oleifera leaves powder supplementation impact color brightness of ornamental fish in vivo is very important to explain the mechanism of carotenoids in influencing the physical color brightness of ornamental fis

Viabilitas Oosit Pasca Vitrifikasi menggunakan Kombinasi Ethilen Glikol dan Dimetil Sulfoksida dengan Dua Level Konsentrasi yang Berbeda

Vitrifikasi merupakan metode kriopreservasi untuk membekukan sel secara cepat, tanpa disertai terbentuknya kristal es. Vitrifikasi dilakukan dengan menggunakan krioprotektan yang memiliki toksitas. rendah sehingga oosit dapat mempertahankan viabilitasnya. Dimetil sulfoksida (DMSO) dan ethylene glycol (EG) merupakan krioprotektan intraseluler dengan toksisitas rendah sehingga kombinasi kedua krioprotektan tersebut diharapkan dapat meningkatkan viabilitas oosit pasca vitrifikasi. Tujuan dari penelitian ini adalah untuk mengevaluasi viabilitas oosit pasca vitrifikasi dengan menggunakan kombinasi dan DMSO dan EG pada konsentrasi yang berbeda. Penelitian dilaksanakan di Laboratorium Riset dan Bioteknologi, Fakultas Peternakan, Universitas Padjadjaran periode September 2016-Desember 2016. Penelitian menggunakan Rancangan Acak Lengkap dengan dua kelompok perlakuan,  yaitu media vitrifikasi dengan dua konsentrasi yang berbeda: 15% DMSO+15% EG dan media 17% DMSO+17%EG. Setelah seminggu penyimpanan, maka dilakukan proses warming untuk mengevaluasi viabiltias oosit pasca vitrifikasi. Hasil menunjukkan bahwa viabilitas oosit yang divitrifikasi dengan menggunakan 17% DMSO+17%EG nyata lebih tinggi apabila dibandingkan dengan 15% DMSO+15%EG. Kata kunci: vitrifikasi, Dimetil sulfoksida, Ethylen glikol, viabilitas oosit

Perbandingan Viabilitas Oosit Domba Pasca Vitrifikasi dengan Menggunakan Hemistraw dan Cryotop

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengkaji efek vitrifikasi dengan menggunakan dua buah system carrier yang berbeda terhadap viabilitas oosit domba yang telah dimaturasi secara in vitro. Oo­sit dibagi menjadi dua kelompok perlakuan, yaitu (i) divitrifikasi dengan menggunakan hemistraw (ii) divitrifikasi dengan menggunakan cryotop. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vi­trifikasi serupa pada kedua jenis carrier yang digu­nakan untuk vitrifikasi. Oosit diletakkan dalam larutan equilibrasi yang mengandung konsentrasi permeable kriopro­tektan setengah dari larutan vitrifikasi. Se­telah 15 menit, oosit ditransfer ke dalam media vitrifikasi yang mengandung 17% EG+17% DMSO +0, 65M sukrosa di dalam modified PBS yang dis­uplementasi dengan 20% fetal bovine serum. Total waktu yang digunakan untuk memaparkan oosit ke dalam laru­tan vitrifikasi adalah 30 detik. 5-8 oosit dipipet menggunakan kapiler gelas dan diletak­kan/loading ke dalam carrier yang digunakan (hemistraw atau cryotop) kemudian langsung di paparkan ke dalam nitrogen cair. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vi­trifikasi serupa pada kedua jenis carrier yang digu­nakan untuk vitrifikasi(Comparation of sheep oocyte viability after vitrification using hemistraw and cryotop)ABSTRACT. The aim of this study was to examine the effect vitrification using two different carrier system on the matured sheep oocytes viability. Oocytes were devided into two group (i) vitrified using hemistraw (ii) vitrified using cryotop. Oocytes placed into equilibration solution which is containing a half concentration permeable cryoprotectant of vitrification solution. After 15 minute, oocytes were transferred into vitri­fication solution containing 17% EG+17% DMSO +0, 65M sucrose in modified PBS supplemented with 20% fetal bovine serum. The total exposure time of oocytes to vitrification solution was 30 sec. Oocytes were pipetted into a glass capillary into group 5-8, and loaded into carrier (hemistraw or cryotop) then plugged into liquid nitrogen. After a week cryopreservation, oocytes were warmed and cultured in TCM 199 suplemented with 10% fetal bovine serum at 38.50 C under 5% CO2 for 3h. Oo­cytes viability was evaluated by re expansion, color and homogeneity of oocyte cytoplasm. Our results indicated that the oocytes viability after vitrification was similar from both of carrier for vitrificatio

THE HEMATOLOGIC PROFILE IN THE ACUTE TOXICITY TEST OF COGON GRASS ROOTS ETHANOL EXTRACT IN WISTAR RATS

Objective: This study aimed to investigate the hematologic profile of Wistar rats in the acute toxicity test of Cogon grass roots ethanol extract (CGEE). Methods: Cogon grass roots were dissolved in 70% ethanol. An acute toxicity test was conducted based on The National Agency of Drug and Food Control of the Republic of Indonesia. Five female rats in the treatment group were administered a single high dose of 5000 mg/kg body weight (BW) of CGEE in 200 μl of 0.5% carboxymethyl cellulose (CMC), and the 5 female rats in the control group were administered 200 μl of 0.5% CMC. After 14 d, blood samples were collected, and 18 hematologic parameters were measured with a hematology analyzer. Statistical analyses were performed to compare the parameters between the two groups with the independent t-test for normally distributed data and the Mann Whitney test for non-normally distributed data. Results: None of the hematologic parameters in the treatment group significantly differed from those in the control group after 14 d of observation (P>0.05). Conclusion: A single high dose of 5000 mg/kg BW of CGEE did not change the hematologic profile of Wistar rats. These results indicate that CGEE does not have an acute hemotoxic effect, at least for hematologic parameters

Effect of Moringa Leave Ethanol Extract on Accelerating Wound Healing Process

TGF-β and VEGF are vital in cell proliferation and regeneration, as evidenced in processes like wound healing. The leaves of Moringa oleifera Lam exhibit anti-inflammatory and cell regenerative properties that may facilitate the transition from the inflammatory to the proliferative phase, enhancing wound repair. This research sought to discern the potential of orally administered moringa leaf extract in augmenting systemic wound healing, focusing on TGF-β and VEGF serum as in vivo molecular markers. This research was conducted at the Animal Laboratory of the Faculty of Medicine, Universitas Padjadjaran, and the Laboratory of Molecular Genetics, Universitas Padjadjaran, from January to March 2022. We divided thirty Swiss Webster mice into two categories: healthy and wound-treated. Wounded mice received 100 mg/kgBW Na CMC as a negative control, 500 mg/kgBW zinc syrup as a positive control, and M. oleifera leaves ethanol extract (MOLE) in doses of 280 and 560 mg/kgBW orally from day 3 to day 6. Wound progression was documented and measured on days 0, -1, -3, and -6. Day-6 blood samples were obtained, and TGF-β and VEGF serum levels were gauged using ELISA. Results from day 6 revealed that wound coverage in the 280 and 560 mg/kgBW MOLE groups was 13.76±5% and 13.38±4%, respectively. These percentages notably surpassed that of the negative control group (p=0.005). However, the TGF-β and VEGF serum levels in the MOLE-treated groups did not differ significantly from the negative control (p=0.081 and p=0.149, respectively). Thus, the study concludes that while MOLE expedites wound closure, it does so without the systemic involvement of TGF-β and VEGF in vivo

Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method

Objective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxygen species (ROS) that reduces sperm quality.Methods: Semen samples that were collected from 31 male partners of couples undergoing infertility investigation were analyzed for sperm concentration, motility, and leucocytes concentration. Semen samples were then divided in two groups based on their leucocytes concentrations (category A: >0 to 1 x 106/mL. Semen samples were processed using density-gradient centrifugation technique. Results: There was a significant difference in the number of sperms harvested and sperm motility after preparation. Interestingly, pregnancy rate after IUI was higher (p<0.05) in non-leukocytospermia semen (39%) when compared to leukocytospermia semen (30%).Conclusions: Seminal leukocytes (PMNL) concentration affects pregnancy rate after intrauterine insemination. Keywords: Density gradient method, sperm recovery rate, intrauterine insemination, pregnancy rate DOI: 10.15850/ijihs.v6n2.131

IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5° C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming

Oral Administration of Cogongrass (Imperata cylindrica L) Root Ethanol- Extract causes Mouse Epididymal Sperm Abnormality (PEMBERIAN EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICA L) SECARA ORAL MENYEBABKAN ABNORMALITAS SPERMA EPIDIDYMIS MENCIT)

Sperm morphology is an important parameter to be observed in the male fertility. Some of the bioactive compounds of cogongrass root such as alkaloid and terpenoid, affect male fertility by interference the spermatogenesis. The objective of the study was to observe the effect of cogongrass root ethanol extract on mouse sperm morphology. This study was carried out by oral administration of two different doses i.e 90 and 115 mg/kg body weight of cogongrass root ethanol extract into 8-10 weeks old DDY strain mice for 14 days to evaluated the acute effect due to the administration of cogongrass root ethanol extract on mouse sperm morphology. The results showed that treatment with cogongrass root ethanol extract significantly increased sperm abnormalities followed a dose depending pattern (p<0.05). Interestingly, the administration of cogongrass root extract did not affect sperm head morphology but tailless, folded and bent sperm increased linearly with the administration dose of cogongrass root ethanol extract. In conclusion, cogongrass root ethanol extract causes  secondary sperm abnormalitties on mouse sperm