Ex Vivo and In Vivo Retention Time Evaluation of Fucoidan Isolated from Macrocystis pyrifera Through a Thermosensitive Gel System in The Vaginal Route

This study evaluated Fucoidan from Macrocystis pyrifera as a potential treatment for cervical cancer. The research aimed to examine Fucoidan’s in vivo retention capacities in poloxamer-based in situ gels for vaginal drug delivery systems. Five different thermosensitive gel formulations were developed, each with varying concentrations of Pluronic F127 and F68 polymers. The incorporation of HPMC affected the gelation temperature, viscosity, and bioadhesive strength. The accepted formula, F3, had a bioadhesive value of 5415.93 ± 98.74 dyne/cm2 and could form a gel at physiological temperature. Ex vivo animal models showed that Fucoidan components retained well on vaginal tissue. Only F1, F2, and F3 achieved the media after 8 hours of examination. In vivo evaluation showed F3 had the highest drug concentration retained in the vaginal mucosa of female rats after 8 hours (24,115 ± 4,842 g), slowly removed after 24 hours (13,014 ± 5,596 g). In conclusion, increases in the hydrophilic content of formulations led to the retained hydrogel formula, which increased drug release and lowered intravaginal elimination

The Cardioprotective Effect of Polysaccharide Sulphate Isolated from Brown Algae (Sargassum polycystum)

The incidence of atherosclerosis is characterized by an increase in the value of low-density lipoprotein (LDL) and a decrease in the value of high-density lipoprotein (HDL) as well as an increase in the total white blood cell count which can indicate the occurrence of atherosclerosis. This study used 18 rats which were divided into 6 groups of 3 each, namely a normal control group, a negative control group (CMC 0.5%), a positive control group (Simvastatin 20 mg/kg BW), and 3 groups given a sulfate polysaccharide isolate compound test material (dosage of 250, 50, and 10 mg/kg of body weight). The results showed that sulfated polysaccharide isolates had an effect in reducing white blood cells significantly between doses of 250 mg/kg BW and 50 mg/kg BW as well as reducing SGOT levels. Unfortunately it did not reduce the SGPT level. The results of the Mann-Whitney post hoc test showed that administration of sulfated polysaccharides at an optimal dose of 250 mg/kg BW reduced the number of foam cells in the atherosclerotic white rats' (Rattus norvegicus) aortas that were given a high-fat diet and had activity in reducing CKMB levels compared to other doses