BMJOpen_SuppTable2

Educational strategies used in published studies describing pediatric surgery checklist implementatio

BMJOpen_SuppTable3

Attitudes across pediatric surgical teams in regards to the surgical safety checklis

Timeline for experimental procedures.

<p>Rats were kept NPO from one day before surgery until the end of experimentation, and maintained on TPN for 7 d after surgery for bowel transection or resection (A). GLP-2 rats were given recombinant human GLP-2 (1–33) mixed into the daily volume of TPN as a continuous infusion (2.5 nmol/kg/h). Fat, protein and carbohydrate content in TPN were advanced step-wise to reach 100% of daily calories (250 kcal/kg/d) by POD 2 (B). Abbreviations: <i>nil per os</i> (<b>NPO</b>), post-operative day (<b>POD</b>).</p

Plasma hormone and glucose concentrations.

<p>Rats were subjected to bowel transection or resection, then maintained with TPN for 7 d with or without exogenous GLP-2. The fasting plasma concentrations include GLP-2 (A), glucose (B), GLP-1 (C), insulin (D), PYY (E), and amylin (F). Values are means±SD. Labelled means with different superscripts were significantly different (<i>P</i> < 0.05). TB (n = 6–7), TB GLP-2 (n = 7), SBS (n = 3–5), SBS GLP-2 (n = 5–7).</p

Target and endogenous control gene primer sequences and annealing temperatures for qPCR.

<p>Target and endogenous control gene primer sequences and annealing temperatures for qPCR.</p

Jejunal hexose transporter and GLP-2R transcript abundance.

<p>Rats were subjected to bowel transection or resection, then maintained with TPN for 7 d with or without exogenous GLP-2. SGLT-1 (A), GLUT-2 (B), GLUT-5 (C) and GLP-2R (D) mRNA abundance by qPCR. Data were expressed as relative fold changes using 18S ribosomal RNA as the endogenous control. Values are means, with error bars representing the upper and lower limits. Labelled means with different superscripts were significantly different (<i>P</i> < 0.05). TB (n = 7), TB GLP-2 (n = 7), SBS (n = 5), SBS GLP-2 (n = 7).</p

Whole mount immunohistochemistry.

<p>Rats were subjected to bowel transection or resection, then maintained with TPN for 7 d with or without exogenous GLP-2. Proportion of nNOS staining neurons (number of nNOS positive neurons / total HuC/D neuronal nuclear stained neurons per ganglia) in jejunal (A) and colonic (B) myenteric plexuses. Representative whole-mount immunohistochemistry for anti-HuC/D (neuronal nuclei stain in green) and anti-nNOS (perinuclear stain in red) at 20X magnification (C). Values are means±SD. Labelled means with different superscripts were significantly different (<i>P</i> < 0.05). TB (n = 4), TB GLP-2 (n = 4), SBS (n = 5), SBS GLP-2 (n = 5).</p

Significance of main effects and interactions of resection with GLP-2 on all endpoints depicted in figures.

<p>Significance of main effects and interactions of resection with GLP-2 on all endpoints depicted in figures.</p