Tadalafil as an agent of pharmacological preconditioning in ischemic - reperfusion brain injury

Ischemic stroke or cerebral infarction is the main pathology among severe forms of vascular lesions of the brain. One of the more effective non-medicamental methods of treatment is pharmacological preconditioning. Pharmacological neuroprotection is one of the treatment areas to reduce the damage in ischemic stroke and other modifications of brain ischemia. Therefore, the development and introduction of new pharmacological agents that can reduce the degree of ischemic-reperfusion injury of the brain, remains one of the major challenges of modern medicine. The most promising to explore, from our point of view, is a PDE-5 inhibito

Correction of adma-induced preeclampsia with the use of phosphodiesterase 5 and selective inhibitor of arginase II ZB49-0010

Simulation experimental ADMA-like preeclampsia was carried out by administering the rats with L-NAME during 14-20 days of pregnancy. In animals, there was an increase in blood pressure, proteinuria, impaired microcirculation in the placenta, the violation of the regulation of vascular tone and destructive changes in the ischemic placenta. The use of phosphodiesterase 5 and selective inhibitor of arginase II ZB49-0010 leads to the expression of morphological and functional correction of violations occurring in modeling of experimental preeclampsi

Vermicompost effect on the trace elements distribution in the luvic chernozem of the Krasnoyarsk forest-steppe

n the field experiment (2016-2018) in the conditions of the Krasnoyarsk forest-steppe the number of trace elements mobile forms were determined and the effect of soil organic matter and increasing doses of vermicompost on their distribution in luvic chernozem was studied. It is established that the studied vermicompost does not pollute the soil, which allows its widespread use in agriculture. The correlation and regression analysis obtained linear regression equations that reflect the relationship of the chemical element and organic matter in the soil after using increasing doses of vermicompost adequately. Elements with positive correlation and high significance level include Mn, Cu, Co and Zn

Simulation of total brain ischemia in rats

This paper deals with the study of the dependence of psycho neurological deficit in rats on the severity of brain damage, conducted on the four-vascular models of total cerebral ischemia. The obtained results demonstrate a tendency to deterioration of neuropsychiatric status of the animals with increasing severity of ischemic brain damage that allows suggesting the severity of ischemic brain damage and its correction by the degree of behavioral disorder

Neuroprotective effects of the novel ethylthiadiazole derivatives (LHT 4-15) in male rats

The study was performed on male rats of the Wistar line. The animals were simulated with total cerebral ischemia with preliminary administration of LHT 4-15 compounds in doses of 25 and 50 mg/kg for 60 min. The data obtained from the neurological deficit and in the behavioral tests of the experimental groups confirm the theory of the presence of ethylthiadiazole derivatives under the LHT code 4–15 neuroprotective properti

Ion channel TRPA₁ is a promising therapeutic target for treatment of pain

In the last time information about the role of TRPA₁ in pain and cold sensitivity, as well as in the formation and maintenance of inflammation is increasing in scientific literature. Given this information, the interest for search and study of pharmacological agents, which selectively blocked of TRPA₁ and reduced the severity of pain and inflammation is increasin

Improved delivery of plasmid DNA complexes with polycation into human cells in the presence of block copolymers of ethylene oxide and propylene oxide

The work is aimed at the study of the effect of novel block copolymers of ethylene oxide and propylene oxide on the delivery of plasmid DNA and its complexes with cationic polymers into human cells. Tri-functional amphiphilic block copolymers on the basis of glycerol (LaprolsTM), polyethyleneimine (25 kDa) and commercial transfection reagent TurboFectTM were tested as delivery systems. TurboFect was found to form more compact and positively charged polyplexes with plasmid DNA (pEGFP-N2) and provided higher expression of GFP in HEK 293 cells compared to polyethyleneimine. Laprols weakly interacted with plasmid DNA and did not improve its intracellular delivery. However they markedly promoted cell transfection by DNA-polyethyleneimine complexes. Results show that Laprols exhibit mild cytotoxicity and produce the interest for gene therapeutics delivery into cells

Thymosin β4 as basis for creation of a reparation preparation of new generation

On the basis of its multipurpose activities during regeneration of fabrics in various experiments on animals, Thymosin β4 has the potential for new researches, in kidneys and a liver, and also recovery of a spinal cord, bones and injury of ligaments. Besides, it can be useful in case of treatment of a wide range of other diseases, including concerning consequences of old bacterial damages and viral infection