9 research outputs found
A novel duplex ACRS-PCR for composite CSN1S1âCSN3 genotype discrimination in domestic buffalo
Quali sono gli aspetti prioritari nella professione infermieristica? Percezione degli infermieri delle sue prioritĂ , di quelle aziendali e di quelle del paziente
Un indicatore di benessere dellâinfermiere, puĂČ essere costituito dalla sua percezione di
condivisione con lâorganizzazione in cui lavora e degli utenti alla cui assistenza Ăš
deputato, del valore attribuito ai vari aspetti della sua professione e dei bisogni ad essa
associati.
In unâindagine empirica pilota sono state indagate la percezione di condivisione fra
infermiere, organizzazione ed utenti del valore attribuito ai vari aspetti della professione.
I soggetti sono 66 infermieri operanti in un grande ospedale romano, reclutati su base
volontaria.
Gli interrogativi proposti riguardavano i tre aspetti considerati dagli intervistati come
prioritari nel loro lavoro, quelli che secondo la loro esperienza, fossero considerati
prioritari dalla loro direzione organizzativa, e quelli ritenuti da loro prioritari, per lâutente.
Successivamente Ăš stato proposto un questionario di Martorello âLa mia soddisfazione
professionale e le proposte dellâaziendaâ riguardo alle seguenti aree: tecnologia, incentivazione
economica, flessibilitĂ e formazione.
Ă stata riscontrata una discordanza significativa, nella percezione degli infermieri
interpellati, tra le prioritĂ lavorative per gli infermieri, relative soprattutto alla
competenza professionale, quelle ritenute prioritarie per lâorganizzazione, relative alla
mera presenza in servizio e quelle ritenute prioritarie per i pazienti, relative soprattutto
alla relazione dâaiuto
A novel duplex ACRS-PCR for composite CSN1S1-CSN3 genotype discrimination in domestic buffalo
This short communication aimed to develop a fast and straightforward method for the simultaneous
discrimination in buffaloes of the alleles CSN1S1 A/B and CSN3 X1/X2 using a single protocol.
DNA was isolated from 219 individual blood samples. A duplex artificially created restriction
site (ACRS-PCR) was accomplished using two pairs of primers generating 86 bp (CSN1S1) and
160 bp (CSN3). Amplicons were contemporary digested by MboI and HinfI for the identification
of genotypes.
The double simultaneous amplification and digestion proved to be effective for allele identification.
The method resulted particularly quick due to the small PCR amplicons and fast digest
enzymes that allowed both a rapid amplification (about 1 h and 40 min) and digestion in
10 min. Population analysis indicated that minor allele frequencies were CSN1S1 A (0.425) and
CSN3 X2 (0.306). Linkage disequilibrium (LD) showed r2Œ0.46, and haplotype analysis revealed
all four possible combinations with higher frequency for the CSN1S1 B-CSN3 X1 (0.553).
Considering the tremendous economic impact of the CSN1S1âCSN3 variants on the dairy production
in buffalo, this method, applicable immediately after the birth from any DNA source,
may speed up the selection of sires and damsâ lines with more favourable genotype
Artidactyla retroposons detection at the Mediterranean river buffalo CSN1S2 gene
The αs2-casein is an important phosphoprotein secreted in ruminantsâ milk but, nowadays, the encoding gene (CSN1S2) has not
been characterized in buffaloes. In this study, we report the first
characterization of buffalo CSN1S2 gene and its artiodactyla retroposons (RTs) distribution. For this purpose, we amplified and
sequenced the whole gene of 2 Mediterranean river subjects
eared in Campania (Italy). The gene includes 1025 bp of coding
(18 exons) and 17578bp of intronic regions, 937 bp at the 5â-end
and 707 bp at the 3â-end and it is characterized by 13 RTs. The
first (named A) is located in the promoter region and appears to
be a Bov-tA2. Further 2 elements are located in intron 1 (B and
C) and show a strong similarity with L1_Art sequence. Two
Bov-tA2 are located in the intron 2 (D and E). The intron eight
includes a Bov-B (F), whereas the intron 12 contains a Bov-A2
(G). Five additional retroposons (H, I, L, M, N) are located in the
intron 13 (Bov-tA1), 15 (Bov-A2) and 17 (Bov-tA1, Bov-B, Bov-A2).
Finally, a Bov-tA2 (O) is located in the 3â UT, near to the last exon.
The observed RTs represent 19.45% of the whole gene. This figure
decreases considerably in the bovine and caprine/ovine counterpart because of presence/absence of RTs observed in these species. In particular, the bovine CSN1S2 does not show the element B and C and, at the same time, includes expanded Bov-A monomers (Bov-A3 in cattle and Bov-A2, G in buffalo). Sheep and goat
have a similar gene structure and, when compared to buffalo, the
absence of elements C, G, I and N was noted together with the
presence of an extra Bov-tA3 and of Bov-A4 in the promoter region
and the intron 1, respectively. Ruminant retroposons are highly
informative genetic markers that can be considered a powerful
phylogenetic tool for clustering studies, animal evolutionary history, population structure and demography, rather than the setup
of methods for species discrimination in meat and dairy
product
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Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65363/1/j.1530-0277.1996.tb01149.x.pd