Quali sono gli aspetti prioritari nella professione infermieristica? Percezione degli infermieri delle sue priorità, di quelle aziendali e di quelle del paziente

Un indicatore di benessere dell’infermiere, può essere costituito dalla sua percezione di condivisione con l’organizzazione in cui lavora e degli utenti alla cui assistenza è deputato, del valore attribuito ai vari aspetti della sua professione e dei bisogni ad essa associati. In un’indagine empirica pilota sono state indagate la percezione di condivisione fra infermiere, organizzazione ed utenti del valore attribuito ai vari aspetti della professione. I soggetti sono 66 infermieri operanti in un grande ospedale romano, reclutati su base volontaria. Gli interrogativi proposti riguardavano i tre aspetti considerati dagli intervistati come prioritari nel loro lavoro, quelli che secondo la loro esperienza, fossero considerati prioritari dalla loro direzione organizzativa, e quelli ritenuti da loro prioritari, per l’utente. Successivamente è stato proposto un questionario di Martorello “La mia soddisfazione professionale e le proposte dell’azienda” riguardo alle seguenti aree: tecnologia, incentivazione economica, flessibilità e formazione. È stata riscontrata una discordanza significativa, nella percezione degli infermieri interpellati, tra le priorità lavorative per gli infermieri, relative soprattutto alla competenza professionale, quelle ritenute prioritarie per l’organizzazione, relative alla mera presenza in servizio e quelle ritenute prioritarie per i pazienti, relative soprattutto alla relazione d’aiuto

A novel duplex ACRS-PCR for composite CSN1S1-CSN3 genotype discrimination in domestic buffalo

This short communication aimed to develop a fast and straightforward method for the simultaneous discrimination in buffaloes of the alleles CSN1S1 A/B and CSN3 X1/X2 using a single protocol. DNA was isolated from 219 individual blood samples. A duplex artificially created restriction site (ACRS-PCR) was accomplished using two pairs of primers generating 86 bp (CSN1S1) and 160 bp (CSN3). Amplicons were contemporary digested by MboI and HinfI for the identification of genotypes. The double simultaneous amplification and digestion proved to be effective for allele identification. The method resulted particularly quick due to the small PCR amplicons and fast digest enzymes that allowed both a rapid amplification (about 1 h and 40 min) and digestion in 10 min. Population analysis indicated that minor allele frequencies were CSN1S1 A (0.425) and CSN3 X2 (0.306). Linkage disequilibrium (LD) showed r2¼0.46, and haplotype analysis revealed all four possible combinations with higher frequency for the CSN1S1 B-CSN3 X1 (0.553). Considering the tremendous economic impact of the CSN1S1–CSN3 variants on the dairy production in buffalo, this method, applicable immediately after the birth from any DNA source, may speed up the selection of sires and dams’ lines with more favourable genotype

Artidactyla retroposons detection at the Mediterranean river buffalo CSN1S2 gene

The αs2-casein is an important phosphoprotein secreted in ruminants’ milk but, nowadays, the encoding gene (CSN1S2) has not been characterized in buffaloes. In this study, we report the first characterization of buffalo CSN1S2 gene and its artiodactyla retroposons (RTs) distribution. For this purpose, we amplified and sequenced the whole gene of 2 Mediterranean river subjects eared in Campania (Italy). The gene includes 1025 bp of coding (18 exons) and 17578bp of intronic regions, 937 bp at the 5’-end and 707 bp at the 3’-end and it is characterized by 13 RTs. The first (named A) is located in the promoter region and appears to be a Bov-tA2. Further 2 elements are located in intron 1 (B and C) and show a strong similarity with L1_Art sequence. Two Bov-tA2 are located in the intron 2 (D and E). The intron eight includes a Bov-B (F), whereas the intron 12 contains a Bov-A2 (G). Five additional retroposons (H, I, L, M, N) are located in the intron 13 (Bov-tA1), 15 (Bov-A2) and 17 (Bov-tA1, Bov-B, Bov-A2). Finally, a Bov-tA2 (O) is located in the 3’ UT, near to the last exon. The observed RTs represent 19.45% of the whole gene. This figure decreases considerably in the bovine and caprine/ovine counterpart because of presence/absence of RTs observed in these species. In particular, the bovine CSN1S2 does not show the element B and C and, at the same time, includes expanded Bov-A monomers (Bov-A3 in cattle and Bov-A2, G in buffalo). Sheep and goat have a similar gene structure and, when compared to buffalo, the absence of elements C, G, I and N was noted together with the presence of an extra Bov-tA3 and of Bov-A4 in the promoter region and the intron 1, respectively. Ruminant retroposons are highly informative genetic markers that can be considered a powerful phylogenetic tool for clustering studies, animal evolutionary history, population structure and demography, rather than the setup of methods for species discrimination in meat and dairy product

Effects of Disulfiram on Positron Emission Tomography and Neuropsychological Studies in Severe Chronic Alcoholism

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65363/1/j.1530-0277.1996.tb01149.x.pd