Evaluation of chitosan-GP hydrogel biocompatibility in osteochondral defects: an experimental approach

Background: Articular cartilage, because of its avascular nature, has little capacity for spontaneous healing, and tissue engineering approaches, employing different biomaterials and cells, are under development. Among the investigated biomaterials are the chitosan-based hydrogels. Although thoroughly studied in other mammalian species, studies are scarce in equines. So, the aim of the present study was to investigate the biocompatibility of chitosan-GP in horse joints submitted to high mechanical loads.Results: An osteochondral defect was created by arthroscopy in the medial surface of lateral trochlea of talus of left or right leg, randomly selected, from six healthy geldings. the defect was filled up with chitosan-GP. the contralateral joint received an identical defect with no implant. the chondral fragment removed to produce the defect was collected, processed and used as the Initial sample (normal cartilage) for histology, immunohistochemistry, and metabolic labelling of PGs. After 180 days, the repair tissues were collected, and also analyzed. At the end of the experiment (180 days after lesion), the total number of cells per field in repair tissues was equal to control, and macrophages and polymorphonuclear cells were not detected, suggesting that no significant inflammation was present. These cells were able to synthesize type II collagen and proteoglycans (PGs). Nevertheless, the cell population in these tissues, both in presence of chitosan-GP and in untreated controls, were heterogeneous, with a lower proportion of type II collagen-positives cells and some with a fibroblastic aspect. Moreover, the PGs synthesized in repair tissues formed in presence or absence of chitosan-GP were similar to those of normal cartilage. However, the chitosan-GP treated tissue had an disorganized appearance, and blood vessels were present.Conclusions: Implanted chitosan-GP did not evoke an important inflammatory reaction, and permitted cell growth. These cells were able to synthesize type II collagen and PGs similar to those synthesized in normal cartilage and in healing tissue without implant, indicating its chondrocyte nature.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ São Paulo, Fac Med Vet & Zootecnia, Dept Cirurgia, BR-09500900 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, BrazilUniv São Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, BR-09500900 São Paulo, BrazilUniv São Paulo, Fac Med Vet & Zootecnia, Dept Patol, BR-09500900 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, BrazilWeb of Scienc

Limits to gauge coupling in the dark sector set by the non-observation of instanton-induced decay of Super-Heavy Dark Matter in the Pierre Auger Observatory data

We investigate instanton-induced decay processes of super-heavy dark matter particles $X$ produced during the inflationary epoch. Using data collected at the Pierre Auger Observatory we derive a bound on the reduced coupling constant of gauge interactions in the dark sector: $\alpha_X^{\rm eff} \lesssim 0.09$, for $10^{10} < M_X/{\rm GeV} < 10^{16}$. We show that this upper limit on $\alpha_X^{\rm eff}$ is complementary to that obtained from the non-observation of tensor modes in the cosmic microwave background

Limits to gauge coupling in the dark sector set by the non-observation of instanton-induced decay of Super-Heavy Dark Matter in the Pierre Auger Observatory data

We investigate instanton-induced decay processes of super-heavy dark matter particles $X$ produced during the inflationary epoch. Using data collected at the Pierre Auger Observatory we derive a bound on the reduced coupling constant of gauge interactions in the dark sector: $\alpha_X^{\rm eff} \lesssim 0.09$, for $10^{10} < M_X/{\rm GeV} < 10^{16}$. We show that this upper limit on $\alpha_X^{\rm eff}$ is complementary to that obtained from the non-observation of tensor modes in the cosmic microwave background

Limits to gauge coupling in the dark sector set by the non-observation of instanton-induced decay of Super-Heavy Dark Matter in the Pierre Auger Observatory data

We investigate instanton-induced decay processes of super-heavy dark matter particles $X$ produced during the inflationary epoch. Using data collected at the Pierre Auger Observatory we derive a bound on the reduced coupling constant of gauge interactions in the dark sector: $\alpha_X^{\rm eff} \lesssim 0.09$, for $10^{10} < M_X/{\rm GeV} < 10^{16}$. We show that this upper limit on $\alpha_X^{\rm eff}$ is complementary to that obtained from the non-observation of tensor modes in the cosmic microwave background

Limits to gauge coupling in the dark sector set by the non-observation of instanton-induced decay of Super-Heavy Dark Matter in the Pierre Auger Observatory data

We investigate instanton-induced decay processes of super-heavy dark matter particles $X$ produced during the inflationary epoch. Using data collected at the Pierre Auger Observatory we derive a bound on the reduced coupling constant of gauge interactions in the dark sector: $\alpha_X^{\rm eff} \lesssim 0.09$, for $10^{10} < M_X/{\rm GeV} < 10^{16}$. We show that this upper limit on $\alpha_X^{\rm eff}$ is complementary to that obtained from the non-observation of tensor modes in the cosmic microwave background