Enzymatic surface hydrolysis of PET enhances bonding in PVC coating

The effect of polyesterase preparations from Thermomyces lanuginosus and Beauveria brongniartii on the hydrophilicity of PET materials was assessed. As a result of polyesterase treatment the hydrophilicity of PET fabrics was increased by up to 8 cm in terms of rising height with increases in surface tension from 6.2 mNm (heat-inactivated control samples) to above 8 mNm. Both enzymes were able to increase the amount of hydroxyl groups on PET from 90 to a maximum of 182 mmol kg-1, while only the B. brongniartii polyesterase released significant amounts of terephthalic acid from PET. Enzymatic surface hydrolysis of PET increased the bonding strength in PVC coating to 13.40 daN 5cm-1 using 0.5% adhesive compared to 11.5 daN 5cm-1 obtained without enzyme pretreatment and 6% of adhesive.The research was financed by the SFG, the FFG, the city of Graz and the province of Styria and by European Commission within the project GRD 2000-30110 Biosynte

Life cycle of Harmonia axyridis in central Europe

The development and reproduction, and maximum number of generations across seasons were ascertained for populations of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) in Central Europe. Number of generations was calculated using the thermal characteristics of development and summation of daily average air temperatures, in 2007–2016. This species can produce 2–3 generations per year. However, there is insufficient time for it to achieve its full reproductive potential. Delayed emigration from hibernacula (late April) shortened the period of seasonal activity by 1–6%. Breeding occurred from May to mid September. Oviposition continued into late summer, which left insufficient time for the late-hatching immatures to complete their development before the onset of winter. A high mobility, opportunistic use of prey patches and weak tendency to enter dormancy provide the potential for population increase by extending the period of development of immatures at the risk of failure if the cold period comes early

Ellipticine cytotoxicity to cancer cell lines — a comparative study

Ellipticine is a potent antineoplastic agent exhibiting multiple mechanisms of action. This anticancer agent should be considered a pro-drug, whose pharmacological efficiency and/or genotoxic side effects are dependent on its cytochrome P450 (CYP)- and/or peroxidase-mediated activation to species forming covalent DNA adducts. Ellipticine can also act as an inhibitor or inducer of biotransformation enzymes, thereby modulating its own metabolism leading to its genotoxic and pharmacological effects. Here, a comparison of the toxicity of ellipticine to human breast adenocarcinoma MCF-7 cells, leukemia HL-60 and CCRF-CEM cells, neuroblastoma IMR-32, UKF-NB-3 and UKF-NB-4 cells and U87MG glioblastoma cells and mechanisms of its action to these cells were evaluated. Treatment of all cells tested with ellipticine resulted in inhibition of cell growth and proliferation. This effect was associated with formation of two covalent ellipticine-derived DNA adducts, identical to those formed by 13-hydroxy- and 12-hydroxyellipticine, the ellipticine metabolites generated by CYP and peroxidase enzymes, in MCF-7, HL-60, CCRF-CEM, UKF-NB-3, UKF-NB-4 and U87MG cells, but not in neuroblastoma UKF-NB-3 cells. Therefore, DNA adduct formation in most cancer cell lines tested in this comparative study might be the predominant cause of their sensitivity to ellipticine treatment, whereas other mechanisms of ellipticine action also contribute to its cytotoxicity to neuroblastoma UKF-NB-3 cells

Development of the European Ladybirds Smartphone Application: A Tool for Citizen Science

Wildlife observations submitted by volunteers through citizen science initiatives are increasingly used within research and policy. Ladybirds are popular and charismatic insects, with most species being relatively easy to identify from photographs. Therefore, they are considered an appropriate taxonomic group for engaging people through citizen science initiatives to contribute long-term and large-scale datasets for use in many different contexts. Building on the strengths of a mass participation citizen science survey on ladybirds in the United Kingdom, we have developed a mobile application for ladybird recording and identification across Europe. The main aims of the application are to: (1) compile distribution data for ladybird species throughout Europe, and use this to assess changes in distribution over time; (2) connect and engage people in nature and increase awareness about the diversity and ecological importance of ladybirds. In developing the application we first constructed a database including ladybird species from the United Kingdom, Czech Republic, Slovakia, Italy, Belgium, and Portugal with associated information on relevant morphological features (e.g., size, main color, pronotum pattern) to inform identification. Additionally, the species were assessed on the basis of probability of occurrence within each country which enables users to reduce the number of species to only those with relevance to the location of the recorder. This is amongst the first collaborative citizen science approaches aimed at involving participants across Europe in recording a group of insects. In the near future, we aim to expand the use of the application to all countries in Europe

The organisation of physiotherapy for people with multiple sclerosis across Europe: a multicentre questionnaire survey

Background Understanding the organisational set-up of physiotherapy services across different countries is increasingly important as clinicians around the world use evidence to improve their practice. This also has to be taken into consideration when multi-centre international clinical trials are conducted. This survey aimed to systematically describe organisational aspects of physiotherapy services for people with multiple sclerosis (MS) across Europe. Methods Representatives from 72 rehabilitation facilities within 23 European countries completed an online web-based questionnaire survey between 2013 and 2014. Countries were categorised according to four European regions (defined by United Nations Statistics). Similarities and differences between regions were examined. Results Most participating centres specialized in rehabilitation (82 %) and neurology (60 %), with only 38 % specialising in MS. Of these, the Western based Specialist MS centres were predominately based on outpatient services (median MS inpatient ratio 0.14), whilst the Eastern based European services were mostly inpatient in nature (median MS inpatient ratio 0.5). In almost all participating countries, medical doctors - specialists in neurology (60 %) and in rehabilitation (64 %) - were responsible for referral to/prescription of physiotherapy. The most frequent reason for referral to/prescription of physiotherapy was the worsening of symptoms (78 % of centres). Physiotherapists were the most common members of the rehabilitation team; comprising 49 % of the team in Eastern countries compared to approximately 30 % in the rest of Europe. Teamwork was commonly adopted; 86 % of centres based in Western countries utilised the interdisciplinary model, whilst the multidisciplinary model was utilised in Eastern based countries (p = 0.046). Conclusion This survey is the first to provide data about organisational aspects of physiotherapy for people with MS across Europe. Overall, care in key organisational aspects of service provision is broadly similar across regions, although some variations, for example the models of teamwork utilised, are apparent. Organisational framework specifics should be considered anytime a multi-centre study is conducted and results from such studies are applied.PubMedWoSScopu

Cancer Cell Invasion Is Enhanced by Applied Mechanical Stimulation

Metastatic cells migrate from the site of the primary tumor, through the stroma, into the blood and lymphatic vessels, finally colonizing various other tissues to form secondary tumors. Numerous studies have been done to identify the stimuli that drive the metastatic cascade. This has led to the identification of multiple biochemical signals that promote metastasis. However, information on the role of mechanical factors in cancer metastasis has been limited to the affect of compliance. Interestingly, the tumor microenvironment is rich in many cell types including highly contractile cells that are responsible for extensive remodeling and production of the dense extracellular matrix surrounding the cancerous tissue. We hypothesize that the mechanical forces produced by remodeling activities of cells in the tumor microenvironment contribute to the invasion efficiency of metastatic cells. We have discovered a significant difference in the extent of invasion in mechanically stimulated verses non-stimulated cell culture environments. Furthermore, this mechanically enhanced invasion is dependent upon substrate protein composition, and influenced by topography. Finally, we have found that the protein cofilin is needed to sense the mechanical stimuli that enhances invasion. We conclude that other types of mechanical signals in the tumor microenvironment, besides the rigidity, can enhance the invasive abilities of cancer cells in vitro. We further propose that in vivo, non-cancerous cells located within the tumor micro-environment may be capable of providing the necessary mechanical stimulus during the remodeling of the extracellular matrix surrounding the tumor