Environmental Effect on New Released Genotypes of Graminaceous and Leguminous Forage Perennial Crops

Genotypes of perennial leguminous (alfalfa) and graminaceous (cocksfoot and tall fescue) were evaluated for adaptability in a typical Mediterranean environment (Foggia, southern Italy). The species were field evaluated to detect biomass yield potential under different types of watering. Irrigations were applied considering rainfall and FAO cultural growth coefficient for reintegrating the 75% of the evaporated water from Class A water pan. The forage harvest was applied when the shoots of the varieties reached the 10 and 50% of flowering and heading stage for leguminous and graminaceous, respectively. A split-plot experimental design, with irrigation in the main plot, was adopted. The parameters considered were dry matter, stand density, leaf-stem ratio. The preliminary results evidenced a significant difference among treatments. The species and the varieties show a different adaptability to the weather conditions. Alfalfa had higher biomass production than graminaceous while irrigation appears as the main agronomical factor allowings increase of forage yield in the Mediterranean areas

Quantitative and Qualitative Evaluation of Legume Forage Species in Southern Italy

Legume herbage quality and quantity depend on genetic background to be able to sustain metabolic activity under increasingly harsh environmental conditions. Field experiments were carried out in Foggia, a southern Italian town (a typical Mediterranean location) on varieties and ecotypes of two perennial legume (sainfoin and sulla) mostly widespread in Mediterranean environments. The experiment compared irrigated and non-irrigated conditions and two different seed rates. The aim of the study was to evaluate the effect of agronomic factors on herbage production and the nutritive value of the forage. Irrigation and higher seed rate treatment increased dry matter yield in both species. The genotypes of both species differed for agronomic adaptation and in nutritive characteristics which were influenced by irrigation treatment. Crude protein (CP) contents under irrigated treatment was lower than non irrigated only in sulla. Percentages of neutral detergent fibre (NDF) and acid detergent fiber (ADF) were influenced by lower seed rate. The genotypes exploit their genetic potentiality mainly under irrigated condition in sainfoin and sulla. Lower seed rate was more suitable under non irrigated conditions. The nutritive value, in both species, was related to the dry matter production and leaf stem proportion of genotypes

Water Relations in Berseem Cultivars Under Drought Stress

Berseem clover (Trifolium alexandrinum L.) is a crop of particular interest in semi-arid regions, used for herbage and hay productions. However, its seed yield is reduced by drought stress that occurs during seed filling. In this study five cultivars (Axi, Big Bee, Lilibeo, Sacromonte and Saniros) subjected to four irrigation frequencies in controlled environment, were compared for their physiological responses to subsequent water stress period (3 days). Some parameters (leaf water potential (), osmotic potential (þ), relative water content (RWC) and gravimetric soil water content (GSWC)) related to plant water status were recorded. Plants subjected to previous periods of water stress were less sensitive to final drought stress than plants never stressed (control). Among cultivars examined, Lilibeo showed significantly higher values of , þ and RWC. Therefore, it seems to be the most tolerant cultivar for its ability to mantain high water status under water stress conditions

Combined flow-based imaging assessment of optimal cardiac resynchronization therapy pacing vector: A case report

Background: There are still many pendent issues about the effective evaluation of cardiac resynchronization therapy impact on functional mitral regurgitation. In order to reduce the intrinsic difficulties of quantification of functional mitral regurgitation itself, an automatic quantification of real-time three-dimensional full-volume color Doppler transthoracic echocardiography was proposed as a new, rapid, and accurate method for the assessment of functional mitral regurgitation severity. Recent studies suggested that images of left ventricle flow by echo-particle imaging velocimetry could be a useful marker of synchrony. Echo-particle imaging velocimetry has shown that regional anomalies of synchrony/synergy of the left ventricle are related to the alteration, reduction, or suppression of the physiological intracavitary pressure gradients. Case summary: We describe a case in which the two technologies are used in combination during acute echocardiographic optimization of left pacing vector in a 63-year-old man, Caucasian, who showed worsening heart failure symptoms a few days after an implant, and the effect of the device\u2019s optimization at 6-month follow-up. Discussion: The degree of realignment of hemodynamic forces, with quantitative analysis of the orientation of blood flow momentum (\u3c6), can represent improvement of fluid dynamics synchrony of the left ventricle, and explain, with a new deterministic parameter, the effects of cardiac resynchronization therapy on functional mitral regurgitation. Realtime three-dimensional color flow Doppler quantification is feasible and accurate for measurement of mitral inflow, left ventricular outflow stroke volumes, and functional mitral regurgitation severity. Conclusion: This clinical case offers an innovative and accurate approach for acute echocardiographic optimization of left pacing vector. It shows clinical utility of combined three-dimensional full-volume color Doppler transthoracic echocardiography/echo-particle imaging velocimetry assessment to increase response to cardiac resynchronization therapy, in terms of reduction of functional mitral regurgitation, improving fluid dynamics synchrony of the left ventricle

Governing multicultural Brussels: paradoxes of a multi-level, multi-cultural, multi-national urban anomaly

Updating our earlier work on Brussels as the paradigm of a multi-level, multi-cultural, multi-national city, and in the context of Brussels’s recent troubled emergence as the epicentre of violent conflict between radical political Islam and the West, this paper sets out the paradoxical intersection of national (i.e. Flemish and Francophone), non-national and ethnic minority politics in a city placed as a multi-cultural and multi-national ‘urban anomaly’ at the heart of linguistic struggle of the two dominant Belgian communities. Brussels is one of the three Regions of the Belgian federal model alongside Flanders and Wallonia. It is also an extraordinarily diverse and cosmopolitan city, in which a mixed language Belgian population lives alongside very high numbers of resident non-nationals, including European elites, other European immigrant workers, and immigrants from Africa and Asia. After laying out the complex distribution of power and competences within the Belgian federal structure, we explore whether these structures have worked over the years to include or exclude disadvantaged ethnic groups. To better understand these processes, we introduce our view of the multi-level governance perspective

Effect of expression of adenine phosphoribosyltransferase on the in vivo anti-tumor activity of prodrugs activated by E. coli purine nucleoside phosphorylase

The use of E. coli purine nucleoside phosphorylase (PNP) to activate prodrugs has demonstrated excellent activity in the treatment of various human tumor xenografts in mice. E. coli PNP cleaves purine nucleoside analogs to generate toxic adenine analogs, which are activated by adenine phosphoribosyl transferase (APRT) to metabolites that inhibit RNA and protein synthesis. We created tumor cell lines that encode both E. coli PNP and excess levels of human APRT, and have used these new cell models to test the hypothesis that treatment of otherwise refractory human tumors could be enhanced by overexpression of APRT. In vivo studies with 6-methylpurine-2′-deoxyriboside (MeP-dR), 2-F-2′-deoxyadenosine (F-dAdo) or 9-β-D-arabinofuranosyl-2-fluoroadenine 5′-monophosphate (F-araAMP) indicated that increased APRT in human tumor cells coexpressing E. coli PNP did not enhance either the activation or the anti-tumor activity of any of the three prodrugs. Interestingly, expression of excess APRT in bystander cells improved the activity of MeP-dR, but diminished the activity of F-araAMP. In vitro studies indicated that increasing the expression of APRT in the cells did not significantly increase the activation of MeP. These results provide insight into the mechanism of bystander killing of the E. coli PNP strategy, and suggest ways to enhance the approach that are independent of APRT

Prodrug converting enzyme gene delivery by L. monocytogenes

<p>Abstract</p> <p>Background</p> <p><it>Listeria monocytogenes </it>is a highly versatile bacterial carrier system for introducing protein, DNA and RNA into mammalian cells. The delivery of tumor antigens with the help of this carrier into tumor-bearing animals has been successfully carried out previously and it was recently reported that <it>L. monocytogenes </it>is able to colonize and replicate within solid tumors after local or even systemic injection.</p> <p>Methods</p> <p>Here we report on the delivery of two prodrug converting enzymes, purine-deoxynucleoside phosphorylase (PNP) and a fusion protein consisting of yeast cytosine deaminase and uracil phosphoribosyl transferase (FCU1) into cancer cells in culture by <it>L. monocytogenes</it>. Transfer of the prodrug converting enzymes was achieved by bacterium mediated transfer of eukaryotic expression plasmids or by secretion of the proteins directly into the host cell cytosol by the infecting bacteria.</p> <p>Results</p> <p>The results indicate that conversion of appropriate prodrugs to toxic drugs in the cancer cells occured after both procedures although <it>L. monocytogenes</it>-mediated bactofection proved to be more efficient than enzyme secretion 4T1, B16 and COS-1 tumor cells. Exchanging the constitutively P_CMV-promoter with the melanoma specific P_4xTETP-promoter resulted in melanoma cell-specific expression of the prodrug converting enzymes but reduced the efficiencies.</p> <p>Conclusion</p> <p>These experiments open the way for bacterium mediated tumor specific activation of prodrugs in live animals with tumors.</p