Difficulties in the energy renovation processes of district heating buildings. Two case studies in a temperate climate

Renovation at district scale is a key strategy to reduce CO2 emissions and energy consumptions by optimising the implementation of renewable energy sources and taking advantage of economies of scale. In this context, this paper focuses on assessing the positive impacts and difficulties after the energy rehabilitation of thermal envelopes in two buildings that belong to two different District Heating systems. The methodology is based on the comparative analysis of indoor temperatures data and energy consumption data of 17 monitored dwellings. The results showed a significant association between the improvement of envelopes and the increase of indoor temperatures in winter (β=0,644). Due to some technical and social barriers, the heating system was not regulated after the rehabilitation, so energy consumption was unnecessarily high, there were situations of indoor overheating in winter (maximum average indoor temperatures between 24-26°C) and these issues produced dissatisfaction on neighbours. In order to avoid these negative consequences, some recommendations are provided, such as informing neighbours about expectations in each step of the long rehabilitation process, reconsidering payments to promote the envelope rehabilitation but maintaining a fixed cost to protect vulnerable groups, and promoting post-occupational studies that contribute to the viability and up-date of this kind of District Heating systems

HAMLET forms annular oligomers when deposited with phoshpolipid monolayers

Recently, the anticancer activity of human α-lactalbumin made lethal to tumor cells (HAMLET) has been linked to its increased membrane affinity in vitro, at neutral pH, and ability to cause leakage relative to the inactive native bovine α-lactalbumin (BLA) protein. In this study, atomic force microscopy resolved membrane distortions and annular oligomers (AOs) produced by HAMLET when deposited at neutral pH on mica together with a negatively charged lipid monolayer. BLA, BAMLET (HAMLET's bovine counterpart) and membrane-binding Peptide C, corresponding to BLA residues 75–100, also form AO-like structures under these conditions but at higher subphase concentrations than HAMLET. The N-terminal Peptide A, which binds to membranes at acidic but not at neutral pH, did not form AOs. This suggests a correlation between the capacity of the proteins/peptides to integrate into the membrane at neutralpH—as observed by liposome content leakage and circular dichroism experiments—and the formation of AOs, albeit at higher concentrations. Formation of AOs, which might be important to HAMLET's tumor toxic action, appears related to the increased tendency of the protein to populate intermediately folded states compared to the native protein, the formation of which is promoted by, but not uniquely dependent on, the oleic acid molecules associated with HAMLET.publishedVersio

Light-Responsive Self-Assembled Materials by Supramolecular Post-Functionalization via Hydrogen Bonding of Amphiphilic Block Copolymers

This work was supported by the MINECO, Spain, under Projects MAT2014-59187-R and MAT2014-55205-P, FEDER funding, and Aragon Government. A.C. acknowledges MINE- ́ CO for his Ph.D. grant. A.M.-F. expresses thanks for the financial support of the Generalitat Valenciana for his APOSTD/2013/054 grant. The authors would like to acknowledge the Servicio General de Apoyo a la Investigacion (SAI) ́ and the Advanced Microscopy Laboratory (LMA) of the Universidad de Zaragoza and the Servei de Microscopia of the ̀ Universitat Autonoma de Barcelona for the TEM and cryo- ̀TEM observations. The authors additionally acknowledge the use of the CEQMA Services of the Universidad de ZaragozaCSIC. The authors also thank the IACS (Aragon Health ́ Sciences Institute) for the confocal microscope studiesPeer reviewedPostprintPostprin

Encouraging natural ventilation to improve indoor environmental conditions at schools. Case studies in the north of Spain before and during COVID

The COVID pandemic has strongly affected daily life both in Spanish schools and worldwide. Providing the best environmental conditions for children allowing face-to-face learning with healthy and safe indoor spaces is a challenge. In the present study, empirical research about how these environmental conditions change with COVID is presented comparing the situation from March 2020 to January 2021. The methodology combines surveys conducted in nine schools with a case study in a selected school where a detailed monitoring of the building was developed during both heating seasons. This data ana- lyzes the impact of the new COVID prevention protocols on indoor environmental conditions (especially those related to natural ventilation). Results show a mean CO2 reduction of 1,400 ppm, having in the sec- ond term values around 1,000 ppm, although temperatures diminished nearly 2 °C to mean values of 18 °C. Evolution of temperature and CO2 concentration throughout the day was also analyzed, being these indoor conditions especially important for the children with poorer health. Mechanical ventilation with heating recovery should complement natural ventilation, at least during the coldest months or hours of the day, although systems have to be carefully designed and installed to work effectively

Fucoxanthin’s Optimization from Undaria pinnatifida Using Conventional Heat Extraction, Bioactivity Assays and In Silico Studies

Brown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.Authors are grateful to the Bio Based Industries Joint Undertaking (JU) under grant agreement no. 888003 UP4HEALTH Project (H2020-BBI-JTI-2019). The JU receives support from the European Union’s Horizon 2020 research and innovation program and the Bio Based Industries Consortium. The project SYSTEMIC Knowledge Hub on Nutrition and Food Security, has received funding from national research funding parties in Belgium (FWO), France (INRA), Germany (BLE), Italy (MIPAAF), Latvia (IZM), Norway (RCN), Portugal (FCT), and Spain (AEI) as a result of a joint action by JPI HDHL, JPI-OCEANS, and FACCE-JPI that was launched in 2019 under the ERA-NET ERA-HDHL (n_ 696295).info:eu-repo/semantics/publishedVersio

DNA hypermethylation of the serotonin receptor type-2A gene is associated with a worse response to a weight loss intervention in subjects with metabolic syndrome

Understanding the regulation of gene activities depending on DNA methylation has been the subject of much recent study. However, although polymorphisms of the HTR2A gene have been associated with both obesity and psychiatric disorders, the role of HTR2A gene methylation in these illnesses remains uncertain. The aim of this study was to evaluate the association of HTR2A gene promoter methylation levels in white blood cells (WBC) with obesity traits and depressive symptoms in individuals with metabolic syndrome (MetS) enrolled in a behavioural weight loss programme. Analyses were based on 41 volunteers (mean age 49 ± 1 year) recruited within the RESMENA study. Depressive symptoms (as determined using the Beck Depression Inventory), anthropometric and biochemical measurements were analysed at the beginning and after six months of weight loss treatment. At baseline, DNA from WBC was isolated and cytosine methylation in the HTR2A gene promoter was quantified by a microarray approach. In the whole-study sample, a positive association of HTR2A gene methylation with waist circumference and insulin levels was detected at baseline. Obesity measures significantly improved after six months of dietary treatment, where a lower mean HTR2A gene methylation at baseline was associated with major reductions in body weight, BMI and fat mass after the treatment. Moreover, mean HTR2A gene methylation at baseline significantly predicted the decrease in depressive symptoms after the weight loss treatment. In conclusion, this study provides newer evidence that hypermethylation of the HTR2A gene in WBC at baseline is significantly associated with a worse response to a weight-loss intervention and with a lower decrease in depressive symptoms after the dietary treatment in subjects with MetS

Una ciudad sostenible para una familia sostenible

El objetivo de este trabajo se resume en su título, suficientemente expresivo en sí mismo, como guía del planteamiento de una contribución arquitectónica que responda al interés suscitado por el reto que el siglo XXI, con sus transformaciones, plantea abiertamente hoy a la familia incluso a su propia permanencia futura, es decir, a su sostenibilidad; un reto que adquiere también notas características si se tiene en cuenta que la arquitectura actual además de necesitar una mayor conciencia de servir directamente a la familia está dando clara prioridad a cuestiones relacionadas con la sostenibilidad global. Con ello apunta una pauta para su actuación futura, como sucede también en otros muchos campos filosóficos, científicos o técnicos. Lo que nos proponemos aquí es esbozar los rasgos fundamentales que deben inspirar proyectos más específicos, dirigidos a promover la sostenibilidad de la familia desde la Arquitectura, y, en general, un desarrollo sostenible desde la familia. Entendemos, en efecto, que la familia es esencial para la sostenibilidad del sistema global de desarrollo, en todos sus aspectos, incluido el urbano. La noción de desarrollo sostenible surge de la alarma ecológica, al hacerse más evidentes las consecuencias negativas que provoca un desarrollo incontrolado, ligado a cierto estilo de vida, de economía y de mercado, etc. Mantener el desarrollo y sus ventajas de modo que puedan disfrutarlo los que nos sucedan, es hacerlo sostenible. Del mismo modo, mantener la familia y sus valores en las futuras generaciones, asumiendo nuevos retos y resolviendo las influencias negativas que están surgiendo en nuestros días, sería, en consonancia con lo dicho, hacerla sostenible. Si en su primer planteamiento tanto la ecología como la sostenibilidad se consideraron a nivel medioambiental, ambos términos se han ido progresivamente ampliando hasta llegar a tener en cuenta todos los aspectos del equilibrio mundial. En esta comunicación, al incluir a la familia como sujeto activo y pasivo dentro de la ciudad, se plantea una específica ampliación de estas nociones. En este contexto, tiene interés mencionar la reciente aportación del Papa Francisco a la cuestión del desarrollo, concretada en la publicación de la Encíclica Laudato Si’1. En este documento, en efecto, Francisco hace llegar al gran público un concepto amplio de desarrollo, que expande las ideas de ecología y sostenibilidad desde lo material a lo cultural, lo vital y lo moral, incorporando incluso la dimensión espiritual de la persona. Dentro de este marco, que resalta las dimensiones éticas y no solamente técnicas del desarrollo, se incluyen referencias a la familia, a la arquitectura y a la ciudad, parcialmente incluidas en el tratamiento que se ha venido dando a la cuestión desde hace algunas décadas

Brain catecholamine depletion and motor impairment in a Th knock-in mouse with type B tyrosine hydroxylase deficiency

Tyrosine hydroxylase catalyses the hydroxylation of L-tyrosine to l-DOPA, the rate- limiting step in the synthesis of catecholamines. Mutations in the TH gene encoding tyrosine hydroxylase are associated with the autosomal recessive disorder tyrosine hydroxylase deficiency, which manifests phenotypes varying from infantile parkinsonism and DOPA-responsive dystonia, also termed type A, to complex encephalopathy with perinatal onset, termed type B. We generated homozygous Th knock-in mice with the mutation Th-p.R203H, equivalent to the most recurrent human mutation associated with type B tyrosine hydroxylase deficiency (TH-p.R233H), often unresponsive to l-DOPA treatment. The Th knock-in mice showed normal survival and food intake, but hypotension, hypokinesia, reduced motor coordination, wide-based gate and catalepsy. This phenotype was associated with a gradual loss of central catecholamines and the serious manifestations of motor impairment presented diurnal fluctuation but did not improve with standard l-DOPA treatment. The mutant tyrosine hydroxylase enzyme was unstable and exhibited deficient stabilization by catecholamines, leading to decline of brain tyrosine hydroxylase-immunoreactivity in the Th knock-in mice. In fact the substantia nigra presented an almost normal level of mutant tyrosine hydroxylase protein but distinct absence of the enzyme was observed in the striatum, indicating a mutation-associated mislocalization of tyrosine hydroxylase in the nigrostriatal pathway. This hypomorphic mouse model thus provides understanding on pathomechanisms in type B tyrosine hydroxylase deficiency and a platform for the evaluation of novel therapeutics for movement disorders with loss of dopaminergic input to the striatum

Metabolomics identifies changes in fatty acid and amino acid profiles in serum of overweight older adults following a weight loss intervention

The application of metabolomics in nutritional research may be a useful tool to analyse and predict the response to a dietary intervention. The aim of this study was to examine metabolic changes in serum samples following exposure to an energy-restricted diet (-15% of daily energy requirements) over a period of 8weeks in overweight and obese older adults (n=22) using a gas chromatography/mass spectrometry (GC/MS) metabolomic approach. After 8weeks, there were significant reductions in weight (7%) and metabolic improvement (glucose and lipid profiles). Metabolomic analysis found that total saturated fatty acids (SFAs), including palmitic acid (C16:0) and stearic acid (C18:0) and monounsaturated fatty acids (MUFAs), were significantly decreased after the 8-week intervention. Furthermore, palmitoleic acid (C16:1) was found to be a negative predictor of change in body fat loss. Both the total omega-6 and omega-3 polyunsaturated fatty acids (PUFAs) significantly decreased, although the overall total amounts of PUFAs did not. The branched chain amino acid (BCAA) isoleucine significantly decreased in the serum samples after the intervention. In conclusion, this study demonstrated that the weight loss intervention based on a hypocaloric diet identified changes in the metabolic profiles of serum in overweight and obese older adults, with a reduction in anthropometric and biochemical parameters also found

Air Trapping on Chest CT Is Associated with Worse Ventilation Distribution in Infants with Cystic Fibrosis Diagnosed following Newborn Screening

BACKGROUND: In school-aged children with cystic fibrosis (CF) structural lung damage assessed using chest CT is associated with abnormal ventilation distribution. The primary objective of this analysis was to determine the relationships between ventilation distribution outcomes and the presence and extent of structural damage as assessed by chest CT in infants and young children with CF. METHODS: Data of infants and young children with CF diagnosed following newborn screening consecutively reviewed between August 2005 and December 2009 were analysed. Ventilation distribution (lung clearance index and the first and second moment ratios [LCI, M(1)/M(0) and M(2)/M(0), respectively]), chest CT and airway pathology from bronchoalveolar lavage were determined at diagnosis and then annually. The chest CT scans were evaluated for the presence or absence of bronchiectasis and air trapping. RESULTS: Matched lung function, chest CT and pathology outcomes were available in 49 infants (31 male) with bronchiectasis and air trapping present in 13 (27%) and 24 (49%) infants, respectively. The presence of bronchiectasis or air trapping was associated with increased M(2)/M(0) but not LCI or M(1)/M(0). There was a weak, but statistically significant association between the extent of air trapping and all ventilation distribution outcomes. CONCLUSION: These findings suggest that in early CF lung disease there are weak associations between ventilation distribution and lung damage from chest CT. These finding are in contrast to those reported in older children. These findings suggest that assessments of LCI could not be used to replace a chest CT scan for the assessment of structural lung disease in the first two years of life. Further research in which both MBW and chest CT outcomes are obtained is required to assess the role of ventilation distribution in tracking the progression of lung damage in infants with CF