The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: Methodology

<p>Abstract</p> <p>Background</p> <p>Cryopreservation is the only widely applicable method of storing vital cells for nearly unlimited periods of time. Successful cryopreservation is essential for reproductive medicine, stem cell research, cord blood storage and related biomedical areas. The methods currently used to retrieve a specific cell or a group of individual cells with specific biological properties after cryopreservation are quite complicated and inefficient.</p> <p>Results</p> <p>The present study suggests a new approach in cryopreservation, utilizing the Individual Cell-based Cryo-Chip (i3C). The i3C is made of materials having appropriate durability for cryopreservation conditions. The core of this approach is an array of picowells, each picowell designed to maintain an individual cell during the severe conditions of the freezing - thawing cycle and accompanying treatments. More than 97% of cells were found to retain their position in the picowells throughout the entire freezing - thawing cycle and medium exchange. Thus the comparison between pre-freezing and post-thawing data can be achieved at an individual cell resolution. The intactness of cells undergoing slow freezing and thawing, while residing in the i3C, was found to be similar to that obtained with micro-vials. However, in a fast freezing protocol, the i3C was found to be far superior.</p> <p>Conclusions</p> <p>The results of the present study offer new opportunities for cryopreservation. Using the present methodology, the cryopreservation of individual identifiable cells, and their observation and retrieval, at an individual cell resolution become possible for the first time. This approach facilitates the correlation between cell characteristics before and after the freezing - thawing cycle. Thus, it is expected to significantly enhance current cryopreservation procedures for successful regenerative and reproductive medicine.</p

Mononeuritis multiplex following immune checkpoint inhibitors in malignant pleural mesothelioma

IntroductionMononeuritis multiplex is frequently related to vasculitic neuropathy and has been reported only sporadically as an adverse event of immune checkpoint inhibitors.MethodsCase series of three patients with mononeuritis multiplex—all with mesothelioma—identified in the databases of two French clinical networks (French Reference Center for Paraneoplastic Neurological Syndromes, Lyon; OncoNeuroTox, Paris; January 2015–October 2022) set up to collect and investigate n-irAEs on a nationwide level.ResultsThree patients (male; median age 86 years; range 72–88 years) had pleural mesothelioma and received 10, 4, and 6 cycles, respectively, of first-line nivolumab plus ipilimumab combined therapy. In patient 1, the neurological symptoms involved the median nerves, and in the other two patients, there was a more diffuse distribution; the symptoms were severe (common terminology criteria for adverse events, CTCAE grade 3) in all patients. Nerve conduction studies indicated mononeuritis multiplex in all patients. Peripheral nerve biopsy demonstrated necrotizing vasculitis in patients 1 and 3 and marked IgA deposition without inflammatory lesions in patient 2. Immune checkpoint inhibitors were permanently withdrawn, and corticosteroids were administered to all patients, leading to complete symptom regression (CTCAE grade 0, patient 2) or partial improvement (CTCAE grade 2, patients 1 and 3). During steroid tapering, patient 1 experienced symptom recurrence and spreading to other nerve territories (CTCAE grade 3); he improved 3 months after rituximab and cyclophosphamide administration.DiscussionWe report the occurrence of mononeuritis multiplex, a very rare adverse event of immune checkpoint inhibitors, in the three patients with mesothelioma. Clinicians must be aware of this severe, yet treatable adverse event

Reproduction naturelle de la race caucasique dans l'Amérique du Sud

De Moussy Martin. Reproduction naturelle de la race caucasique dans l'Amérique du Sud. In: Bulletins de la Société d'anthropologie de Paris, I° Série. Tome 5 fascicule 5, 1864. pp. 948-960

La religiosité est-elle un des caractères spéciaux du genre humain?

De Moussy Martin. La religiosité est-elle un des caractères spéciaux du genre humain?. In: Bulletins de la Société d'anthropologie de Paris, II° Série. Tome 1, 1866. pp. 105-121

Sur la reproduction des métis et l'atavisme

De Moussy Martin. Sur la reproduction des métis et l'atavisme. In: Bulletins de la Société d'anthropologie de Paris, I° Série. Tome 6, 1865. pp. 285-291

Carte des provinces de Salta et de Jujuy et d'une partie de la Bolivie / par le Dr. V. Martin de Moussy ; gravé par Kautz, Paris

1 mapa, col. Full de l'Atlas de Description géographique et statistique de la Confédération Argentine (Planche XVI). Paris: Firmin Didot Fres., 1860-64. 3. vol. Imp. Lemercier.47x61 c

Carte du Grand Chaco (Territoire Indien du Nord) et des contrées voisines pour servir a l'Histoire du Bassin de la Plata de 1520 à 1865 / par le Dr. V. Martin de Moussy ; gravé par Kautz ; Lit. Lemercier

1 Mapa, col. Full de l'Atlas de Description géographique et statistique de la Confédération Argentine (Planche XVIII). Paris: Firmin Didot Fres., 1860-64. 3. vol. Imp. Lemercier47x61 c

Carte de la province de Corrientes du Territoire des Missions et des pays adjacents

1 Mapa, col. - Full de l'Atlas de Description géographique et statistique de la Confédération Argentine (Planche VII). Paris: Imp. Lemercier47 x 61 c

Carte de la province de Mendoza de l'Araucanie et de la plus grande partie du Chili

1 Mapa, col. - Full de l'Atlas de Description géographique et statistique de la Confédération Argentine (Planche XIII). Paris: Firmin Didot Fres., 1860-64. 3. vol. Imp. Lemercier47 x 61 c

Carte des provinces de Salta et de Jujuy et d'une partie de la Bolivie / par le Dr. V. Martin de Moussy ; gravé par Kautz, Paris

1 mapa, col. Full de l'Atlas de Description géographique et statistique de la Confédération Argentine (Planche XVI). Paris: Firmin Didot Fres., 1860-64. 3. vol. Imp. Lemercier.47x61 c