Recycling of organic carbon near the sediment-water interface in coastal environments.

Detailed studies of benthic organic carbon recycling at a single non-bioturbated coastal site, including direct seasonal rate determinations and sediment-water dissolved carbon flux measurements, serve to test the usefulness of a simple kinetic model for degradation based strictly on sedimentary organic carbon distribution

Temporal variations in the stable carbon isotopic composition of methane emitted from Minnesota peatlands

The stable carbon isotopic composition of methane (δ13C) emitted from two peatland sites in the Marcell Experimental Forest in northern Minnesota was investigated during the snow‐free season of 1989–1990. A seasonal range in δ13C values of 13‰ was seen for a forested bog with heavier (13C enriched) methane emitted during the wanner summer months. This shift was correlated with water table level suggesting control by microbial oxidation. Methane from a nearby poor fen transitional to bog dominated by Carex oligosperma showed a similar temporal trend but with a much smaller range of 5‰ during the same time period and with no water table level correlation. The methane emitted from the fen was consistently heavier than that emitted by the bog

Carbon and hydrogen isotopic characterization of methane from wetlands and lakes of the Yukon-Kuskokwim Delta, western Alaska

The total methane flux to the troposphere from tundra environments of the Yukon-Kuskokwim Delta is dominated by emissions from wet meadow tundra (~75%) and small, organic-rich lakes (~20%). The mean δ13C value of methane diffusing into collar-mounted flux chambers from wet meadow environments near Bethel, Alaska, was -65.82±2.21‰ (±1 sigma, n=18) for the period July 10 to August 10, 1988. A minimum ebullition estimated for the 5% of total Delta area comprised of small lakes ranges from 0.34 to 9.7 × 1010g Ch4yr-1, which represents 0.6% to 17% of the total Delta methane emission. The δ13C and δD values of this ebullitive flux are -61.41±2.46‰ (n=38) and -341.8±18.2‰ (n=21), respectively. The methane in gas bubbles from two lakes is of modern, bomb carbon enriched, radiocarbon age. Gas bubble δ13C values varied from 2 to 5‰ seasonally, reaching heaviest values in midsummer; no such variations in δD values were observed

Field and laboratory studies of methane oxidation in an anoxic marine sediment: Evidence for a methanogen‐sulfate reducer consortium

Field and laboratory studies of anoxic sediments from Cape Lookout Bight, North Carolina, suggest that anaerobic methane oxidation is mediated by a consortium of methanogenic and sulfate‐reducing bacteria. A seasonal survey of methane oxidation and CO2 reduction rates indicates that methane production was confined to sulfate‐depleted sediments at all times of year, while methane oxidation occurred in two modes. in the summer, methane oxidation was confined to sulfate‐depleted sediments and occurred at rates lower than those of CO2 reduction. in the winter, net methane oxidation occurred in an interval at the base of the sulfate‐containing zone. Sediment incubation experiments suggest both methanogens and sulfate reducers were responsible for the observed methane oxidation. In one incubation experiment both modes of oxidation were partially inhibited by 2‐bromoethanesulfonic acid (a specific inhibitor of methanogens). This evidence, along with the apparent confinement of methane oxidation to sulfate‐depleted sediments in the summer, indicates that methanogenic bacteria are involved in methane oxidation. In a second incubation experiment, net methane oxidation was induced by adding sulfate to homogenized methanogenic sediments, suggesting that sulfate reducers also play a role in the process. We hypothesize that methanogens oxidize methane and produce hydrogen via a reversal of CO2 reduction. The hydrogen is efficiently removed and maintained at low concentrations by sulfate reducers. Pore water H2 concentrations in the sediment incubation experiments (while net methane oxidation was occurring) were low enough that methanogenic bacteria could derive sufficient energy for growth from the oxidation of methane. The methanogen‐sulfate reducer consortium is consistent not only with the results of this study, but may also be a feasible mechanism for previously documented anaerobic methane oxidation in both freshwater and marine environments

Consumption of dissolved organic carbon by Caribbean reef sponges

Sponges are conspicuous and abundant within the benthic fauna on Caribbean reefs. The ability of these organisms to efficiently capture carbon from particulate sources is well known and the importance of dissolved organic carbon (DOC) uptake has been recognized for several species. We surveyed DOC ingestion by seven sponge species common to Florida Keys reefs using nondisruptive sampling methods on undisturbed individuals. Three of the seven species exhibited significant DOC removal ranging from 13% to 24% of ambient concentrations. The tested species that removed DOC host large microbial consortia within their tissues, while the converse was observed for those that did not. This divergent behavior may suggest an important role for sponge associated microbes in the utilization of DOC by these species. The feeding behaviors of individuals of Xestospongia muta were then monitored over time to investigate its respiratory consumption of particulate and DOC. The uptake rates of dissolved oxygen (DO) and organic carbon by two undisturbed individuals revealed that DOC represented 96% of removed C, and that the tested individuals removed approximately equal quantities of C and DO. This demonstrates that X. muta largely satisfies its respiration demands through DOC consumption, and that DOC likely represents the dominant C source for biomass production and cell overturn in this species. These results further illustrate the metabolic importance of DOC to sponges, and suggest that these organisms are an important pathway for remineralizing organic matter on Caribbean reefs

Sponges represent a major source of inorganic nitrogen in Florida Bay (U.S.A.)

Florida Bay nutrient budgets have shown that the majority of existing and influent nitrogen (N) is in organic forms. Consequently, local remineralization processes have been found to regulate the supply of dissolved inorganic nitrogen (DIN). Sponges have dominated benthic animal biomass in Florida Bay and are known to influence local DIN concentrations through remineralization organic matter, yet the role of these organisms in local N budgets is largely unaddressed. We quantified the role of sponges in N cycling in Florida Bay during 2012–2013 by constructing an N budget for a sponge-rich basin. Surveys of sponge biomass conducted in Mystery Basin found sponges at 57 of the 59 assessed stations. Sponge population maxima reached 21 individuals m−2 and biomass contributions as high as 4.4 Lsponge m−2. We estimated an average areal DIN contribution from total sponge biomass of 0.59 ± 0.28 mmol N m−2 d−1. However, calculated fluxes from the 59 stations exhibited significant spatial variability associated with changes in the size and species composition of the sponge community; peak N fluxes reached 3.5 ± 0.9 mmol N m−2 d−1 in areas with large populations of high microbial abundance sponges. The average flux from the sponge community was the largest of the estimated sources of DIN to Mystery Basin, representing roughly half of the overall N sourcing. This N satisfied more than half of the demand by primary productivity. These results indicate that sponges are important sources of inorganic N to Florida Bay environments

Microbial activity in surficial sediments overlying acoustic wipeout zones at a Gulf of Mexico cold seep

Down core concentration gradients of dissolved methane and sulfate; isotope gradients of methane, dissolved inorganic carbon, and authigenic carbonate; and organic matter elemental ratios are incorporated into a vent evolution model to describe spatial and temporal variability of sedimentary microbial activity overlying acoustic wipeout zones at Mississippi Canyon (MC) 118, Gulf of Mexico. We tested the hypothesis that these zones indicate areas where sediments are exposed to elevated fluid flux and therefore should contain saturated methane concentrations and enhanced microbial activity from sulfate reduction (SR), anaerobic oxidation of methane (AOM), and methanogenesis (MP). Thirty surficial cores (between 22 and 460 cm deep) were collected from sediments overlying and outside the wipeout zones and analyzed for pore water and solid phase constituents. Outside the wipeout zones, sulfate and methane concentrations were similar to overlying-water values and did not vary with depth; indicating low microbial activity. Above the wipeouts, nine cores showed moderate activity with gently sloping sulfate and methane concentration gradients, methane concentrations <20 μM, and isotope depth gradients indicative of organic matter oxidation. In stark contrast to this moderate activity, four cores showed high microbial activity where sulfate concentrations were depleted by ∼50 cm below seafloor, maximum methane concentrations in the decompressed cores were above 4 mM, and down core profiles of δ13C-CH4 and δ13C-dissolved inorganic carbon (DIC) indicated distinct depth zones of SR, AOM, and MP. Bulk organic matter analysis suggested that the high activity was supported by an organic source that was enriched in carbon (C:N ∼15) and depleted in d15N and δ13C compared to other activity groups, possibly due to the influx of petroleum or chemosynthetically fixed carbon. Within high activity cores, the δ13C-DIC values were similar to the δ13C-CaCO3 values, a result expected for authigenic carbonate recently precipitated. However, these values were dissimilar in moderate activity cores, suggesting that microbial activity was higher in the past. This study provides evidence that the fluid flux at MC 118 varies over time and that the microbial activity responds to such variability. It also suggests that sediments overlying wipeout zones are not always saturated with respect to methane, which has implications for the formation and detection of gas hydrate

Spatial and seasonal variations in the stable carbon isotopic composition of methane in stream sediments of eastern Amazonia

The stable carbon isotopic composition of methane (δ13 C-CH4) gas bubbles formed in the sediments of three Amazonian streams was determined over a 5-yr period. The study sites were two ' 'várzea' floodplain (Açu and Maicá) and one 'terra-firme' (Jamaraquá) streams. The δ 13C of sedimentary organic matter (SOM) from the surrounding vegetation and bottom sediments were also determined. The mean δ13C value of SOM was lower in the terra-firme (-29.6‰) than in the várzea stream (-23.8‰) as a result of less C4 plant deposition in the former. The δ 13C-CH4 values varied systematically both seasonally and spatially among the sites during all 5 yr of the study, in association with changes during hydrologic cycle. Overall, the variation in values of δ 13C-CH4 during the high water phase covered a narrower range of values, -63 to -56‰. Contrastively, during the low water phase the δ 13C-CH4 values for várzea and terra-firme streams are different and are in direct opposition. At this phase, the δ13C-CH4 at terra-firme stream is at least 20‰ depleted of 13C compared to várzea streams. Changes in organic matter sources, water levels and associated microbial degradation processes control the observed seasonal and spatial variations in net stable carbon isotopic composition of methane