Personal style of the therapist and personality dimensions in a sample of Argentinian therapists

The Personal Style of the Therapist (PST) is an important aspect to assess in the therapeutic process. Previous research has explored therapist's profiles and their differences according to a variety of theoretical orientations, as well as the association between these profiles and personality traits. This study aims to put together these lines of research through a cluster analysis. The specific goals were to describe the profiles of therapists with respect to the functions of the PST and personality traits; and to analyze whether the professionals' profiles and their theoretical orientations were related. The sample was comprised of 546 Argentinian therapists. The Personal Style of the Therapist Questionnaire (PST-Q) and the Big Five Inventory (BFI) were used as measuring instruments. Using the methodology of hierarchical analysis based on a cluster analysis integrating the five factors of the PST, a four-cluster solution was confirmed to be the most satisfactory. Subsequently, a cluster analysis of k-means based on the five factors of each test (PST-Q and BFI) was performed. Finally, the relationship between the clusters and theoretical orientation and years of clinical practice was assessed, rendering positive outcomes. The results are discussed based on previous research studies and the significance of studying the therapist's personal variables and their connection with psychotherapy efficacy.Fil: Casari, Leandro Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Ison, Mirta Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Gómez, Beatriz Margarita María. Fundación Aigle. Sede Central Buenos Aires. Departamento de Investigación. Investigación Empirica En Psicoterapia; Argentin

Therapist personal styles: A comparison between professionals working in drug addiction institutions and professionals working in the general clinical area

Los objetivos de este artículo fueron: a) describir el perfil de Estilo Personal del Terapeuta (EPT) en dos grupos de psicólogos clínicos: uno que trabaja en instituciones de tratamiento de adicciones y otro no abocado específicamente a este campo; b) comparar el posible efecto del enfoque teórico y los años de experiencia profesional sobre el EPT en cada grupo. Para ello se trabajó con una muestra de 429 psicólogos clínicos de Argentina divididos en dos grupos: Grupo Adicciones (n= 102) y Grupo Clínico (n= 327). Como instrumento se empleó el Cuestionario de Estilo Personal del Terapeuta. A nivel de resultados se obtuvieron diferencias significativas en la función de Involucración (p< .05), donde los terapeutas de adicciones obtuvieron puntajes superiores. Asimismo, se observaron diferencias entre ambos grupos de terapeutas según el enfoque teórico y correlaciones significativas con los años de experiencia profesional.The objectives of this study were: (a) to describe the profile of the Therapist’s Personal Style (TPS) in two groups of clinical psychologists: one group working in drug addict institutions and the other group dedicated to general clinical treatment; (b) to compare the possible effect of the theoretical framework and the years of experience on the TPS in each group. For this purpose, a sample of 429 clinical psychologists from Argentina was divided into two groups: Addictions Group (n = 102) and Clinical Group (n = 327). The Therapeutic Personal Style Questionnaire was used as instrument. As a result, there were significant differences in the Engagement function (p < .05), higher among the therapists of drug addict patients. Likewise, differences between both groups of therapists were observed according to the theoretical approach as well as significant correlations with years of professional experience.Fil: Casari, Leandro Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Ison, Mirta Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Gómez, Beatriz. Fundación Aigle. Sede Central Buenos Aires. Departamento de Investigación. Investigación Empirica En Psicoterapia; Argentin

A specific N-terminal extension of the 8 kDa domain is required for DNA end-bridging by human Polµ and Polλ

Human DNA polymerases mu (Polµ) and lambda (Polλ) are X family members involved in the repair of double-strand breaks in DNA during non-homologous end joining. Crucial abilities of these enzymes include bridging of the two 3′ single-stranded overhangs and trans-polymerization using one 3′ end as primer and the other as template, to minimize sequence loss. In this context, we have studied the importance of a previously uncharacterised sequence (‘brooch’), located at the N-terminal boundary of the Polß-like polymerase core, and formed by Tyr141, Ala142, Cys143, Gln144 and Arg145 in Polµ, and by Trp239, Val240, Cys241, Ala242 and Gln243 in Polλ. The brooch is potentially implicated in the maintenance of a closed conformation throughout the catalytic cycle, and our studies indicate that it could be a target of Cdk phosphorylation in Polµ. The brooch is irrelevant for 1 nt gap filling, but of specific importance during end joining: single mutations in the conserved residues reduced the formation of two ended synapses and strongly diminished the ability of Polµ and polymerase lambda to perform non-homologous end joining reactions in vitro

New biostratigraphic data from the Callovian-Oxfordian La Manga Formation, Neuquén Basin, Argentina: Evidence from an ammonite condensed level

The La Manga Formation constitutes most of the sedimentary record of the Callovian-Oxfordian in the Neuquén Basin. This stratigraphic unit represents the middle part of the Lotena Mesosequence, which is dominated by carbonates with interbedded shales, marls, and occasional sandstones. Based on detailed stratigraphic work in the stratotype section (La Manga Creek, Mendoza), the depositional environments and the sea-level history of the La Manga Formation have been interpreted. Petrographic and field observations led to identification of nine facies distributed in two informal units. Unit 1 was deposited on a outer ramp setting, while deposition of unit 2 took place in the intertidal-supratidal environments. A condensed level close to the base of unit 1 has yielded Rehmannia sp., Rehmannia cf. paucicostata (Tornq.) and Homoeoplanulites sp., from the Lower Callovian Bodenbenderi-Proximum Zones, and Peltoceratoides sp. and Rursiceras sp. from the Upper Callovian and also in the Lower Oxfordian Peltoceratoides-Parawedekindia Zone. The condensed level is overlain by a Middle Oxfordian succession characterized by Perisphinctes (?Arisphinctes) sp., Perisphinctes (?Kranaosphinctes) sp., Mirosphinctes sp., Perisphinctes (?Antilloceras) cf. prophetae Gygi & Hill., Perisphinctes (?Otosphinctes) sp., Perisphinctes (?Subdiscosphinctes) sp., and Perisphinctes (?Kranaosphinctes) cf. decurrens (Buck.) of the Perisphinctes-Araucanites Zone, which was correlated with the upper part of the Cordatum Standard Zone to the Transversarium Standard Zone, and probably to the lower part of the Bifurcatus Standard Zone. Unit 2 has yielded small Mirosphinctes sp., indicating an Oxfordian age. The sharp contact between the outer ramp facies of unit 1 and the overlying intertidal-supratidal facies of unit 2 can be interpreted as the result of an abrupt fall (forced regression) of the relative sea level during the end of Middle Oxfordian or Upper Oxfordian. These results could be used for comparison with other localities in the Neuquén Basin providing additional data for Lower Callovian-Middle Oxfordian deposits

Occurrence of Coxiella burnetii in wild lagomorphs and their ticks in Spanish Mediterranean ecosystems

Background: Coxiella burnetii, the causative agent of Q fever, is a zoonotic multi-host vector-borne pathogen of major public health importance. Although the European Food Safety Authority has recently made the monitoring of this bacterium in wildlife a priority, the role of wild lagomorphs in the transmission and maintenance of C. burnetii is poorly understood. Aims: The aims of this study were to determine the prevalence and risk factors associated with C. burnetii circulation in European wild rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis) and to assess the presence of this pathogen in ticks that feed on them in Mediterranean ecosystems in Spain, the country with the highest number of reported cases of Q fever in Europe. Methods: A total of 574 spleen samples were collected from 453 wild rabbits and 121 Iberian hares, and 513 ticks (processed in 120 pools) between the 2017/2018 and 2021/2022 hunting seasons. Results: C. burnetii DNA was detected in 103 (17.9%; 95% CI: 14.8–21.1) of the 574 wild lagomorphs tested. By species, prevalence was 16.3% (74/453; 95% CI: 12.9–19.7) in the European wild rabbit and 24.0% (29/121; 95% CI: 16.4–31.6) in the Iberian hare. At least one positive lagomorph was found on 47.9% of the 96 hunting estates sampled and in every hunting season since 2018/2019. Two risk factors associated with C. burnetii infection were as follows: outbreak of myxomatosis on the hunting estate in the month prior to sampling and high tick abundance observed by gamekeepers on the hunting estate. C. burnetii DNA was also found in 33 of the 120 (27.5%; 95% CI: 19.5–35.5) tick pools tested. The pathogen was detected in 66.7% (4/6), 29.2% (26/89) and 21.4% (3/14) of Haemaphysalis hispanica, Rhipicephalus pusillus and Hyalomma lusitanicum pools respectively. Conclusions: This study provides new epidemiological data on C. burnetii in European wild rabbits and is the first survey on this zoonotic pathogen performed in Iberian hares. Our results indicate widespread endemic circulation of C. burnetii and highlight the importance of both wild lagomorph species as natural reservoirs of this zoonotic bacterium in Mediterranean ecosystems in southern Spain, which may be of public and animal health concern. The high prevalence and wide diversity of positive tick species suggest the possible role of ticks in the epidemiological cycle of C. burnetii, with the potential risk of transmission to sympatric species, including humans

Perioperative anemia management in colorectal cancer patients: A pragmatic approach.

REVIEW;Anemia, usually due to iron deficiency, is highly prevalent among patients with colorectal cancer. Inflammatory cytokines lead to iron restricted erythropoiesis further decreasing iron availability and impairing iron utilization. Preoperative anemia predicts for decreased survival. Allogeneic blood transfusion is widely used to correct anemia and is associated with poorer surgical outcomes, increased post-operative nosocomial infections, longer hospital stays, increased rates of cancer recurrence and perioperative venous thromboembolism. Infections are more likely to occur in those with low preoperative serum ferritin level compared to those with normal levels. A multidisciplinary, multimodal, individualized strategy, collectively termed Patient Blood Management, minimizes or eliminates allogeneic blood transfusion. This includes restrictive transfusion policy, thromboprophylaxis and anemia management to improve outcomes. Normalization of preoperative hemoglobin levels is a World Health Organization recommendation. Iron repletion should be routinely ordered when indicated. Oral iron is poorly tolerated with low adherence based on published evidence. Intravenous iron is safe and effective but is frequently avoided due to misinformation and misinterpretation concerning the incidence and clinical nature of minor infusion reactions. Serious adverse events with intravenous iron are extremely rare. Newer formulations allow complete replacement dosing in 15-60 min markedly facilitating care. Erythropoiesis stimulating agents may improve response rates. A multidisciplinary, multimodal, individualized strategy, collectively termed Patient Blood Management used to minimize or eliminate allogeneic blood transfusion is indicated to improve outcomes.Ye

Mediterranean Heathland as a Key Habitat for Fire Adaptations: Evidence from an Experimental Approach

Some fire ecology studies that have focused on garrigue-like vegetation suggest a weak selective pressure of fire in the Mediterranean Basin compared to other Mediterranean-type regions. However, fire-prone Mediterranean heathland from the western end of the Mediterranean Basin has been frequently ignored in the fire ecology literature despite its high proportion of pyrogenic species. Here, we explore the evolutionary ecology of seed traits in the generalist rockrose Cistus salviifolius L. (Cistaceae) aiming to ascertain the role of the Mediterranean heathland for fire adaptations in the Mediterranean Region. We performed a germination experiment to compare the relationship of seed size to (i) heat-stimulated germination, (ii) dormancy strength, and (iii) heat survival in plants from 'high-fire' heathland vs. 'low-fire' coastal shrubland. Germination after heat-shock treatment was higher in large seeds of both 'high-fire' and 'low-fire' habitats. However, dormancy was weaker in small seeds from 'low-fire' habitats. Finally, seed survival to heat shock was positively related to seed size. Our results support that seed size is an adaptive trait to fire in C. salviifolius, since larger seeds had stronger dormancy, higher heat-stimulated germination and were more resistant to heat shock. This seed size-fire relationship was tighter in 'high-fire' Mediterranean heathland than 'low-fire' coastal shrubland, indicating the existence of di fferential fire pressures and evolutionary trends at the landscape scale. These findings highlight the Mediterranean heathland as a relevant habitat for fire-driven evolution, thus contributing to better understand the role of fire in plant evolution within the Mediterranean region

Simulacro de Congreso Científico. Aplicación en el Grado de Enfermería.

Introduction. European Higher Education Space proposes new educational methodologies geared to the acquisition of skills by students. In this paper a mock scientific Congress is presented as one of these tools. Aim. The aim of this study was to analyze the level of acceptance and increased communication skills of a group of nursing students in a mock Scientific Congress. Results. 84 students participated in the experiment. A large majority of them showed objective improvement of their communicative competence levels after the completion of the mock. About 70% of them evaluated the experience positively. Conclusion. Mock Scientific Congress behaves as a useful tool in the development of communication skills of students.Introducción. El Espacio Europeo de Educación Superior propone nuevas metodologías educativas enfocadas hacia la adquisición de competencias por parte de los estudiantes. En este trabajo el simulacro de Congreso científico es presentado como una de estas herramientas.Objetivo. El objetivo de este estudio fue analizar el nivel de aceptación y el aumento de las competencias comunicativas de un grupo de alumnos del Grado de Enfermería que participaron en un simulacro de Congreso Científico.Material y método. Se realizó una comparación (pre-simulacro y simulacro) de de las competencias comunicativas cuantificadas mediante rúbrica. Los participantes cumplimentaron una encuesta de satisfacción con la experiencia.Resultados. 84 alumnos participaron en la experiencia. Una gran mayoría de ellos mostró una mejoría objetiva de sus niveles de competencia comunicativa tras la realización del simulacro. Alrededor del 70% de ellos valoró de forma positiva la experiencia.Conclusiones. El simulacro de Congreso Científico se comporta como una herramienta útil en el desarrollo de las competencias comunicativas de los alumnos

Terminal restriction fragment length polimorphism (T-RFLP) : una herramienta útil para valorar la dinámica y la diversidad de la población microbiana en aves y cerdos

El ecosistema intestinal es un medio complejo y dinámico que puede verse afectado puntualmente por numerosos factores propios del individuo pero también por numerosos factores ambientales o externos (como puede ser la dieta). La necesidad de conocer de una forma rápida y reproducible cómo es la dinámica de la población bacteriana, así como su estructura está siendo posible gracias al desarrollo de técnicas moleculares aplicadas como DGGE (Denaturing Gradient Gel Electrophoresis)1, TGGE (Temperatura Gradient Gel Electrophoresis)2, SSCP (Single Strand Conformation Polimorphism)3 y T-RFLP (Terminal Restriction Fragment Length Polimorphism)4. Estas técnicas moleculares aprovechan la característica de universalidad del gen que codifica para el 16S ARNr, presente en prácticamente la totalidad de las especies bacterianas. La presencia de secuencias altamente conservadas hace posible el diseño de primers o cebadores que permitan la amplificación mediante PCR del total de la población bacteriana de la muestra. Son las regiones variables (polimórficas) del gen que codifica el 16S ARNr las que se aprovechan para la diferenciación. En el T-RFLP, estas diferencias entre especies o géneros bacterianos se ponen de manifiesto tras la digestión con endonucleasas de restricción del producto de PCR marcado. Únicamente el fragmento del extremo terminal será visible por electroforesis capilar gracias al mareaje fluorescente de uno de los cebadores. El perfil que se genera (electroferograma) nos permite obtener información del número de fragmentos (riqueza), de su tamaño en pares de bases (bp) (para una posible inferencia de la especie bacteriana presente) y de su altura (lo que puede orientar sobre la importancia de un determinado grupo bacteriano dentro de una muestra), siempre sin olvidar las desviaciones inherentes propias de la PCR. Es posible también calcular la frecuencia de detección de un determinado pico (especie) respecto al total de muestras así como construir con ayuda de programas informáticos matrices de similitud o dendogramas. Empleando herramientas disponibles en la red como: TAP-tRFLP del software Ribosomal Database Project II o ISPaR del software MiCA (Microbial Community Analysis) es posible hacer una asignación teórica de la especie o género bacteriano a cada uno de los picos en base a las secuencias depositadas por otros autores. Sin embargo un mismo pico puede tener su origen en distintas especies. Disponer alternativamente de secuencias propias obtenidas tras la clonación del 16S ARN de muestras de nuestros animales nos permite hacer una asignación teórica más firme, considerando únicamente aquellas especies mayoritarias encontradas