Using virtual environments to improve the realism of choice experiments: a case study about coastal erosion management

Choice experiment surveys are commonly used to assess the general public's willingness to pay for different levels of environmental quality. However, respondents need to understand what they are valuing or they will make potentially wrong assumptions based on different experiences and frames of reference. Three-dimensional computer generated models or Virtual Environments (VE) have so far seen little use in economics research, probably due to the complexity and cost of developing and delivering them to study participants. The few studies that have used them find that VE are superior to static image presentations in helping people evaluate complex data. For this study we developed virtual environments for a choice experiment about coastal erosion management using free, easy-to-use software and Google Earth© satellite imagery and presented these to respondents as video tours. Our results indicate that the VE treatment reduced choice error, reduced left-right bias and improved respondent engagement and retention when compared with static images. There were also differences in WTP between the two groups

Infectious HIV-1 assembles in late endosomes in primary macrophages

Although human immunodeficiency virus type 1 (HIV-1) is generally thought to assemble at the plasma membrane of infected cells, virions have been observed in intracellular compartments in macrophages. Here, we investigated virus assembly in HIV-1–infected primary human monocyte-derived macrophages (MDM). Electron microscopy of cryosections showed virus particles, identified by their morphology and positive labeling with antibodies to the viral p17, p24, and envelope proteins, in intracellular vacuoles. Immunolabeling demonstrated that these compartments contained the late endosomal marker CD63, which was enriched on vesicles within these structures and incorporated into the envelope of budding virions. The virus-containing vacuoles were also labeled with antibodies against LAMP-1, CD81, and CD82, which were also incorporated into the viral envelope. To assess the cellular source of infectious viruses derived from MDM, virus-containing media from infected cells were precipitated with specific antibodies. Only antibodies against antigens found in late endosomes precipitated infectious virus, whereas antibodies against proteins located primarily on the cell surface did not. Our data indicate that most of the infectious HIV produced by primary macrophages is assembled on late endocytic membranes and acquires antigens characteristic of this compartment. This notion has significant implications for understanding the biology of HIV and its cell–cell transmission

The intracellular plasma membrane-connected compartment in the assembly of HIV-1 in human macrophages

Background In HIV-infected macrophages, newly formed progeny virus particles accumulate in intracellular plasma membrane-connected compartments (IPMCs). Although the virus is usually seen in these compartments, it is unclear whether HIV assembly is specifically targeted to IPMCs or whether some viruses may also form at the cell surface but are not detected, as particles budding from the latter site will be released into the medium. Results To investigate the fidelity of HIV-1 targeting to IPMCs compared to the cell surface directly, we generated mutants defective in recruitment of the Endosomal Sorting Complexes Required for Transport (ESCRT) proteins required for virus scission. For mutants unable to bind the ESCRT-I component Tsg101, HIV release was inhibited and light and electron microscopy revealed that budding was arrested. When expressed in human monocyte-derived macrophages (MDM), these mutants formed budding-arrested, immature particles at their assembly sites, allowing us to capture virtually all of the virus budding events. A detailed morphological analysis of the distribution of the arrested viruses by immunofluorescence staining and confocal microscopy, and by electron microscopy, demonstrated that HIV assembly in MDMs is targeted primarily to IPMCs, with fewer than 5 % of budding events seen at the cell surface. Morphometric analysis of the relative membrane areas at the cell surface and IPMCs confirmed a large enrichment of virus assembly events in IPMCs. Serial block-face scanning electron microscopy of macrophages infected with a budding-defective HIV mutant revealed high-resolution 3D views of the complex organisation of IPMCs, with in excess of 15,000 associated HIV budding sites, and multiple connections between IPMCs and the cell surface

KCNQ/M currents in sensory neurons: Significance for pain therapy

Neuronal hyperexcitability is a feature of epilepsy and both inflammatory and neuropathic pain. M currents [I-K(M)] play a key role in regulating neuronal excitability, and mutations in neuronal KCNQ2/3 subunits, the molecular correlates of I-K(M), have previously been linked to benign familial neonatal epilepsy. Here, we demonstrate that KCNQ/M channels are also present in nociceptive sensory systems. I-K(M) was identified, on the basis of biophysical and pharmacological properties, in cultured neurons isolated from dorsal root ganglia (DRGs) from 17-d-old rats. Currents were inhibited by the M-channel blockers linopirdine (IC50, 2.1 muM) and XE991 (IC50, 0.26 muM) and enhanced by retigabine (10 muM). The expression of neuronal KCNQ subunits in DRG neurons was confirmed using reverse transcription-PCR and single-cell PCR analysis and by immunofluorescence. Retigabine, applied to the dorsal spinal cord, inhibited C and Adelta fiber-mediated responses of dorsal horn neurons evoked by natural or electrical afferent stimulation and the progressive "windup" discharge with repetitive stimulation in normal rats and in rats subjected to spinal nerve ligation. Retigabine also inhibited responses to intrapaw application of carrageenan in a rat model of chronic pain; this was reversed by XE991. It is suggested that I-K(M) plays a key role in controlling the excitability of nociceptors and may represent a novel analgesic target

Pedagogical affect, student interest, and learning performance

Using a sample of more than 1000 students, this study reveals that students’ perceived learning depends directly on their interest, pedagogical affect, and their learning performance and indirectly on the student–instructor interaction, the instructor's responsiveness, course organization, the instructor's likeability/concern, and the student's learning performance. Likeability/concern indirectly affects student interest by influencing learning performance. The results yield recommendations for schools, department heads, and university administrators.This research was funded by a research grant from NOVA EGIDE to Luís Filipe Lages. The authors acknowledge the two anonymous JBR reviewers for their feedback on a previous version of the article

Accretion disc dynamics in extreme mass ratio compact binaries

An analysis is presented of a numerical investigation of the dynamics and geometry of accretion discs in binary systems with mass ratios q < 0.1, applicable to ultra-compact X-ray binaries, AM CVn stars and very short period cataclysmic variables. The steady-state geometry of the disc in the binary reference frame is found to be quite different from that expected at higher mass ratios. For q ~ 0.1, the disc takes on the usual elliptical shape, with the major axis aligned perpendicular to the line of centres of the two stars. However, at smaller mass ratios the elliptical gaseous orbits in the outer regions of the disc are rotated in the binary plane. The angle of rotation increases with gas temperature, but is found to vary inversely with q. At q = 0.01, the major axis of these orbits is aligned almost parallel to the line of centres of the two stars. These effects may be responsible for the similar disc structure inferred from Doppler tomography of the AM CVn star GP Com (Morales-Rueda et al. 2003), which has q = 0.02. The steady-state geometry at low mass ratios is not predicted by an inviscid, restricted three-body model of gaseous orbits; it is related to the effects of tidal-viscous truncation of the disc near the Roche lobe boundary. Since the disc geometry can be inferred observationally for some systems, it is proposed that this may offer a useful diagnostic for the determination of mass ratios in ultra-compact binaries.Comment: 17 pages, 9 figures, 7 in colour. Accepted for publication in MNRAS. Plain article formatting to get round arXiv problems with mn2e.st

Evaluation of BacLite Rapid MRSA, a rapid culture based screening test for the detection of ciprofloxacin and methicillin resistant S. aureus (MRSA) from screening swabs

BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. The need for accurate and rapid screening methods to detect MRSA carriers has been clearly established. The performance of a novel assay, BacLite Rapid MRSA (Acolyte Biomedica, UK) for the rapid detection (5 h) and identification of hospital associated ciprofloxacin resistant strains of MRSA directly from nasal swab specimens was compared to that obtained by culture on Mannitol salt agar containing Oxacillin (MSAO) after 48 h incubation. RESULTS: A total of 1382 nasal screening swabs were tested by multiple operators. The BacLite Rapid MRSA test detected 142 out of the 157 confirmed MRSA that were detected on MSAO giving a diagnostic sensitivity of 90.4, diagnostic specificity of 95.7% and a negative predictive value of 98.7%. Of the 15 false negatives obtained by the BacLite Rapid MRSA test, seven grew small amounts (< 10 colonies of MRSA) on the MSAO culture plate and five isolates were ciprofloxacin sensitive. However there were 13 confirmed BacLite MRSA positive samples, which were negative by the direct culture method, probably due to overgrowth on the MSAO plate. There were 53 false positive results obtained by the BacLite Rapid MRSA test at 5 h and 115 cases where MRSA colonies were tentatively identified on the MSAO plate when read at 48 h, and which subsequently proved not to be MRSA. CONCLUSION: The Baclite MRSA test is easy to use and provides a similar level of sensitivity to conventional culture for the detection of nasal carriage of MRSA with the advantage that the results are obtained much more rapidly

Doppler tomography as a tool for detecting exoplanet atmospheres

High-resolution Doppler spectroscopy is a powerful tool for identifying molecular species in the atmospheres of both transiting and non-transiting exoplanets. Currently, such data is analysed using cross-correlation techniques to detect the Doppler shifting signal from the orbiting planet. In this paper we demonstrate that, compared to cross-correlation methods currently used, the technique of Doppler tomography has improved sensitivity in detecting the subtle signatures expected from exoplanet atmospheres. This is partly due to the use of a regularizing statistic, which acts to suppress noise, coupled to the fact that all the data is fit simultaneously. In addition, we show that the technique can also effectively suppress contanimating spectral features that may arise due to overlapping lines, repeating line patterns, or the use of incorrect linelists. These issues can confuse conventional cross-correlation approaches, primarily due to aliasing issues inherent in such techniques, whereas Doppler tomography is less susceptible to such effects. In particular, Doppler tomography shows exceptional promise for simultaneously detecting multiple line species (e.g. isotopologues), even when there are high contrasts between such species – and far outperforms current CCF analyses in this respect. Finally, we demonstrate that Doppler tomography is capable of recovering molecular signals from exoplanets using real data, by confirming the strong detection of CO in the atmosphere of τ Boo b. We recover a signal with a planetary radial velocity semi-amplitude Kp = 109.6 ± 2.2 km s−1, in excellent agreement with the previously reported value of 110.0 ± 3.2 km s−1

High Spatial Resolution Mid-infrared Observations of Three Seyfert Galaxies

Images at 12.5 microns of nuclei of three nearby Seyfert galaxies -- NGC 1275, NGC 4151 and NGC 7469 -- have been obtained with the Keck 10-m Telescope. NGC 7469 is resolved and deconvolution delineates a structure <0.04"x0.08" or <13x26 pc at a position angle of 135deg. From a comparison with structure seen at millimeter wavelengths, this structure is interpreted as a disk aligned with the molecular gas in the central few hundred parsecs of the galaxy. NGC 1275 and NGC 4151 are not resolved; limits on the sizes of these nuclei are 0.08" and 0.16", corresponding to physical spatial scales of 28 and 10 pc. The lower limits to the brightness temperatures implied by these size limits and the measured flux densities are within ~50K of the 12 micron to 25 micron color temperatures of these systems as inferred from IRAS observations. The angular size limits are within a factor of 2.5 of the sizes required to spatially resolve thermal emission from dust heated by a central luminosity source. These sizes preclude significant contributions to the nuclear infrared emission from star forming regions.Comment: 23 pages, 7 figures, A.J. in pres