Einfluss neuromyotrop wirkender Substanzen auf die Ejakulation des Ebers anhand spermatologischer und biochemischer Parameter

Available from: Zentralstelle fuer Agrardokumentation und -information (ZADI), Villichgasse 17, D-53177 Bonn / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Quantification of Y-bearing spermatozoa of beef cattle throught ciscontinuous Percoll gradient centrifugation using fluorescent in situ hybridization

An appropriated procedure for proportion of Y-bearing spermatozoa is needed, especially to monitor sperm separation techniques. In this study was aimed to determine the Y-bearing spermatozoa proportion in bull semen through pre and post 6 layers discontinuous Percoll centrifugation and by using fluorescent in situ hybriddization (FISH) technique with digoxigenin (Dig)-labeled bovine Y-specific probe, which prepared by polymerase chain reaction (PCR) using BC1.2 primers. This results fould that the metaphase male lymphocyte was clearly shown green-yellow fluorescence spot on the short arm of Y-chromosome, whereas an interphase male lymphocyte showed this signal within nuclei, in conversely, both interphase and metaphase female lymphocytes were not shown this signal. An average interphase male lymphocyte obviously showed the signal 96.8%. The proportion of Y-bearing spermatozoa in semen pre and post discontinuous Percoll centrifugation were 48.78% and 41.68%, respectively (P>0.05). These results indicated that FISH protocol with Dig-labeled bovine Y-specific probe could be used to mornitor the proportion of Y-bearing spermatozoa through discontinuous Percoll centrifugation in bull semen which after sperm separation, the proportion of Y-chromosome trend to be not significantly reduced

Effect of methionine levels on production performance, triglyceride and non-esterified fatty acid in laying hens

This study was conducted to evaluate the effect of methionine (Met) on egg production, liver triglyceride and blood free fatty acid (non-esterified fatty acid; NEFA) in laying hens aged 21 to 32 weeks old by using completely randomized design. Low-protein diet (14% CP) containing Met at 0.28 (unsupplemented group), 0.30, 0.38 or 0.44% of diet were used. The results showed that egg production and egg mass tended to increase, while feed and energy efficiency were significantly improved when Met levels increased (p<0.05).Liver weight and liver triglyceride were significantly increased by the Met supplementation, but there was no evidence of fatty liver syndrome. In addition, NEFA was slightly decreased but body weight tended to increase due to adding Met, although statistical differences were not seen. In conclusion, the improvement of egg production caused by an increase of Met levels may be closely related with the changing proportion of lipogenesis and lipolysis due to an improving amino acid balance

Effects of evaporative cooling on reproductive performance and milk production of dairy cows in hot wet conditions

Fourteen animals of second and third lactationof Thai Friesian crossbred cows (87.5% Friesian × 12.5%Bos indicus) located at Sakol Nakhon Research andBreeding Centre, Department of Livestock Development,Ministry of Agriculture and Cooperatives, were dividedrandomly into two groups of seven each to evaluate theeffects of evaporative cooling on reproductive and physiologicaltraits under hot, humid conditions. Resultsindicated that installation of evaporating cooling in theopen shed gave a further improvement in ameliorating heatstress in dairy cows in hot-wet environments by utilisingthe low humidity conditions that naturally occur during theday. The cows housed in an evaporatively cooled environmenthad both a rectal temperature and respiration rate(39.09⁰C, 61.39 breaths/min, respectively) significantlylower than that of the non-cooled cows (41.21⁰C;86.87 breaths/min). The former group also had highermilk yield and more efficient reproductive performance(pregnancy rate and reduced days open) than the lattergroup. It is suggested that the non-evaporatively cooledcows did not gain benefit from the naturally lower heatstress during night time

Effect of supplementary lighting on eating behaviour by corralled swamp buffalo (Bubalus bubalis ) heifers in Thailand

Sixteen 14-month-old swamp buffalo (Bubalus bubalis) heifers were used to study the effect of supplementary lighting on eating time, number of meals and meal duration and growth performance. Eightheifers were allocated to a natural photoperiod regime, receiving approximately 12 h of daylight, (control treatment) and eight heifers were allocated to a supplementary lighting regime, receiving an additional 6 h of artificial light during the night, (light supplemented treatment) using a cross-over design. Rice straw wasoffered ad libitum and commercial concentrate was also offered approximately 1.5 kg/animal/day. Supplementary lighting was provided by eight 60 W white fluorescent tubes placed approximately 2.5 m above theground under the roof. Supplementary lighting did not significantly effect eating behaviour, daily intake or live weight gain. It is concluded that the performance of corralled buffalo heifers cannot be improved by the provision of supplementary lighting