Trombocitopatia e coagulopatia em bovinos associadas à infestação do carrapato Rhipicephalus (Boophilus) microplus

06

Ornithobacterium rhinotracheale : genotipificação e sorotipificação de isolados brasileiros

04

A genetic and virulence characterization of Brazilian strains of Mycoplasma hyopneumoniae

Mycoplasma hyopneumoniae (M. hyopneumoniae) is considered the primary causative agent of porcine enzootic pneumonia (EP), a chronic contagious respiratory disease that causes economic losses. Obtaining new pathogenic isolates and studying the genome and virulence factors are necessary. This study performed a complete sequencing analysis of two Brazilian strains, UFV01 and UFV02, aiming to characterize the isolates in terms of the virulence factors and sequence type. The complete genome analysis revealed the main virulence genes (mhp385, mhp271, MHP_RS03455, p102, p97, p216, MHP_RS00555, mhp107) and ST-123, the presence of three toxin-related genes (tlyC, PLDc_2 and hcnC), and some genetic groups specific to these two isolates. Subsequently, the pathogenicity of the isolates was evaluated via an experimental infection conducted in a swine model. The study was divided into three groups, namely a negative control group (n = 4) and two test groups (n = 8), totaling 20 animals. They were challenged at 35 days of age with 107 CCU (Color Changing Units) M. hyopneumoniae via the intratracheal route. The UFV01 group showed earlier and higher seroconversion (IgG) (100%), while only 50% of the UFV02 group seroconverted. The same trend was observed when analyzing the presence of IgA in the bronchoalveolar lavage fluid (BALF) at 35 days post-infection (dpi). The UFV01 group had a mean macroscopic lesion score of 11.75% at 35 dpi, while UFV02 had 3.125%. Microscopic lesions were more severe in the UFV01 group. Based on laryngeal swab samples evaluated by qPCR, and the detection began at 14 days. The UFV01 group showed 75% positivity at 14 dpi. The UFV02 group also started excreting at 14 dpi, with a positivity rate of 37.5%. The results indicate that the UFV01 isolate exhibits higher virulence than UFV02. These findings may aid in developing new vaccines and diagnostic kits and establishing experimental models for testing

Health related quality of life measure in systemic pediatric rheumatic diseases and its translation to different languages: an international collaboration

Background: Rheumatic diseases in children are associated with significant morbidity and poor health-related quality of life (HRQOL). There is no health-related quality of life (HRQOL) scale available specifically for children with less common rheumatic diseases. These diseases share several features with systemic lupus erythematosus (SLE) such as their chronic episodic nature, multi-systemic involvement, and the need for immunosuppressive medications. HRQOL scale developed for pediatric SLE will likely be applicable to children with systemic inflammatory diseases.Findings: We adapted Simple Measure of Impact of Lupus Erythematosus in Youngsters (SMILEY (c)) to Simple Measure of Impact of Illness in Youngsters (SMILY (c)-Illness) and had it reviewed by pediatric rheumatologists for its appropriateness and cultural suitability. We tested SMILY (c)-Illness in patients with inflammatory rheumatic diseases and then translated it into 28 languages. Nineteen children (79% female, n= 15) and 17 parents participated. the mean age was 12 +/- 4 years, with median disease duration of 21 months (1-172 months). We translated SMILY (c)-Illness into the following 28 languages: Danish, Dutch, French (France), English (UK), German (Germany), German (Austria), German (Switzerland), Hebrew, Italian, Portuguese (Brazil), Slovene, Spanish (USA and Puerto Rico), Spanish (Spain), Spanish (Argentina), Spanish (Mexico), Spanish (Venezuela), Turkish, Afrikaans, Arabic (Saudi Arabia), Arabic (Egypt), Czech, Greek, Hindi, Hungarian, Japanese, Romanian, Serbian and Xhosa.Conclusion: SMILY (c)-Illness is a brief, easy to administer and score HRQOL scale for children with systemic rheumatic diseases. It is suitable for use across different age groups and literacy levels. SMILY (c)-Illness with its available translations may be used as useful adjuncts to clinical practice and research.Rutgers State Univ, Robert Wood Johnson Med Sch, New Brunswick, NJ 08903 USARutgers State Univ, Child Hlth Inst New Jersey, New Brunswick, NJ 08901 USAHosp Special Surg, New York, NY 10021 USAUniv Michigan, Ann Arbor, MI 48109 USARed Cross War Mem Childrens Hosp, Cape Town, South AfricaAin Shams Univ, Pediat Allergy Immunol & Rheumatol Unit, Cairo, EgyptAin Shams Univ, Pediat Rheumatol Pediat Allergy Immunol & Rheum, Cairo, EgyptKing Faisal Specialist Hosp & Res Ctr, Riyadh 11211, Saudi ArabiaCharles Univ Prague, Prague, Czech RepublicGen Univ Hosp, Prague, Czech RepublicUniv Hosp Motol, Dept Pediat, Prague, Czech RepublicAarhus Univ, Hosp Skejby, Aarhus, DenmarkRigshosp, Juliane Marie Ctr, DK-2100 Copenhagen, DenmarkUniv Med Ctr, Dept Pediat Immunol, Utrecht, NetherlandsWilhelmina Childrens Hosp, Utrecht, NetherlandsGreat Ormond St Hosp Sick Children, Children NHS Fdn Trust, Renal Unit, London, EnglandLyon Univ, Hosp Civils Lyon, Rheumatol & Dermatol Dept, Lyon, FranceMed Univ Innsbruck, A-6020 Innsbruck, AustriaPrim Univ Doz, Bregenz, AustriaHamburg Ctr Pediat & Adolescence Rheumatol, Hamburg, GermanyAsklepios Clin Sankt, Augustin, GermanyUniv Zurich, Childrens Hosp, Zurich, SwitzerlandAristotle Univ Thessaloniki, Pediat Immunol & Rheumatol Referral Ctr, GR-54006 Thessaloniki, GreeceIsrael Meir Hosp, Kefar Sava, IsraelSanjay Gandhi Postgrad Inst Med Sci, Lucknow, Uttar Pradesh, IndiaSemmelweis Univ, H-1085 Budapest, HungaryAnna Meyer Hosp, Florence, ItalyUniv Siena, Res Ctr System Autoimmune & Autoinflammatory Dis, I-53100 Siena, ItalyUniv Florence, Florence, ItalyOsped Pediat Bambino Gesu, IRCCS, Pediat Rheumatol Unit, Rome, ItalyUniv Genoa Pediat II Reumatol, Ist G Gaslini EULAR, Ctr Excellence Rheumatol, Genoa, ItalyUniv Cattolica Sacro Cuore, Inst Pediat, Rome, ItalyUniv Padua, Dept Pediat, Pediat Rheumatol Unit, Padua, ItalyYokohama City Univ, Sch Med, Yokohama, Kanagawa 232, JapanUniv Estadual Paulista, UNESP, Botucatu, SP, BrazilUniversidade Federal de São Paulo, Dept Pediat, São Paulo, BrazilUniv Estadual Campinas, Dept Med, Campinas, SP, BrazilUniv Fed Rio de Janeiro, Dept Pediat, Rio de Janeiro, BrazilUniv Estado do, Adolescent Hlth Care Unit, Div Pediat Rheumatol, Rio de Janeiro, BrazilUniv São Paulo, Fac Med, Childrens Inst, Dept Pediat,Pediat Rheumatol Unit, São Paulo, BrazilChildrens Inst, Pediat Rheumatol Unit, São Paulo, BrazilClin Pediat I, Cluj Napoca, RomaniaInst Rheumatol, Belgrade, SerbiaUniv Childrens Hosp, Univ Med Ctr Ljubljana, Ljubljana, SloveniaHead Rheumatol Hosp Pedro Elizalde, Buenos Aires, DF, ArgentinaHosp Gen Mexico City, Mexico City, DF, MexicoHosp Infantil Mexico Fed Gomez, Mexico City, DF, MexicoHosp San Juan Dios, Barcelona, SpainHosp Univ Valle Hebron, Barcelona, SpainMt Sinai Med Ctr, New York, NY 10029 USAMt Sinai Med Ctr, Miami Beach, FL 33140 USAComplejo Hosp Univ Ruiz & Paez, Bolivar, VenezuelaHacettepe Univ, Dept Pediat, Ankara, TurkeyIstanbul Univ, Cerrahpasa Med Sch, Istanbul, TurkeyFMF Arthrit Vasculitis & Orphan Dis Res Ctr, Inst Hlth Sci, Ankara, TurkeyUniv Calgary, Dept Pediat, Alberta Childrens Hosp, Res Inst, Calgary, AB T2N 1N4, CanadaUniversidade Federal de São Paulo, Dept Pediat, São Paulo, BrazilWeb of Scienc

Multidifferential study of identified charged hadron distributions in ZZ-tagged jets in proton-proton collisions at s=\sqrt{s}=13 TeV

Jet fragmentation functions are measured for the first time in proton-proton collisions for charged pions, kaons, and protons within jets recoiling against a $Z$ boson. The charged-hadron distributions are studied longitudinally and transversely to the jet direction for jets with transverse momentum 20 $< p_{\textrm{T}} < 100$ GeV and in the pseudorapidity range $2.5 < \eta < 4$. The data sample was collected with the LHCb experiment at a center-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 1.64 fb$^{-1}$. Triple differential distributions as a function of the hadron longitudinal momentum fraction, hadron transverse momentum, and jet transverse momentum are also measured for the first time. This helps constrain transverse-momentum-dependent fragmentation functions. Differences in the shapes and magnitudes of the measured distributions for the different hadron species provide insights into the hadronization process for jets predominantly initiated by light quarks.Comment: All figures and tables, along with machine-readable versions and any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-013.html (LHCb public pages

Study of the B−→Λc+Λˉc−K−B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-} decay

The decay $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ is studied in proton-proton collisions at a center-of-mass energy of $\sqrt{s}=13$ TeV using data corresponding to an integrated luminosity of 5 $\mathrm{fb}^{-1}$ collected by the LHCb experiment. In the $\Lambda_{c}^+ K^{-}$ system, the $\Xi_{c}(2930)^{0}$ state observed at the BaBar and Belle experiments is resolved into two narrower states, $\Xi_{c}(2923)^{0}$ and $\Xi_{c}(2939)^{0}$, whose masses and widths are measured to be $$m(\Xi_{c}(2923)^{0}) = 2924.5 \pm 0.4 \pm 1.1 \,\mathrm{MeV}, \\ m(\Xi_{c}(2939)^{0}) = 2938.5 \pm 0.9 \pm 2.3 \,\mathrm{MeV}, \\ \Gamma(\Xi_{c}(2923)^{0}) = \phantom{000}4.8 \pm 0.9 \pm 1.5 \,\mathrm{MeV},\\ \Gamma(\Xi_{c}(2939)^{0}) = \phantom{00}11.0 \pm 1.9 \pm 7.5 \,\mathrm{MeV},$$ where the first uncertainties are statistical and the second systematic. The results are consistent with a previous LHCb measurement using a prompt $\Lambda_{c}^{+} K^{-}$ sample. Evidence of a new $\Xi_{c}(2880)^{0}$ state is found with a local significance of $3.8\,\sigma$, whose mass and width are measured to be $2881.8 \pm 3.1 \pm 8.5\,\mathrm{MeV}$ and $12.4 \pm 5.3 \pm 5.8 \,\mathrm{MeV}$, respectively. In addition, evidence of a new decay mode $\Xi_{c}(2790)^{0} \to \Lambda_{c}^{+} K^{-}$ is found with a significance of $3.7\,\sigma$. The relative branching fraction of $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ with respect to the $B^{-} \to D^{+} D^{-} K^{-}$ decay is measured to be $2.36 \pm 0.11 \pm 0.22 \pm 0.25$, where the first uncertainty is statistical, the second systematic and the third originates from the branching fractions of charm hadron decays.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-028.html (LHCb public pages

Measurement of the ratios of branching fractions R(D∗)\mathcal{R}(D^{*}) and R(D0)\mathcal{R}(D^{0})

The ratios of branching fractions $\mathcal{R}(D^{*})\equiv\mathcal{B}(\bar{B}\to D^{*}\tau^{-}\bar{\nu}_{\tau})/\mathcal{B}(\bar{B}\to D^{*}\mu^{-}\bar{\nu}_{\mu})$ and $\mathcal{R}(D^{0})\equiv\mathcal{B}(B^{-}\to D^{0}\tau^{-}\bar{\nu}_{\tau})/\mathcal{B}(B^{-}\to D^{0}\mu^{-}\bar{\nu}_{\mu})$ are measured, assuming isospin symmetry, using a sample of proton-proton collision data corresponding to 3.0 fb${ }^{-1}$ of integrated luminosity recorded by the LHCb experiment during 2011 and 2012. The tau lepton is identified in the decay mode $\tau^{-}\to\mu^{-}\nu_{\tau}\bar{\nu}_{\mu}$. The measured values are $\mathcal{R}(D^{*})=0.281\pm0.018\pm0.024$ and $\mathcal{R}(D^{0})=0.441\pm0.060\pm0.066$, where the first uncertainty is statistical and the second is systematic. The correlation between these measurements is $\rho=-0.43$. Results are consistent with the current average of these quantities and are at a combined 1.9 standard deviations from the predictions based on lepton flavor universality in the Standard Model.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-039.html (LHCb public pages

Inquérito epidemiológico de doenças respiratórias em aves de subsistência e modelagem de espalhamento de influenza aviária no Rio Grande do Sul

Patógenos associados a aves migratórias podem causar doenças em aves domésticas. As aves de subsistência são possíveis fontes de disseminação destes patógenos pela ausência de biossegurança na criação e pelo contato com aves silvestres. A região do Parque Nacional da Lagoa do Peixe (PNLP), no Rio Grande do Sul, se caracteriza como um importante sítio de aves migratórias. Devido a isto, é possível uma interface entre aves migratórias e aves de subsistência criadas na região. A influenza aviária (AI) é a principal doença associada a aves migratórias que afeta o sistema respiratório, sendo estas os principais disseminadores do vírus. Estudos prévios indicam que há risco de introdução de um vírus de AI de alta patogenicidade (HPAIV) na região. Porém, o padrão e impacto do espalhamento deste patógeno na região é desconhecido, visto a ausência de relatos. Cabe ressaltar que outras enfermidades respiratórias, como a Doença de Newcasle (ND) e a micoplasmose por Mycoplasma gallisepticum (MG), também são associadas a aves migratórias e podem causar doenças em aves domésticas. Neste contexto, o objetivo deste trabalho é realizar um inquérito epidemiológico de doenças respiratórias associadas a aves migratórias em aves de subsistência e propor um modelo de espalhamento de HPAIV na região do PNLP. Com esta finalidade, realizamos uma avaliação (i) da presença de anticorpos contra AI, ND e MG nas aves de subsistência por ELISA, (ii) da presença dos agentes de AI e ND por RT-PCR em tempo real nas aves de subsistência, (iii) dos possíveis fatores de risco associados aos patógenos e (iv) das consequências da introdução de HPAIV na região através de um modelo matemático de espalhamento. Nas propriedades de aves de subsistência amostradas da região, foi detectada a presença de anticorpos contra AI, ND e MG na frequência de 4,2%, 87,5% e 58,3%, respectivamente. Todas as amostras foram negativas no RT-PCR em tempo real realizado para AI e ND. A avaliação de fatores de risco foi possível nas propriedades soropositivas para ND e MG, possibilitando realizar-se a primeira análise de fatores de risco para estes agentes em aves de subsistência no Brasil. Para ND, o risco foi maior nas propriedades nas quais os criadores adotam a prática de reposição própria para manter a criação (PR=1,64; 95% IC: 1,11 – 2,42). Além disso, o aumento na distância das granjas em relação ao estuário da Laguna do Peixe diminui o risco para ND (PR=0,94; 95% IC: 0,90 – 0,99). Já para MG, foram considerados fatores de risco a prática de confinar as aves (PR = 3,40, 95% IC: 1,93 – 5,99) e a interação entre a “troca de aves ou ovos com outros produtores e vizinhos possuírem aves” (PR = 2,16, 95% IC: 1,24 – 3,76). Nas simulações da modelagem matemática de espalhamento de HPAIV na região, a maioria das propriedades se tornaria infectada até o 30º dia de infecção. Além disso, na média das simulações, a infecção atingiria em torno de 80 a 90% das propriedades, alcançando até 30 km de distância do caso índice. Os resultados indicam a circulação de patógenos associados a aves migratórias na região do PNLP. Devido à detecção destes nas aves de subsistência, estas podem ser consideradas sentinelas destes agentes na região. Além disso, os dados obtidos incrementam o conhecimento sobre o espalhamento e a dinâmica dessas doenças em propriedades com aves de subsistência, bem como sobre os indicadores de risco para ocorrência. Estes dados podem ser utilizados para o estabelecimento de medidas de biossegurança e de manejo adequados para este tipo de criação e de programas de monitoria visando à contenção da disseminação de patógenos.Pathogens associated with migratory birds can cause disease in domestic birds. Backyard poultry are possible source of pathogens dissemination due to the lack of biosecurity measures and the contact with wild birds. The region of Lagoa do Peixe National Park (PNLP), in Rio Grande do Sul state, Brazil, is characterized as an important site for migratory birds. Thus, it is a possible interface place for migratory and backyard birds. Avian influenza (AI) is the most important respiratory disease associated with migratory birds, the main virus disseminating agents. Previous studies have indicated a risk for introduction of higly pathogenic avian influenza virus (HPAIV) in PNLP region. However, the impact and spreading pattern of AI virus in this region is unknown due to the lack of disease reports. It is noteworthy that other respiratory diseases, such Newcastle Disease (ND) and micoplasmosis due to Mycoplasma gallisepticum (MG), are also associated with migratory birds and can cause disturbances in domestic poultry. In this context, the aims of this work are to perform an epidemiological survey of respiratory diseases associated with migratory birds in backyard poultry and to propose a model of HPAIV spreading in PNLP region. For this, it was evaluated (i) the presence of antibodies against AI, ND and MG in backyard poultry by ELISA, (ii) the presence of AI and ND virus in backyard poultry by real time RT-PCR, (iii) the potential risk factors associated with these pathogens, and (iv) the consequences of HPAIV introduction in PNLP region using a mathematical model of virus spreading. In the sampled properties, frequency of antibodies against AI, ND and MG were 4.2%, 87.5% and 58.3%, respectively. All samples were negative for AI and ND in real time RT-PCR analysis. The first evaluation of risk factors associated to ND or MG in backyard poultry from Brazil was performed in this work. For ND, the risk was increased in the properties in which farmers used their own replacement poultry to restock their flock (PR=1.64; 95% CI: 1.11–2.42). Furthermore, the increasing distance of the household flock to the “Laguna do Peixe” estuary is associated with decreasing NDV seropositivity (PR=0.94; 95% CI: 0.90 – 0.99). For MG, seropositivity was significantly associated with bird confinement (PR = 3.40, 95% CI: 1.93 – 5.99) and with interaction between “poultry/egg exchange and neighbors have poultry” (PR = 2.16, 95% CI: 1.24 – 3.76). In the simulations from the mathematical model of HPAIV spreading in PNLP region, most of the properties of this region would become infected up to 30 days outbreak beginning. Moreover, the infection would affect about 80 to 90% of the properties from the region, reaching up to 30 km distance from the index case. The results showed the circulation of pathogens associated with migratory birds in PNLP region. Since these pathogens were detected in backyard poultry, these birds can be considered sentinels for these agents in this region. Additionally, the data obtained improve the knowledge on spreading and dynamics of these diseases in properties with backyard poultry, as well as on potential risk factors. The set of these results can be useful to development of adequate biosecurity procedures for backyard flocks and surveillance programs to avoid pathogen dissemination