Examining the impact of agricultural drainage ditch management on in-stream bacterial communities involved in nitrogen cycling: insights from the Environmental Change One Health Observatory (ECO2)

IntroductionAgricultural drainage ditches are essential for maintaining flow efficiency and are often managed by brushing (i.e., vegetation clearing along ditch banks) and dredging. These maintenance practices not only impact flow but also the ditches’ capacity to process and assimilate agricultural chemicals. However, the influence of ditch management on microbial activities, essential in processing and transforming these contaminants, remains underexplored.MethodsWe conducted a four-year surface water sampling campaign in two long-established (over 40 years) agricultural drainage ditches situated in eastern Ontario, Canada. One drainage ditch underwent intensive bank brushing and channel dredging, while the other remained unmanaged. We assessed the impact of these contrasting ditch management practices on the diversity and compositional structure of surface water microbiome communities, with a particular focus on the distribution of genes associated with nitrogen (N) cycling, using shotgun metagenomics sequencing and an assembly-based metagenome approach.Results and discussionFrom 117 surface water samples, we reconstructed 157 metagenome-assembled genomes (MAGs), predominantly from the Patescibacteria superphylum. Brushing alone, conducted about 10 months prior to dredging, had an insignificant impact on the aquatic microbial community. However, dredging led to a significant reduction in community diversity and abundance of genes affiliated with denitrification, dissimilatory nitrate reduction to ammonia, and complete nitrification through comammox. Ditch management resulted in noticeable shifts in the microbial community, evidenced by the enrichment of taxa in Polaromonas, Emticicia, Flectobacillus, and Patescibacteria in the water of the managed ditch sites. The metagenomes of these taxa harbor genes involved in various nitrogen biotransformation pathways. Interestingly, following dredging, the levels of ammonia and ammonium, nitrite, and total Kjeldahl N in the managed ditch did not increase, but significantly decreased; likely due to ditch substrate removal and potentially increased flow efficiency and dilution. Being one of the few studies conducted to date, this study provided unique insights into the consequences of drainage ditch management on freshwater microbiomes involved in N cycling

Japanese cross-cultural validation study of the Pain Stage of Change Questionnaire.

Introduction:Although evidence supports efficacy of treatments that enhance self-management of chronic pain, the efficacy of these treatments has been hypothesized to be influenced by patient readiness for self-management. The Pain Stage of Change Questionnaire (PSOCQ) is a reliable and valid measure of patient readiness to self-manage pain. However, there is not yet a Japanese version of the PSOCQ (PSOCQ-J), which limits our ability to evaluate the role of readiness for pain self-management in function and treatment response in Japanese patients with chronic pain.Objective:Here, we sought to develop the PSOCQ-J and evaluate its psychometric properties.Methods:We recruited 201 patients with chronic pain. The study participants were asked to complete the PSOCQ-J and other measures assessing pain severity, pain interference, catastrophizing, self-efficacy, and pain coping strategies.Results:The results supported a 4-factor structure of the PSOCQ-J. We also found good to excellent internal consistencies and good test-retest reliabilities for the 4 scales. The Precontemplation scale had weak to moderate positive correlations with measures of pain-related dysfunction and maladaptive coping. The Action and Maintenance scales had weak to moderate positive correlations with measures of self-efficacy and adaptive coping. The Contemplation scale had weak positive correlations with measures of pain interference and both adaptive and maladaptive coping.Conclusions:The PSOCQ-J demonstrated adequate psychometric properties in a sample of Japanese patients with chronic pain. This measure can be used to evaluate the role that readiness to self-manage pain may play in adjustment to chronic pain in Japanese pain populations

Using Artificial Neural Networks and Remotely Sensed Data to Evaluate the Relative Importance of Variables for Prediction of Within-Field Corn and Soybean Yields

Crop yield prediction prior to harvest is important for crop income and insurance projections, and for evaluating food security. Yet, modeling crop yield is challenging because of the complexity of the relationships between crop growth and predictor variables, especially at the field scale. In this study, an artificial neural network (ANN) method was used: (1) to evaluate the relative importance of predictor variables for the prediction of within-field corn and soybean end-of-season yield and (2) to evaluate the performance of the ANN models with a minimal optimized variable dataset for their capacity to predict corn and soybean yield over multiple years at the within-field level. Several satellite derived vegetation indices (normalized difference vegetation index—NDVI, red edge NDVI and simple ratio—SR) and elevation derived variables (slope, flow accumulation, aspect) were used as crop yield predictor variables, hypothesizing that the different variables reflect different crop and site conditions. The study identified the SR index and the slope as the most important predictor variables for both crop types during two training and testing years (2011, 2012). The dates of the most important SR images, however, were different for the two crop types and corresponded to their critical crop developmental stages (phenology). The relative mean absolute errors were overall smaller for corn compared to soybean: all of the 2011 corn study fields had errors below 10%; 75% of the fields had errors below 10% in 2012. The errors were more variable for soybean. In 2011, 37% of the fields had errors below 10%, while in 2012, 100% of the fields had errors below 20%. The results are promising and can provide yield estimates at the farm level, which could be useful in refining broader scale (e.g., county, region) yield projections

Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Abstract Background Arcobacter faecis and A. lanthieri are two newly classified species of genus Arcobacter. The prevalence and distribution of virulence, antibiotic resistance and toxin (VAT) genes in these species are required to assess their potential pathogenic health impacts to humans and animals. This study (i) developed species- and gene-specific primer pairs for the detection of six virulence, two antibiotic resistance, and three toxin genes in two target species; (ii) optimized eight single-tube multiplex and three monoplex PCR protocols using the newly developed species- and gene-specific primers; and (iii) conducted specificity and sensitivity evaluations as well as validation of eleven mono- and multiplex PCR assays by testing A. faecis (n= 29) and A. lanthieri (n= 10) strains isolated from various fecal and agricultural water sources to determine the prevalence and distribution of VAT genes and assess the degree of pathogenicity within the two species. Results Detection of all ten and eleven target VAT genes, and expression of cytolethal distending toxin (cdtA, cdtB and cdtC) genes in A. faecis and A. lanthieri reference strains with high frequency in field isolates suggest that they are potentially pathogenic strains. These findings indicate that these two species can pose a health risk to humans and animals. Conclusions The study results show that the developed mono- and multiplex PCR (mPCR) assays are simple, rapid, reliable and sensitive for the simultaneous assessment of the potential pathogenicity and antibiotic resistance profiling of tet(O) and tet(W) genes in these two newly discovered species. Also, these assays can be useful in diagnostic and analytical laboratories to determine the pathotypes and assessment of the virulence and toxin factors associated to human and animal infections

Japanese cross-cultural validation study of the Pain Stage of Change Questionnaire

Abstract. Introduction:. Although evidence supports efficacy of treatments that enhance self-management of chronic pain, the efficacy of these treatments has been hypothesized to be influenced by patient readiness for self-management. The Pain Stage of Change Questionnaire (PSOCQ) is a reliable and valid measure of patient readiness to self-manage pain. However, there is not yet a Japanese version of the PSOCQ (PSOCQ-J), which limits our ability to evaluate the role of readiness for pain self-management in function and treatment response in Japanese patients with chronic pain. Objective:. Here, we sought to develop the PSOCQ-J and evaluate its psychometric properties. Methods:. We recruited 201 patients with chronic pain. The study participants were asked to complete the PSOCQ-J and other measures assessing pain severity, pain interference, catastrophizing, self-efficacy, and pain coping strategies. Results:. The results supported a 4-factor structure of the PSOCQ-J. We also found good to excellent internal consistencies and good test–retest reliabilities for the 4 scales. The Precontemplation scale had weak to moderate positive correlations with measures of pain-related dysfunction and maladaptive coping. The Action and Maintenance scales had weak to moderate positive correlations with measures of self-efficacy and adaptive coping. The Contemplation scale had weak positive correlations with measures of pain interference and both adaptive and maladaptive coping. Conclusions:. The PSOCQ-J demonstrated adequate psychometric properties in a sample of Japanese patients with chronic pain. This measure can be used to evaluate the role that readiness to self-manage pain may play in adjustment to chronic pain in Japanese pain populations

The slow death of Japanese techno-nationalism? Emerging comparative lessons for China's defense production

Japan's defense production model is often portrayed as an exemplar of techno-nationalism, but can it serve as a model for China to follow in pursuit of technological military catch-up? Japan in the past has exploited civilian industrial strengths to create a defense production base with footholds in key technologies. However, Japan's defense production model is now displaying structural limits-constrained defense budgets, deficient procurement management, limited international collaboration-with the risks of civilian industry exiting the sector, the loss of even basic competency in military technologies, and the consequent weakening of national security autonomy. Japan's case thus offers emerging comparative lessons for China to study in what to do and not to in pursuing civilian-military integration