A comparison of direct versus self-report measures for assessing physical activity in adults: a systematic review

<p>Abstract</p> <p>Background</p> <p>Accurate assessment is required to assess current and changing physical activity levels, and to evaluate the effectiveness of interventions designed to increase activity levels. This study systematically reviewed the literature to determine the extent of agreement between subjectively (self-report e.g. questionnaire, diary) and objectively (directly measured; e.g. accelerometry, doubly labeled water) assessed physical activity in adults.</p> <p>Methods</p> <p>Eight electronic databases were searched to identify observational and experimental studies of adult populations. Searching identified 4,463 potential articles. Initial screening found that 293 examined the relationship between self-reported and directly measured physical activity and met the eligibility criteria. Data abstraction was completed for 187 articles, which described comparable data and/or comparisons, while 76 articles lacked comparable data or comparisons, and a further 30 did not meet the review's eligibility requirements. A risk of bias assessment was conducted for all articles from which data was abstracted.</p> <p>Results</p> <p>Correlations between self-report and direct measures were generally low-to-moderate and ranged from -0.71 to 0.96. No clear pattern emerged for the mean differences between self-report and direct measures of physical activity. Trends differed by measure of physical activity employed, level of physical activity measured, and the gender of participants. Results of the risk of bias assessment indicated that 38% of the studies had lower quality scores.</p> <p>Conclusion</p> <p>The findings suggest that the measurement method may have a significant impact on the observed levels of physical activity. Self-report measures of physical activity were both higher and lower than directly measured levels of physical activity, which poses a problem for both reliance on self-report measures and for attempts to correct for self-report – direct measure differences. This review reveals the need for valid, accurate and reliable measures of physical activity in evaluating current and changing physical activity levels, physical activity interventions, and the relationships between physical activity and health outcomes.</p

Connecting young star clusters to CO molecular gas in NGC 7793 with ALMA-LEGUS

We present an investigation of the relationship between giant molecular cloud (GMC) properties and the associated stellar clusters in the nearby flocculent galaxy NGC 7793. We combine the star cluster catalogue from the HST LEGUS (Legacy ExtraGalactic UV Survey) programme with the 15 pc resolution ALMA CO(2–1) observations. We find a strong spatial correlation between young star clusters and GMCs such that all clusters still associated with a GMC are younger than 11 Myr and display a median age of 2 Myr. The age distribution increases gradually as the cluster–GMC distance increases, with star clusters that are spatially unassociated with molecular gas exhibiting a median age of 7 Myr. Thus, star clusters are able to emerge from their natal clouds long before the time-scale required for clouds to disperse. To investigate if the hierarchy observed in the stellar components is inherited from the GMCs, we quantify the amount of clustering in the spatial distributions of the components and find that the star clusters have a fractal dimension slope of −0.35 ± 0.03, significantly more clustered than the molecular cloud hierarchy with slope of −0.18 ± 0.04 over the range 40–800 pc. We find, however, that the spatial clustering becomes comparable in strength for GMCs and star clusters with slopes of −0.44 ± 0.03 and −0.45 ± 0.06, respectively, when we compare massive (>105 M⊙) GMCs to massive and young star clusters. This shows that massive star clusters trace the same hierarchy as their parent GMCs, under the assumption that the star formation efficiency is a few per cent.Support for Program 13364 was provided by NASA through a grant from the Space Telescope Science Institute. This research has made use of the NASA/IPAC Extragalactic Database (NED) which is operated by the Jet Propulsion Laboratory, California Institute of Technology, under contract with NASA. This paper makes use of the following ALMA data: ADS/JAO.ALMA #2015.1.00782.S. ALMA is a partnership of ESO (representing its member states), NSF (USA) and NINS (Japan), together with NRC (Canada) and NSC and ASIAA (Taiwan) and KASI (Republic of Korea), in cooperation with the Republic of Chile. The Joint ALMA Observatory is operated by ESO, AUI/NRAO, and NAOJ. The National Radio Astronomy Observatory is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc. Parts of this research were supported by the Australian Research Council Centre of Excellence for All Sky Astrophysics in three Dimensions (ASTRO 3D), through project number CE170100013. AA acknowledges the support of the Swedish Research Council (Vetenskapsradet) and the Swedish National Space Board (SNSB). ˚ MF acknowledges support by the Science and Technology Facilities Council [grant number ST/P000541/1]. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement number 757535

Dust Buried Compact Sources in the Dwarf Galaxy NGC 4449

Multi-wavelength images from the Hubble Space Telescope covering the wavelength range 0.27-1.6 $\mu$m show that the central area of the nearby dwarf galaxy NGC4449 contains several tens of compact sources that are emitting in the hydrogen recombination line Pa$\beta$ (1.2818 $\mu$m) but are only marginally detected in H$\alpha$ (0.6563 $\mu$m) and undetected at wavelengths $\lambda\le$0.55 $\mu$m. An analysis of the spectral energy distributions (SEDs) of these sources indicates that they are likely relatively young star clusters heavily attenuated by dust. The selection function used to identify the sources prevents meaningful statistical analyses of their age, mass, and dust extinction distributions. However, these cluster candidates have ages $\sim$5-6 Myr and A$_V>$6 mag, according to their SED fits, and are extremely compact, with typical deconvolved radii of 1 pc. The dusty clusters are located at the periphery of dark clouds within the galaxy and appear to be partially embedded. Density and pressure considerations indicate that the HII regions surrounding these clusters may be stalled, and that pre-supernova feedback has not been able to clear the clusters of their natal cocoons. These findings are in potential tension with existing models that regulate star formation with pre-supernova feedback, since pre-supernova feedback acts on short timescales, $\lesssim$4 Myr, for a standard Stellar Initial Mass function. The existence of a population of dusty star clusters with ages $>$4 Myr, if confirmed by future observations, paints a more complex picture for the role of stellar feedback in controlling star formation.Comment: 27 pages, 22 figures; accepted for publication in the Astrophysical Journa

Biophysical and Functional Characterization of Rhesus Macaque IgG Subclasses

Antibodies raised in Indian rhesus macaques [Macaca mulatta (MM)] in many preclinical vaccine studies are often evaluated in vitro for titer, antigen-recognition breadth, neu- tralization potency, and/or effector function, and in vivo for potential associations with protection. However, despite reliance on this key animal model in translation of promising candidate vaccines for evaluation in first in man studies, little is known about the proper- ties of MM immunoglobulin G (IgG) subclasses and how they may compare to human IgG subclasses. Here, we evaluate the binding of MM IgG1, IgG2, IgG3, and IgG4 to human Fc gamma receptors (FcγR) and their ability to elicit the effector functions of human FcγR-bearing cells, and unlike in humans, find a notable absence of subclasses with dramatically silent Fc regions. Biophysical, in vitro, and in vivo characterization revealed MM IgG1 exhibited the greatest effector function activity followed by IgG2 and then IgG3/4. These findings in rhesus are in contrast with the canonical understanding that IgG1 and IgG3 dominate effector function in humans, indicating that subclass-switching profiles observed in rhesus studies may not strictly recapitulate those observed in human vaccine studies

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.

Age at menarche is a marker of timing of puberty in females. It varies widely between individuals, is a heritable trait and is associated with risks for obesity, type 2 diabetes, cardiovascular disease, breast cancer and all-cause mortality. Studies of rare human disorders of puberty and animal models point to a complex hypothalamic-pituitary-hormonal regulation, but the mechanisms that determine pubertal timing and underlie its links to disease risk remain unclear. Here, using genome-wide and custom-genotyping arrays in up to 182,416 women of European descent from 57 studies, we found robust evidence (P < 5 × 10(-8)) for 123 signals at 106 genomic loci associated with age at menarche. Many loci were associated with other pubertal traits in both sexes, and there was substantial overlap with genes implicated in body mass index and various diseases, including rare disorders of puberty. Menarche signals were enriched in imprinted regions, with three loci (DLK1-WDR25, MKRN3-MAGEL2 and KCNK9) demonstrating parent-of-origin-specific associations concordant with known parental expression patterns. Pathway analyses implicated nuclear hormone receptors, particularly retinoic acid and γ-aminobutyric acid-B2 receptor signalling, among novel mechanisms that regulate pubertal timing in humans. Our findings suggest a genetic architecture involving at least hundreds of common variants in the coordinated timing of the pubertal transition

Winning Fights Induces Hyperaggression via the Action of the Biogenic Amine Octopamine in Crickets

Winning an agonistic interaction against a conspecific is known to heighten aggressiveness, but the underlying events and mechanism are poorly understood. We quantified the effect of experiencing successive wins on aggression in adult male crickets (Gryllus bimaculatus) by staging knockout tournaments and investigated its dependence on biogenic amines by treatment with amine receptor antagonists. For an inter-fight interval of 5 min, fights between winners escalated to higher levels of aggression and lasted significantly longer than the preceding round. This winner effect is transient, and no longer evident for an inter-fight interval of 20 min, indicating that it does not result from selecting individuals that were hyper-aggressive from the outset. A winner effect was also evident in crickets that experienced wins without physical exertion, or that engaged in fights that were interrupted before a win was experienced. Finally, the winner effect was abolished by prior treatment with epinastine, a highly selective octopamine receptor blocker, but not by propranolol, a ß-adrenergic receptor antagonist, nor by yohimbine, an insect tyramine receptor blocker nor by fluphenazine an insect dopamine-receptor blocker. Taken together our study in the cricket indicates that the physical exertion of fighting, together with some rewarding aspect of the actual winning experience, leads to a transient increase in aggressive motivation via activation of the octopaminergic system, the invertebrate equivalent to the adrenergic system of vertebrates

Genetic association study of QT interval highlights role for calcium signaling pathways in myocardial repolarization.

The QT interval, an electrocardiographic measure reflecting myocardial repolarization, is a heritable trait. QT prolongation is a risk factor for ventricular arrhythmias and sudden cardiac death (SCD) and could indicate the presence of the potentially lethal mendelian long-QT syndrome (LQTS). Using a genome-wide association and replication study in up to 100,000 individuals, we identified 35 common variant loci associated with QT interval that collectively explain ∼8-10% of QT-interval variation and highlight the importance of calcium regulation in myocardial repolarization. Rare variant analysis of 6 new QT interval-associated loci in 298 unrelated probands with LQTS identified coding variants not found in controls but of uncertain causality and therefore requiring validation. Several newly identified loci encode proteins that physically interact with other recognized repolarization proteins. Our integration of common variant association, expression and orthogonal protein-protein interaction screens provides new insights into cardiac electrophysiology and identifies new candidate genes for ventricular arrhythmias, LQTS and SCD

Incidence of Type 2 Diabetes in Children With Nonalcoholic Fatty Liver Disease

Background &amp; aimsType 2 diabetes (T2D) is a growing problem in children. Children with NAFLD are at potentially high risk for developing T2D; however, the incidence of T2D in this population is unknown. This study aimed to determine the incidence of T2D in children with NAFLD and identify associated risk factors.MethodsChildren with NAFLD enrolled in the Nonalcoholic Steatohepatitis Clinical Research Network were followed longitudinally. Incidence of T2D was determined by using clinical history and fasting laboratory values. Cumulative incidence curves were developed for time to T2D. A Cox regression multivariable model was constructed using best subsets Akaike's Information Criteria selection.ResultsThis study included 892 children with NAFLD and with a mean age of 12.8 years (2.7) followed for 3.8 years (2.3) with a total 3234 person-years at risk. The incidence rate of T2D was 3000 new cases per 100,000 person-years at risk. At baseline, 63 children had T2D, and during follow-up, an additional 97 children developed incident T2D, resulting in a period prevalence of 16.8%. Incident T2D was significantly higher in females versus males (hazard ratio [HR], 1.8 [1.0-2.8]), associated with BMI z-score (HR, 1.8 [1.0-3.0]), and more severe liver histology including steatosis grade (HR, 1.3 [1.0-1.7]), and fibrosis stage (HR, 1.3 [1.0-1.5]).ConclusionsChildren with NAFLD are at high risk for existing and incident T2D. In addition to known risk factors for T2D (female and BMI z-score), severity of liver histology at the time of NAFLD diagnosis was independently associated with T2D development. Targeted strategies to prevent T2D in children with NAFLD are needed

Feedback in Emerging Extragalactic Star Clusters, FEAST: The relation between 3.3 μ m polycyclic aromatic hydrocarbon emission and star formation rate traced by ionized gas in NGC 628

We present maps of ionized gas (traced by Paα and Brα) and 3.3 μm polycyclic aromatic hydrocarbon (PAH) emission in the nearby spiral galaxy NGC 628, derived from new JWST/NIRCam data from the Feedback in Emerging extrAgalactic Star clusTers (FEAST) survey. With this data, we investigate and calibrate the relation between 3.3 μm PAH emission and star formation rate (SFR) in and around emerging young star clusters (eYSCs) on a scale of ∼40 pc. We find a tight (correlation coefficient ρ ∼ 0.9) sublinear (power-law exponent α ∼ 0.75) relation between the 3.3 μm PAH luminosity surface density and SFR traced by Brα for compact, cospatial (within 0.″16 or ∼7 pc) peaks in Paα, Brα, and 3.3 μm (eYSC–I). The scatter in the relationship does not correlate well with variations in local interstellar medium metallicity, due to a radial metallicity gradient, but rather is likely due to stochastic sampling of the stellar initial mass function (IMF) and variations in the PAH heating and age of our sources. The deviation from a linear relation may be explained by PAH destruction in more intense ionizing environments, variations in age, and IMF stochasticity at intermediate to low luminosities. We test our results with various continuum subtraction techniques using combinations of NIRCam bands and find that they remain robust with only minor differences in the derived slope and intercept. An unexpected discrepancy is identified between the relations of hydrogen recombination lines (Paα versus Brα; Hα versus Brα)