Detection of Hemlock Woolly Adelgid (Hemiptera: Adelgidae) Infestations with Sticky Traps

We deployed sticky traps underneath the crown of eastern hemlock, Tsuga canadensis (L.) Carrière, to assess their sensitivity at detecting crawlers (1st instar nymphs) of the non-native hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae). We found these traps more sensitive at detecting infested trees with low densities of A. tsugae than branch-tip sampling with pole pruners. We observed two peaks of crawler abundance at all sites: these peaks likely represented the timing of the progrediens and sistens crawler stages of A. tsugae. Deployment of sticky traps in treated and high-risk stands may prove useful at detecting residual and new infestations, respectively

Mannose binding lectin is required for alphavirus-induced arthritis/myositis

Mosquito-borne alphaviruses such as chikungunya virus and Ross River virus (RRV) are emerging pathogens capable of causing large-scale epidemics of virus-induced arthritis and myositis. The pathology of RRV-induced disease in both humans and mice is associated with induction of the host inflammatory response within the muscle and joints, and prior studies have demonstrated that the host complement system contributes to development of disease. In this study, we have used a mouse model of RRV-induced disease to identify and characterize which complement activation pathways mediate disease progression after infection, and we have identified the mannose binding lectin (MBL) pathway, but not the classical or alternative complement activation pathways, as essential for development of RRV-induced disease. MBL deposition was enhanced in RRV infected muscle tissue from wild type mice and RRV infected MBL deficient mice exhibited reduced disease, tissue damage, and complement deposition compared to wild-type mice. In contrast, mice deficient for key components of the classical or alternative complement activation pathways still developed severe RRV-induced disease. Further characterization of MBL deficient mice demonstrated that similar to C3(-/-) mice, viral replication and inflammatory cell recruitment were equivalent to wild type animals, suggesting that RRV-mediated induction of complement dependent immune pathology is largely MBL dependent. Consistent with these findings, human patients diagnosed with RRV disease had elevated serum MBL levels compared to healthy controls, and MBL levels in the serum and synovial fluid correlated with severity of disease. These findings demonstrate a role for MBL in promoting RRV-induced disease in both mice and humans and suggest that the MBL pathway of complement activation may be an effective target for therapeutic intervention for humans suffering from RRV-induced arthritis and myositis.This work was supported by NIH/NIAMS R01 AR 047190 awarded to MTH

Probing small-x parton densities in proton- proton (-nucleus) collisions in the very forward direction

We present calculations of several pp scattering cross sections with potential applications at the LHC. Significantly large rates for momentum fraction, x, as low as 10^-7 are obtained, allowing for possible extraction of quark and gluon densities in the proton and nuclei down to these small x values provided a detector with good acceptance at maximal rapidities is used.Comment: 14 pages, LaTeX, 12 figures, uses revtex.st

Ross River virus envelope glycans contribute to disease through activation of the host complement system

Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL. Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans. Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle. Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues. These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis

An Initial Mass Function Study of the Dwarf Starburst Galaxy NGC 4214

The production rate of ionizing photons in young (≤8 Myr), unresolved stellar clusters in the nearby irregular galaxy NGC 4214 is probed using multi-wavelength Hubble Space Telescope WFC3 data. We normalize the ionizing photon rate by the cluster mass to investigate the upper end of the stellar initial mass function (IMF). We have found that within the uncertainties the upper end of the stellar IMF appears to be universal in this galaxy, and that deviations from a universal IMF can be attributed to stochastic sampling of stars in clusters with masses ≾ 10^3 M_☉. Furthermore, we have found that there does not seem to be a dependence of the maximum stellar mass on the cluster mass. We have also found that for massive clusters, feedback may cause an underrepresentation in Hα luminosities, which needs to be taken into account when conducting this type of analysis