Reclassification of <i>Saccharomycodes sinensis</i>, Proposal of <i>Yueomyces sinensis</i> gen. nov., comb. nov. within Saccharomycetaceae (Saccharomycetales, Saccharomycotina)

<div><p>The phylogenetic position of <i>Saccharomycodes sinensis</i> has been debated by yeast taxonomists. In this study, a multigene phylogenetic analysis based on four regions, namely the 18S ribosomal DNA (rDNA), the D1/D2 domains of the 26S rDNA, the second largest subunit of RNA polymerase II gene (<i>RPB2</i>) and translation elongation factor 1-α gene (<i>EF1-α</i>), were performed to address the phylogenetic placement of <i>S</i>. <i>sinensis</i>. Our result indicated that <i>S</i>. <i>sinensis</i> belongs to Saccharomycetaceae instead of Saccharomycodaceae, and forms a single species lineage divergent from the other genera within Saccharomycetaceae. <i>Yueomyces</i> gen. nov. (MycoBank No. MB 811648) is proposed in the Saccharomycetaceae with <i>Y</i>. <i>sinensis</i> comb. nov. (MycoBank No. MB 811649, type strain CGMCC 2.01395^T = IFO 10111^T = CBS 7075^T) as the type species.</p></div

The multigene ML tree.

<p>Phylogenetic tree constructed from Maximum likelihood (ML) analysis of the combined sequences of the18S rDNA, D1/D2 domains of the 26S rDNA, the <i>RPB2</i> and <i>EF1-α</i>, depicting <i>Yueomyces sinensis</i> and other taxa in the Saccharomycetales (Saccharomycotina). Bootstrap percentages greater than 50% from 1000 bootstrap replicates are shown. Bar = 0.1 indicates 10% sequence divergence.</p

Distribution of MICs according to the phylum of clinical isolates and reference strains.

<p>Distribution of MICs according to the phylum of clinical isolates and reference strains.</p

Proportion of reference strains with high MIC.

*<p>Minimal inhibitory concentration.</p

Reference strains able to grow at 37°C with high MIC according to source of isolation.

<p>Reference strains able to grow at 37°C with high MIC according to source of isolation.</p