Diversity of the vegetation cover of the zone of potential influence of the Nizhneboguchanskaya HPP (Lower Angara region)

Active economic development of the Lower Angara zone requires the creation of a scientific basis for long-term monitoring of the state of natural ecosystems. The paper gives an assessment of the diversity of vegetation cover in the zone of potential influence of the Nizhneboguchanskaya HPP. An analysis of its typological structure on a landscape-ecological basis is given. The results of the DCA ordination and the interpretation of the leading axes of variation demonstrated the presence of four distinct groups of forest types that form ecological series according to the leading factors - the richness and hydrothermal regimes of soils. The dendrogram of forest types in the study area reflects the floristic integrity of the identified groups of forest types and the floristic relationships between them

Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Multiple studies have demonstrated that various nanoparticles (NPs) stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) and inhibit adipogenic ones. The mechanisms of these effects are not determined. The aim of this paper was to estimate Wharton’s Jelly MSCs phenotype and humoral factor production during tri-lineage differentiation per se and in the presence of silicon–gold NPs. Silicon (SiNPs), gold (AuNPs), and 10% Au-doped Si nanoparticles (SiAuNPs) were synthesized by laser ablation, characterized, and studied in MSC cultures before and during differentiation. Humoral factor production (n = 41) was analyzed by Luminex technology. NPs were nontoxic, did not induce ROS production, and stimulated G-CSF, GM-CSF, VEGF, CXCL1 (GRO) production in four day MSC cultures. During MSC differentiation, all NPs stimulated CD13 and CD90 expression in osteogenic cultures. MSC differentiation resulted in a decrease in multiple humoral factor production to day 14 of incubation. NPs did not significantly affect the production in chondrogenic cultures and stimulated it in both osteogenic and adipogenic ones. The major difference in the protein production between osteogenic and adipogenic MSC cultures in the presence of NPs was VEGF level, which was unaffected in osteogenic cells and 4–9 times increased in adipogenic ones. The effects of NPs decreased in a row AuNPs > SiAuNPs > SiNPs. Taken collectively, high expression of CD13 and CD90 by MSCs and critical level of VEGF production can, at least, partially explain the stimulatory effect of NPs on MSC osteogenic differentiation