In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome

We analyzed the genetic evolution of bovine respiratory syncytial virus (BRSV) isolate W2-00131, from its isolation in bovine turbinate (BT) cells to its inoculation in calves. Results showed that the BRSV genomic region encoding the highly variable glycoprotein G remains genetically stable after virus isolation and over 10 serial infections in BT cells, as well as following experimental inoculation in calves. This remarkable genetic stability led us to examine the mutant spectrum of several populations derived from this field isolate. Sequence analysis of molecular clones revealed an important genetic heterogeneity in G coding region of each population, with mutation frequencies ranging from 6.8 to 10.1 10-4 substitutions/nucleotide. The non-synonymous mutations of the mutant spectrum mapped preferentially within the two variable antigenic regions of the ectodomain or close to the highly conserved domain. These results suggest that RSV populations may evolve as complex and dynamic mutant swarms, despite apparent genetic stability

Drosophila Ric-8 regulates Gαi cortical localization to promote Gαi-dependent planar orientation of the mitotic spindle during asymmetric cell division

International audienceLocalization and activation of heterotrimeric G proteins have a crucial role during asymmetric cell division. The asymmetric division of the Drosophila sensory precursor cell (pI) is polarized along the antero-posterior axis by Frizzled signalling and, during this division, activation of Gαi depends on Partner of Inscuteable (Pins). We establish here that Ric-8, which belongs to a family of guanine nucleotide-exchange factors for Gαi, regulates cortical localization of the subunits Gαi and Gβ13F. Ric-8, Gαi and Pins are not necessary for the control of the antero-posterior orientation of the mitotic spindle during pI cell division downstream of Frizzled signalling, but they are required for maintainance of the spindle within the plane of the epithelium. On the contrary, Frizzled signalling orients the spindle along the antero-posterior axis but also tilts it along the apico-basal axis. Thus, Frizzled and heterotrimeric G-protein signalling act in opposition to ensure that the spindle aligns both in the plane of the epithelium and along the tissue polarity axis

Perturbative analysis of the effect of a magnetic field on gravito-inertial modes

Magnetic fields have been measured recently in the core of red giant stars thanks to their effects on stellar oscillation frequencies. The search for magnetic signatures in pulsating stars, such as $\gamma$ Doradus or Slowly Pulsation B stars, requires to adapt the formalism developed for the slowly rotating red giants to rapidly rotating stars. We perform a theoretical analysis of the effects of an arbitrary magnetic field on high radial order gravity and Rossby modes in a rapidly rotating star. The magnetic effects are treated as a perturbation. For high radial order modes, the contribution of the radial component of the magnetic field is likely to dominate over the azimuthal and latitudinal components. The rotation is taken into account through the traditional approximation of rotation. General expressions of the frequency shift induced by an arbitrary radial magnetic field are derived. Approximate analytical forms are obtained in the high-order high-spin-parameter limits for the modes most frequently observed in $\gamma$ Dor stars. We propose simple methods to detect seismic magnetic signatures and measure possible magnetic fields in such stars. These methods offer new possibilities to look for internal magnetic fields in future observations, such as the ones of the PLATO mission, or to revisit existing Kepler or TESS data.Comment: Accepted for publication in Astronomy & Astrophysics (18 pages, 18 figures