Use of natural caves during the Middle Bronze Age: new data from Southern Latium

Explorations conducted by speleological groups are a precious source of information on the use of natural caves in different periods, especially during the Metal Ages. Pottery shards dating from the Middle Bronze Age were recently found in southem Latium in the Caverna di San Pietro a Campea, the Grotta del Pistocchino and the Grotta La Sassa. Investigations by teams from the universities of Tor Vergata (Rome) and Groningen (Netherlands) in the La Sassa cave also discovered a Copper-Age burial area and a rich deposit of Pleistocene fauna

Low expression of estrogen receptor β in T lymphocytes and high serum levels of anti-estrogen receptor α antibodies impact disease activity in female patients with systemic lupus erythematosus

BACKGROUND: Current evidence indicates that estrogens, in particular 17β-estradiol (E2), play a crucial role in the gender bias of autoimmune diseases although the underlying molecular mechanisms have not yet been fully elucidated. Immune cells have estrogen receptors (ERs), i.e., ERα and ERβ, that play pro- and anti-inflammatory functions, respectively, and the presence of one estrogen receptor (ER) subtype over the other might change estrogen effects, promoting or dampening inflammation. In this study, we contributed to define the influences of E2 on T cells from female patients with systemic lupus erythematosus (SLE), a representative autoimmune disease characterized by a higher prevalence in women than in men (female/male ratio 9:1). Particularly, our aim was to evaluate whether alterations of ERα and ERβ expression in T cells from female SLE patients may impact lymphocyte sensitivity to E2 and anti-ERα antibody (anti-ERα Ab) stimulation interfering with cell signaling and display a direct clinical effect. METHODS: Sixty-one premenopausal female patients with SLE and 40 age-matched healthy donors were recruited. Patients were divided into two groups based on the SLE Disease Activity Index 2000 (SLEDAI-2K) (i.e., <6 and ≥6). ER expression was evaluated in T lymphocytes by flow cytometry, immunofluorescence, and Western blot analyses. Serum anti-ERα Ab levels were analyzed by enzyme-linked immunosorbent assay (ELISA). ER-dependent signaling pathways were measured by a phosphoprotein detection kit. RESULTS: Intracellular ERβ expression was significantly lower in T cells from patients with SLEDAI-2K ≥6 as compared with healthy donors and patients with SLEDAI-2K <6 and negatively correlated with disease activity. The expression of intracellular and membrane-associated-ERα was similar in SLE and control T cells. ER-dependent signaling pathways were activated in T cells from SLE patients with SLEDAI-2K ≥6, but not with SLEDAI-2K <6, when both membrane and intracellular ERs were stimulated by co-treatment with E2 and anti-ERα Abs. CONCLUSIONS: Our results demonstrate an altered ER profile in SLE patients, possibly contributing to SLE pathogenesis and interfering with clinical activity, and highlight the potential exploitation of T cell-associated ERβ as a biomarker of disease activity

The Pontine Marshes:An integrated study of the origin, history, and future of a famous coastal wetland in Central Italy

The Pontine Marshes (Central Italy) are known for their long drainage history starting early in Roman times and culminating in their reclamation (bonificaintegrale) by the Fascist’s regime under Mussolini, mostly in the 1930s of past century. The geology of this coastal wetland and causes for its drainageproblems received limited attention till recently. We reviewed results from recent studies, identified still existing knowledge gaps and performedadditional research to fill these, to produce a full description of the Holocene history and drainage of these marshes. Massive coring data that servedto map the soils and surficial geology, observations in archaeological excavations, analytical data on soil and sediment characteristics, and radiocarbondatings allowed us to distinguish several phases in this history, with a main break in the early Roman Republican period with the first systematic drainageworks. Earlier, natural processes brought about by sea level rise largely controlled its drainage. Once artificial drainage started, soil subsidence became animportant process, aggravating the drainage problems. The bonifica introduced mechanical drainage, temporarily masking the impacts of subsidence. Wequantified the historical subsidence using DTMs and developed scenarios for the impacts of sea level rise and subsidence in 2100 and 2200, assuming thatthis rise will be mitigated by a coastal defence system and enhanced capacity of the mechanical drainage. Our results demonstrate the important role ofsoil subsidence throughout the history of this wetland and need to include subsidence in scenarios for the impacts of sea level rise

Gram-negative septic thrombosis in critically ill patients: A retrospective case-control study

Background: Data on septic thrombosis caused by Gram-negative bacilli (GN-ST) in intensive care unit (ICU) patients are currently limited. Methods: The aim of this retrospective case–control study (matched 1:3) performed over a 15-month period on ICU patients with bacteraemia, associated (cases) or not (controls) with GN-ST, was to assess 30-day mortality and clinical/microbiological features of GN-ST. Results: During the study period, 16 patients with GN-ST and 48 controls were analyzed. Polytrauma was the cause of ICU admission in 12 (75%) cases and 22 (46%) controls (p = 0.019). In no case of septic thrombosis was surgical debridement performed. The site of venous thrombosis was more frequently in the lower limbs, associated with bone fracture in nine out of 12 (75%) cases. The median duration of bacteraemia (22 days vs 1 day; p 72 h was significantly associated with GN-ST (area under the curve (AUC) 0.95, sensitivity 0.996 and specificity 0.810; p < 0.001). Finally, 30-day mortality was 20% in cases and 67% in controls (p < 0.001). Conclusions: Critically ill patients with GN-ST showed specific clinical features. Despite delayed bacteraemia clearance, targeted antibiotic therapy plus anticoagulation usually provided clinical improvement and a low 30-day mortality rate