Applications of H. Dinges transformations in statistics

The work represent a tire exploiting model including both tire degradation and tire failures. The estimator of reliability characteristics: reliability function and the probability of k traumatic failure type were analysed. Natural tire wear and traumatic failure time data were analysed with SAS software

VARIATIONS OF STRUCTURE AND LEVELS OF HORMONES OF THE THYROID GLAND AND TESTERON WITH THE ERYTHROPOIESSTIMULATING ACTIONS

The object of investigation: he-rats of the Vistar line. The purpose of the work: to determine the dynamics of changes of the levels of erythropoetine, thyroid and male sexual hormones in the blood serum during the first 24 hours after the erythropoietic stimulation; to evaluate the degree of interconnection of reactions of the thyroid gland and gonades with the reaction of the erythron system to the erythropoisstimulating actions. The obligatory reduction of concentration of the hormones, to be produced by the thyroid gland, has been proved. The cause of this reduction has been differentiated, the forms and the terms of effect of the erythropoietic stimuli have been established, at which this reduction reflects the true supression of the thyroid gland or is the evidence of the high elimination of the hormones from the blood. The determination of the succession of reaction of the importent endocrine systems to the erythropoiesstimulating action is the base for searching the means intended for the normalization of the disturbed hemostasis. The method of the differential diagnostics and hormonal therapy of anaemias with the climacteric bleedings have been introduced in practice of the town hospitals number 8 and number 10 in Ryazan. The field of application: the hematology and the gynecologyAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Molecular and cellular mechanisms of acute cytotoxic liver damage as potential biological targets for magnesium-containing cell-protective drug

Introduction. Many anti-tumor drugs have a high potential for toxic damage to liver cells, which makes it necessary to identify molecular mechanisms of the development of the negative impact of drugs on the liver and to develop effective methods for preventing and correcting this adverse effect. Materials and methods. The study was performed on 30 nonlinear white rats of both sexes weighing 180–220 g, divided into 3 equal groups (n = 10 in each): intact control, control with liver pathology and experimental group of rats receiving the test substance LBK-527 at a dose of 100 mg/kg/day intragastrically one hour before the administration of a hepatoxic cytarabine. In the animals of the latter two groups, acute drug-induced hepatitis was simulated by intravenous administration of 2 g/m2 cytarabine in physiological saline for 5 days. Liver pathomorphology was studied on specimens stained with hematoxylin and eosin, Sudan III and by Van Gieson; a semi-quantitative method for assessing the depth of inflammatory and dystrophic organ damage was used. In the blood plasma, the activity of ASAT, ALAT, GGTP, and APF was determined. Tissue concentrations of TNF-alpha, IL-10 and HGF were determined by quantitative ELISA. Expression of Bcl-2 and Ki-67 was studied by immunohistochemistry. The proliferation index was calculated. Results and discussion. Daily administration of LBK-527 for 5 days restrains the depth of cytarabine-induced pathomorphological changes in the liver, reduces the prevalence of the dystrophic and inflammatory process, increases the anti-inflammatory and regenerative potential of the hepatic parenchyma, inhibits the programmed death of hepatocytes and reduces the activity of cytolytic and cholestatic syndromes. Conclusion. Magnesium-containing cell-protective substance LBK-527 protects liver from cytarabine-induced injury

ON THE QUESTION OF THE SAFETY OF A 4-ALKYL-SUBSTITUTED COMPOUND WITH ANTI-TUMOUR ACTION

The aim was to investigate the acute toxicity of the AX-554 compound following its intragastric administration, as well as to determine the effective cytotoxic concentration and dose of this preparation.Materials and methods. The study was performed using 76 nonlinear white laboratory mice of both sexes weighing 18–22 g, 150 male mice of C57BL/6 line and LCC tumour cell culture. The acute toxicity of the AX-554 4-alkyl-substituted compound in the form of granulated pellet mass was studied following its intragastric administration, with the results being analysed according to Litchfield and Wilcoxon. The effective dose of the substance was determined using a syngeneic tumour model in C57Bl/6 male mice with inoculated Lewis lung carcinoma. The effective concentration of the compound was determined in a tumour cell culture.Results. Our study of the acute toxicity of AH-554 after its intragastric administration in the form of granulate tablet mass have confirmed AH-554 to be a non-toxic substance. In doses ranging from 21.2 to 384 mg/kg, AH-554 is observed to suppress tumour growth in mice with syngeneic lung carcinoma at a level from 20 to 90%, with the highest therapeutic dose exceeding the minimum effective one by more than 18 times. This pattern is also observed when AH554 is applied in the culture of tumour cells. The results of this study can be used for developing a pharmaceutical based on the AH-554 compound.Conclusion. The AH-554 compound, 2-amino-4H-chromene derivative, is characterized by an optimal safety profile due its low toxicity and a wide range of anti-tumour action

Relationships of growth factors, proinflammatory cytokines, and anti-inflammatory cytokines with long-term clinical results of autologous bone marrow mononuclear cell transplantation in STEMI

AIM

Novel aminochromone derivative inhibits tumor growth on xenograft model of lung cancer in mice

2-Amino-4H-chromene derivatives possess anticancer property proved on different in vivo and in vitro models of malignancies such breast, nasopharyngeal, bladder, ovary carcinomas, astrocytoma, and osteosarcoma. We assumed it might be effective to apply one of the derivatives as promising approach to lung carcinoma treatment. to evaluate how novel 4-aryl substituted 2-amino-4H-chromene derivative AX-554 impacts tumor growth and progression, as well as possible mechanisms for anticancer effect development on in vivo patient-derived heterotopic xenograft model of lung carcinoma in mice. This was an experimental in vivo study. 40 nu/nu BALB/c female mice were randomly allocated into four equal groups: Intact, control, reference, and main group. Animals of three latter groups were ingrafted with human-derived lung adenocarcinoma. Antitumor and antimetastatic action of AX-554 novel aminochromone derivative as a substance were studied. Mice survival was registered. Kinase of anaplastic lymphoma (ALK), tubulin Beta-3 (TUBB3), and c-mesenchymal-epithelial transition (MET) concentrations in the prime tumor nodes homogenates were determined by quantitative enzyme-linked immunosorbent assay. Dannet's parametric criterion and the nonparametric exact Fisher test were used. The normality of the distribution was determined using ANOVA. The survival curve was analyzed using Gehan's criterion with the Yates's correction. Aminochromone derivative possesses an inhibitory effect on human lung adenocarcinoma transplanted into nu/nu BALB/c female mice, as well as significant antimetastatic activity. About 50 mg/kg/day AX-554 intragastric course increases animals' life expectancy of more than 3.3 times when compared with the control and induces remission in 60% of cases. The anticancer effect of the derivative is due to anti-ALK-mediated activation of tumor cells apoptosis and suppression TUBB3-dependent cell proliferation

Relationships of growth factors, proinflammatory cytokines, and anti-inflammatory cytokines with long-term clinical results of autologous bone marrow mononuclear cell transplantation in STEMI

<div><p>Aim</p><p>The aim of the study was to test the hypothesis suggesting that the pre-intervention levels of proinflammatory cytokines, anti-inflammatory cytokines, and angiogenic growth factors predict the long-term clinical results of autologous bone marrow-derived mononuclear cell (ABMMC) transplantation in patients with primary ST elevation myocardial infarction (STEMI).</p><p>Methods and results</p><p>From 2003 to 2006, a total of 62 patients with primary STEMI were enrolled in an open randomized study registered under the title ESTABOMA. Patients were randomized into two groups: group 1 included patients treated with percutaneous coronary intervention (PCI) and ABMMC transplantation (n = 28); group 2 comprised patients treated only with PCI (n = 34). Follow-up study was performed 7.96 ± 0.96 years after STEMI and involved physical examination, six-minute walk test, echocardiography, and determination of brain natriuretic peptide (BNP) levels. The total and cardiovascular mortality rates were higher in group 1 compared with group 2: 36% (n = 10) vs. 12% (n = 4) (p = 0.02) and 29% (n = 8) vs. 6% (n = 2) (p = 0.03), respectively. Lower levels of proinflammatory cytokines were observed in group 1 after PCI and ABMMC transplantation. Serum levels of FGF, VEGF, and IL-10, determined before PCI and ABMMC transplantation were prognostically significant long-term indicators of unfavorable course of CAD after STEMI.</p></div

ROC Curve for the IL-10 in group 1.

<p>ROC Curve for the IL-10 in group 1.</p

Results of the correlation analysis in group 2.

<p>Note: the statistically significant correlations of the cytokines and growth factors with the occurrences of endpoints in group 2 are shown in Fig 4. Correlation coefficients are placed over the arrows.</p