8 research outputs found
A Case Report of Breast Angiosarcoma
Angiosarcoma is a rare disease of the breast with the reported incidence of only 0.04% of all breast malignancies. The etiology of angiosarcoma remains unknown. It occurs post-mastectomy, in association with chronic lymphedema (Stewart-Treves syndrome), or after radiotherapy. We present a patient with angiosarcoma which developed 12 years of the diagnosis of breast carcinoma and 8 years of the operative procedure and radiotherapy for disease recurrence. A small angiomatous lesion of a few mm in size, cytologically suspect of vascular tumor (hemangioma or hemangiopericytoma) and histopathologically verified to be an atypical vascular lesion, was detected two years before breast enlargement and cytologic and histologic diagnosis of angiosarcoma. The patient died 15 months of the diagnosis of angiosarcoma, after two tumor recurrences and intrathoracic cavity invasion
T Lymphoblastic Leukaemia with an Unusual Burkitt Lymphoma Morphology ā A Case Report
Precursor T-cell acute lymphoblastic leukaemia (T-ALL)/lymphoma (T-LBL) is a neoplasm with cytological features that include blast cells of medium size, high nuclear cytoplasmic ratio and inconspicuous nucleoli, which are usually TdT (Terminal Deoxynucleotidyl Transferase) positive and variably express T-cell markers. We report a case of T-ALL with atypical cytological presentation which showed lymphoblasts with homogenous nuclear pattern, larger amounts of cytoplasm with vacuoles and prominent nucleoli. A 56-year-old male was hospitalized due to high fever and kidney infection. Further examination confirmed anemia, thrombocytopenia, normal level of white blood cells and high level of lactat-dehidrogenase (LDH). Bone marrow aspiration revealed 87% and peripheral blood 41% of lymphoblasts with cytoplasmic vacuoles which suggested Burkitt lymphoma (BL) morphology. Patientās karyotype showed no chromosomal aberations. Identification of immunophenotype discovered cells which were CD2 and CD3 positive and CD20 negative with focal acid phosphatase activity in 67% of blasts. This excluded Burkitt lymphoma and led to diagnosis of T-ALL. The patient was submitted to two cycles of chemotherapy, autologous stem cell transplantation, and intrathecal chemotherapy, but he died after 10 months because of disease complications (lung aspergillosis and pleural effusion). Our case report showed how morphology alone can be misleading and sometimes is not enough in diagnosing ALL. Beside morphologic criteria, setting correct diagnosis depends on identification of immunophenotype by flow cytometry and cytogenetic-molecular abnormalities. Further improvements in the molecular definition of ALL subtypes, development of new and targeted drugs will improve patientās outcome and prognosis
A Case Report of Breast Angiosarcoma
Angiosarcoma is a rare disease of the breast with the reported incidence of only 0.04% of all breast malignancies. The etiology of angiosarcoma remains unknown. It occurs post-mastectomy, in association with chronic lymphedema (Stewart-Treves syndrome), or after radiotherapy. We present a patient with angiosarcoma which developed 12 years of the diagnosis of breast carcinoma and 8 years of the operative procedure and radiotherapy for disease recurrence. A small angiomatous lesion of a few mm in size, cytologically suspect of vascular tumor (hemangioma or hemangiopericytoma) and histopathologically verified to be an atypical vascular lesion, was detected two years before breast enlargement and cytologic and histologic diagnosis of angiosarcoma. The patient died 15 months of the diagnosis of angiosarcoma, after two tumor recurrences and intrathoracic cavity invasion
Myelodysplastic syndromes ā new discoveries and an āoldā morphology
MijelodisplastiÄni sindrom heterogena je grupa bolesti koje dijele neka kliniÄka i morfoloÅ”ka obilježja. Uzrok im je nepoznat. BuduÄi da brz razvoj molekularne genetike omoguÄuje uvid u uzroÄne genske aberacije,
danas se mogu odrediti neki patogenetski mehanizmi u nastanku mijelodisplastiÄnog sindroma. U ovom radu pokuÅ”at Äemo objasniti neka otkriÄa u tom podruÄju i njihovu vezu s citomorfoloÅ”kim obilježjima, fenotipom mijelodisplastiÄnog sindroma.Myelodysplastic syndromes (MDS) are a heterogenous group of diseases sharing some clinical and morphological features. Their cause is unknown. Since the rapid development of molecular genetics allows an insight into underlying genetic aberrations, today it is possible to determine some pathogenetic mechanisms in MDS. In this presentation we would like to explain some of the discoveries in this area and their connection to the
myelodysplastic phenotype as seen in everyday cytomorphology work
Morphology of myelodysplastic/myeloproliferative neoplasms (MDS/MPN)
DijagnostiÄka kategorija mijelodisplastiÄne/mijeloproliferativne neoplazme (MDS/MPN) obuhvaÄa klonske hematopoetske neoplazme koje u vrijeme postavljanja dijagnoze istodobno pokazuju kliniÄka, laboratorijska i/ili morfoloÅ”ka obilježja i mijelodisplastiÄnog sindroma (MDS) i mijeloproliferativne neoplazme (MPN). Citopenija i displazija jedne ili viÅ”e mijeloidnih loza (obilježja MDS-a) mogu se vidjeti istodobno s leukocitozom, trombocitozom i/ili organomegalijom (obilježja koja su ÄeÅ”Äe povezana s MPN-om). Bolesnici s prije dijagnosticiranim MPN-om, kod kojih se razviju mijelodisplastiÄne promjene kao posljedica evolucije bolesti ili kemoterapije, ne ubrajaju se u ovu dijagnostiÄku kategoriju. Prema klasifikaciji Svjetske zdravstvene organizacije (engl. World Health Organization ā WHO) iz 2008. godine te njezinoj reviziji iz 2016. godine, MDS/MPN obuhvaÄa pet entiteta: kroniÄnu mijelomonocitnu leukemiju (engl. chronic myelomonocytic leukemia ā CMML), juvenilnu mijelomonocitnu leukemiju (engl. juvenile myelomonocytic leukemia ā JMML), atipiÄnu kroniÄnu mijeloiÄnu leukemiju, BCR-ABL1- (engl. atypical chronic myeloid leukemia ā aCML), MDS/MPN s prstenastim sideroblastima i trombocitozom (engl. MDS/MPN with ring sideroblasts and thrombocytosis ā MDS/MPN-RS-T) i neklasificirani MDS/MPN (engl. MDS/MPN, unclassifiable ā MDS/MPN, U).Diagnostic category of MDS/MPN includes clonal hematopoietic neoplasms, which show the concomitant clinical, laboratory and/or morphologic features of both myelodysplastic syndrome (MDS) and myeloproliferative neoplasm (MPN) at the time of diagnosis. Cytopenia and dysplasia of one or more myeloid lineages (the MDS features) can be present accompanied with leukocytosis, thrombocytosis and/or organomegaly (all features more often related to MPN). Patients with a previous diagnosis of MPN who develop the myelodysplastic alterations secondary to disease evolution or chemotherapy are not assigned to this diagnostic category. According to the WHO (World Health Organization) classification from 2008 and the 2016 revision, the MDS/MPN category includes five entities as follows: CMML (chronic myelomonocytic leukemia), JMML (juvenile myelomonocytic leukemia), aCML (atypical chronic myeloid leukemia) BCR-ABL1- , MDS/MPN-RS-T (MDS/MPN with ring sideroblasts and thrombocytosis) and MDS/MPN-U (MDS/MPN, unclassifiable)
T lymphoblastic leukaemia with an unusual Burkitt lymphoma morphology - a case report [T limfoblastiÄna leukemija sa neuobiÄajenom morfologijom Burkitovog limfoma - prikaz sluÄajeva]
Precursor T-cell acute lymphoblastic leukaemia (T-ALL)/lymphoma (T-LBL) is a neoplasm with cytological features that include blast cells of medium size, high nuclear cytoplasmic ratio and inconspicuous nucleoli, which are usually TdT (Terminal Deoxynucleotidyl Transferase) positive and variably express T-cell markers. We report a case of T-ALL with atypical cytological presentation which showed lymphoblasts with homogenous nuclear pattern, larger amounts of cytoplasm with vacuoles and prominent nucleoli. A 56-year-old male was hospitalized due to high fever and kidney infection. Further examination confirmed anemia, thrombocytopenia, normal level of white blood cells and high level of lactat-dehydrogenase (LDH). Bone marrow aspiration revealed 87% and peripheral blood 41% of lymphoblasts with cytoplasmic vacuoles which suggested Burkitt lymphoma (BL) morphology. Patient's karyotype showed no chromosomal aberations. Identification of immunophenotype discovered cells which were CD2 and CD3 positive and CD20 negative with focal acid phosphatase activity in 67% of blasts. This excluded Burkitt lymphoma and led to diagnosis of T-ALL. The patient was submitted to two cycles of chemotherapy, autologous stem cell transplantation, and intrathecal chemotherapy, but he died after 10 months because of disease complications (lung aspergillosis and pleural effusion). Our case report showed how morphology alone can be misleading and sometimes is not enough in diagnosing ALL. Beside morphologic criteria, setting correct diagnosis depends on identification of immunophenotype by flow cytometry and cytogenetic-molecular abnormalities. Further improvements in the molecular definition of ALL subtypes, development of new and targeted drugs will improve patient's outcome and prognosis
T limfoblastiÄna leukemija sa neuobiÄajenom morfologijom Burkitovog limfoma - prikaz sluÄajeva
Precursor T-cell acute lymphoblastic leukaemia (T-ALL)/lymphoma (T-LBL) is a neoplasm with cytological features that include blast cells of medium size, high nuclear cytoplasmic ratio and inconspicuous nucleoli, which are usually TdT (Terminal Deoxynucleotidyl Transferase) positive and variably express T-cell markers. We report a case of T-ALL with atypical cytological presentation which showed lymphoblasts with homogenous nuclear pattern, larger amounts of cytoplasm with vacuoles and prominent nucleoli. A 56-year-old male was hospitalized due to high fever and kidney infection. Further examination confirmed anemia, thrombocytopenia, normal level of white blood cells and high level of lactat-dehydrogenase (LDH). Bone marrow aspiration revealed 87% and peripheral blood 41% of lymphoblasts with cytoplasmic vacuoles which suggested Burkitt lymphoma (BL) morphology. Patient's karyotype showed no chromosomal aberations. Identification of immunophenotype discovered cells which were CD2 and CD3 positive and CD20 negative with focal acid phosphatase activity in 67% of blasts. This excluded Burkitt lymphoma and led to diagnosis of T-ALL. The patient was submitted to two cycles of chemotherapy, autologous stem cell transplantation, and intrathecal chemotherapy, but he died after 10 months because of disease complications (lung aspergillosis and pleural effusion). Our case report showed how morphology alone can be misleading and sometimes is not enough in diagnosing ALL. Beside morphologic criteria, setting correct diagnosis depends on identification of immunophenotype by flow cytometry and cytogenetic-molecular abnormalities. Further improvements in the molecular definition of ALL subtypes, development of new and targeted drugs will improve patient's outcome and prognosis.Prekursorska T-akutna limfoblastiÄna leukemija/limfom (T-ALL/LBL) je bolest morfoloÅ”ki karakterizirana srednje velikim limfoblastima s visokim omjerom jezgre i citoplazme i diskretnim nukleolima. Limfoblasti su pretežno TdT (Terminalna Deoksinukleotidil Transferaza) pozitivni uz razliÄito izražavanje T-staniÄnih biljega (CD1a, CD2, CD3, CD4, CD5, CD7 i CD8). Prikazujemo sluÄaj T-ALL-a atipiÄne citoloÅ”ke slike s limfoblastima, homogene strukture kromatina, izraženih nukleola te izrazito vakuolizirane citoplazme. 56-godiÅ”nji muÅ”karac je hospitaliziran zbog vruÄice i infekcije urotrakta. Dodatne pretrage pokazale su anemiju i trombocitopeniju te visoku razinu laktat-dehidrogenaze (LDH) uz uredan broj leukocita. U punktatu koÅ”tane srži na|eno je 87% a u perifernoj krvi 41% limfoblasta izrazito vakuolizirane citoplazme, Å”to je sugeriralo morfologiju Burkittovog limfoma (BL). Kariotip nije pokazao kromosomske aberacije. Imunofenotipski blasti su bili CD2 i CD3 pozitivni a CD20 negativni, s fokalnom citokemijskom pozitivnoÅ”Äu kisele fosfataze u 67% stanica. Na osnovu toga je iskljuÄen Burkittov limfom te je postavljena dijagnoza T-ALL-a. Nakon dva ciklusa kemoterapije pacijent je podvrgnut autolognoj transplantaciji koÅ”tane srži i intratekalnoj kemoterapiji. UnatoÄ provedenoj terapiji, bolesnik umire nakon 10 mjeseci zbog komplikacija bolesti (pluÄna aspergiloza i pleuralni izljev). NaÅ” sluÄaj pokazuje kako ponekad sama morfoloÅ”ka slika nije dovoljna za postavljenje dijagnoze ALL-a. Osim morfoloÅ”kih kriterija, za ispravnu dijagnozu neophodno je odrediti imunofenotip limfoblasta te prisutnost citogenetsko-molekularnih abnormalnosti. BuduÄi napretci u molekularnim definiranjima ALL subtipova i razvoj novih ciljanih lijekova poboljÅ”at Äe ishod i prognozu pacijenata