Breeding and Larval Rearing of Asian Moon Scallop Amusium pleuronectes in Eastern Samar, Philippines

Asian moon scallop, Amusium pleuronectes, is among the most common commercially harvested scallop in the Philippines. This study investigated suitable conditioning methods, induced spawning, and documented scallop larval development at the Marine Fisheries Research and Development Center (MFRDC) Guiuan, Eastern Samar. Scallops held in tanks with flow-through water system without substrate yielded high survival rate of 48.06 ± 5.95%. Optimum water temperature was 28-29°C for maintaining scallops. Isochrysis galbana and Chaetoceros calcitrans were suitable microalgal feed for A. pleuronectes consumed at 7,388,888 cells min-1 per scallop. These significant results were applied in maintaining scallops at the hatchery. As a result, scallops with an average 19.8 mm shell length (SL) reached 37.5 mm SL within four months with a survival rate of 96.57 ± 2.04%, average daily growth rate (ADGR) of 0.13 ± 0.04 mm.day-1, and specific growth rate (SGR) of 3.92 ± 1.31%.d-1. Natural spawning was successful under controlled conditions while induced spawning trial through thermal stimulation, food shock, sexual stimulation, and serotonin injection resulted unsuccessful release of sperm and eggs. The estimated number of fertilized eggs per spawning ranged from 0.22-1.4 million. Fertilized eggs appeared spherical and dark in color with 54.2-62.57 µm in diameter. After nine hours, the larvae developed into trochophore stage with 59.08-84.4 µm in length. D-veliger with 120.37-157.07µm shell length developed after 24 hours. Development of the early umbone stage was reached on day 5; and on day 7, the umbo larvae become well-developed with shell length of 135.45-173.36 µm. On day 9, pedi-veligers were observed in the culture. Spat grew 312.41-509.48 µm on day 16 and survived until four months with final shell length of 4-10 mm. For the larval rearing, stocking density of A. pleuronectes larvae observed highest survival rate of 0.04 ± 0.03% at 200 larvae/L, while no larvae survived at 800 larvae/L due to contamination of protozoans in the culture medium

Distribution and characterization of ampicillin- and tetracycline-resistant Escherichia coli from feedlot cattle fed subtherapeutic antimicrobials

<p>Abstract</p> <p>Background</p> <p>Feedlot cattle in North America are routinely fed subtherapeutic levels of antimicrobials to prevent disease and improve the efficiency of growth. This practice has been shown to promote antimicrobial resistance (AMR) in subpopulations of intestinal microflora including <it>Escherichia coli</it>. To date, studies of AMR in feedlot production settings have rarely employed selective isolation, therefore yielding too few AMR isolates to enable characterization of the emergence and nature of AMR in <it>E. coli </it>as an indicator bacterium. <it>E. coli </it>isolates (<it>n </it>= 531) were recovered from 140 cattle that were housed (10 animals/pen) in 14 pens and received no dietary antimicrobials (control - 5 pens, CON), or were intermittently administered subtherapeutic levels of chlortetracycline (5 pens-T), chlortetracycline + sulfamethazine (4 pens-TS), or virginiamycin (5 pens-V) for two separate periods over a 9-month feeding period. Phenotype and genotype of the isolates were determined by susceptibility testing and pulsed field gel electrophoresis and distribution of characterized isolates among housed cattle reported. It was hypothesized that the feeding of subtherapeutic antibiotics would increase the isolation of distinct genotypes of AMR <it>E. coli </it>from cattle.</p> <p>Results</p> <p>Overall, patterns of antimicrobial resistance expressed by <it>E. coli </it>isolates did not change among diet groups (CON vs. antibiotic treatments), however; isolates obtained on selective plates (i.e., M^A,M^T), exhibited multi-resistance to sulfamethoxazole and chloramphenicol more frequently when obtained from TS-fed steers than from other treatments. Antibiograms and PFGE patterns suggested that AMR <it>E. coli </it>were readily transferred among steers within pens. Most M^Tisolates possessed the <it>tet</it>(B) efflux gene (58.2, 53.5, 40.8, and 50.6% of isolates from CON, T, TS, and V steers, respectively) whereas among the M^A(ampicillin-resistant) isolates, the <it>tem1</it>-like determinant was predominant (occurring in 50, 66.7, 80.3, and 100% of isolates from CON, T, TS, and V steers, respectively).</p> <p>Conclusions</p> <p>Factors other than, or in addition to subtherapeutic administration of antibiotics influence the establishment and transmission of AMR <it>E. coli </it>among feedlot cattle.</p

Growth, Development, and Survival of Portunus pelagicus Larvae and Juveniles in Different Feed Regimens, Rearing Media, and Stocking Densities

Various factors in P. pelagicus seed production and grow-out culture were investigated in this study. Experiments were conducted to 1) compare natural and artificial feeds for larval production, 2) evaluate indoor tank and outdoor net cages as rearing media, and 3) assess different stocking densities for grow-out culture. Growth, development, and survival were assessed for the performance of each variable. (I) In larval production, the development of megalopa larvae into crab instar was synchronous in both natural and artificial feed treatments in a 5-day rearing observation. Crab instars began to appear on Day 4 with 27% composition in both feeds. Although survival appeared to be relatively higher in natural feed (43.96 ± 6.04%), this was not significant from survival in artificial feed treatment (33.33 ± 13.34), (t = 0.726, p > 0.05). (II) In Phase I grow-out culture, a two-variable design experiment was conducted to assess growth performance and survival of crab juveniles reared in indoor tanks and outdoor net cages at different stocking densities. Specific growth rate (SGR) differed significantly (t = 2.937, p 0.05). Furthermore, two-factor ANOVA results have shown that growth performance of the juveniles was influenced by both the differences in rearing medium and stocking density, but not in terms of survival (F = 0.120, p = 0.888). (III) In Phase II grow-out culture, juveniles attained highest SGR (3.54 ± 0.56%.d-1) at 5 ind.m-2 stocking density. This was followed by 15 ind.m-2 (3.45 ± 2.39%.d-1) and by 10 ind.m-2 (2.33 ± 0.50%.d-1) (p > 0.05). However, survival rate was highest in 15 ind.m-2 (46.67 ± 0.00%), but the differences among other stocking densities were not statistically significant (p > 0.05). Overall, results suggest that artificial feed can be an alternative for Artemia in rearing megalopae to crab instar stage. Stocking density in Phase I and II grow-out culture did not substantially affect growth performance and survival of juvenile P. pelagicus. However, higher stocking density increases incidence of cannibalism among reared crabs

Terminology of Geological Time: Establishment of a Community Standard

It has been recommended that geological time be described in a single set of terms and according to metric or SI (“Système International d’Unités”) standards, to ensure “worldwide unification of measurement”. While any effort to improve communication in scientific research and writing is to be encouraged, we are also concerned that fundamental differences between date and duration, in the way that our profession expresses geological time, would be lost in such an oversimplified terminology. In addition, no precise value for ‘year’ in the SI base unit of second has been accepted by the international bodies.Under any circumstances, however, it remains the fact that geological dates – as points in time – are not relevant to the SI. Known dates may define durations, just as known durations may define dates, or dates may simply be punctual references that support historical narratives, but dates are not quantities. Furthermore, dates, as datum points, belong to a specific type of guiding information that is in constant use not only by the disciplines that explore the unwritten past, but in the physical sciences and engineering as well. Accordingly, we recommend a new standardization of the distinction between geohistorical date, in years before present expressed in ‘annus’, symbol ‘a’,with the multiples ‘ka’, ‘Ma’, and ‘Ga’ for thousands, millions and billions of years ago, according to a convention that has been very widely adopted during the last 30 years, and geohistorical duration, expressed in ‘year’, symbol ‘yr’, with multiples ‘kyr’, ‘Myr’ and ‘Gyr’, respectively, as the most appropriate among the various formats in the current literature. Agreement on these two sets of terms throughout the wide community that deals with paleochronology would remove a false impression of improvisation and uncertainty as to appropriate terminology, and would lead to more effective communication in areas where a simplified but needlessly SI-conisistent terminology would be less, not more useful