Expression of plasma prekallikrein mRNA in human nonhepatic tissues and cell lineages suggests special local functions of the enzyme

At present it is generally accepted that plasma prekallikrein (PPK) is synthesized in the liver and secreted into the bloodstream. Surprisingly, it has recently been shown that PPK mRNA is present also in RNA from the kidney, adrenal gland and placenta. In spite of its novelty and possible important physiological implications this finding has been neglected. Here we report that PPK mRNA is expressed also in the human brain, heart, lung, trachea, endothelial cells and leukocytes as well as in a variety of fibroblast and epithelial cell lines. Expression of PPK mRNA in fibroblasts, endothelial cells and leukocytes suggests that PPK mRNA detected in RNA preparations from whole tissue may originate solely from these ubiquitously occurring cells. However, PPK mRNA expression in various epithelial cell lines demonstrates that tissue-specific cells also transcribe the PPK gene. The presence of PPK mRNA in nonhepatic tissues and cells indicates that they have the capacity to synthesize the protein. The physiological role of PPK synthesized in extrahepatic tissue is unknown. It may participate in local actions within tissues as well as contributing to the PPK pool in blood plasma. Cultured cells will provide a valuable model for exploring the physiological significance of extrahepatic PPK expression

Is Ridge Cultivation Sustainable? A Case Study from the Haean Catchment, South Korea

Non-sustainable agricultural practices can alter the quality of soil and water. A sustainable soil management requires detailed understanding of how tillage affects soil quality, erosion, and leaching processes. Agricultural soils in the Haean catchment (South Korea) are susceptible to erosion by water during the monsoon. For years, erosion-induced losses have been compensated by spreading allochthonous sandy material on the fields. These anthropogenically modified soils are used for vegetable production, and crops are cultivated in ridges using plastic mulches. To evaluate whether the current practice of ridge cultivation is sustainable with regard to soil quality and soil and water conservation, we (i) analysed soil properties of topsoils and (ii) carried out dye tracer experiments. Our results show that the sandy topsoils have a very low soil organic matter content and a poor structure and lack soil burrowers. The artificial layering induced by spreading sandy material supported lateral downhill water flow. Ridge tillage and plastic mulching strongly increased surface runoff and soil erosion. We conclude that for this region a comprehensive management plan, which aims at long-term sustainable agriculture by protecting topsoils, increasing soil organic matter, and minimizing runoff and soil erosion, is mandatory for the future

Utilization of Polyspecific Antiserum for Specific Radioimmunoassays: Radioimmunoassays for Rat Fetuin and Bikunin Were Developed by Using Antiserum Against Total Rat Serum Proteins

Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. The radioimmunoassays proved to be highly useful to trace bikunin and fetuin in the course of developing isolation procedures, since neither specific functional assays nor monospecific antisera were available. The two examples demonstrate that, in general, it will be possible to develop a specific and sensitive radioimmunoassay with antiserum raised against a crude antigen preparation, such as a body fluid or a tissue extract, provided that a minute amount of pure antigen is available for preparing the radioiodinated antigen