56 research outputs found
Molecular cloning of the rat proteinase-activated receptor 4 (PAR4)
BACKGROUND: The proteinase-activated receptor 4 (PAR4) is a G-protein-coupled receptor activated by proteases such as thrombin and trypsin. Although activation of PAR4 has been shown to modulate rat gastrointestinal motility, the rat PAR4 sequence was unknown until now. This study aimed to identify the rat PAR4 cDNA. RESULTS: The cDNA coding for the rat PAR4 homologue was cloned from the duodenum. Northern blots demonstrated a 3.0 kb transcript in the duodenum. Protein homology with mouse and human counterparts was 90% and 75% respectively. PAR4 is expressed predominantly in the esophagus, stomach, duodenum and the spleen. When expressed in COS cells, PAR4 is activated by trypsin (1 nM), thrombin (50 nM), mouse PAR4 specific peptide (500 ÎĽM) and a putative rat PAR4 specific activating peptide (100 ÎĽM), as measured by intracellular Ca(2+)-changes. CONCLUSIONS: We have identified and characterized cDNA encoding the rat PAR4 homologue. PAR4 is expressed predominantly in the upper gastrointestinal tract. It is activated by trypsin, thrombin and its newly identified rat PAR4 specific activating peptide
Successful transplantation of neuronal stem cells in the gastrointestinal tract with evidence of morphological and functional differentiation
262 Identification and Characterization of a Subset of Enteric “Glial” Cells As Neural Precursors
T1737 Making Enteric Neurons out of Fibroblasts: A Novel Approach Using Small Molecule Inhibitor of ROCKI/II
T1736 An In Vitro Model to Study the Potential of Neural Stem Cells of Diverse Origins to Assume an Enteric Phenotype
Role of enteric nerves in the regulation and propagation of spontaneous and electrically “paced” gastric slow waves in rats
NGF expression and release in experimental and human pancreatitis and its potential role in the pathogenesis of pain in chronic pancreatitis
751 Collagen IV-Pten Signaling Regulates Adult Enteric Neurogenesis In Vitro and In Vivo
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