Guidelines for the use and interpretation of diagnostic methods in adult food allergy

Food allergy has an increasing prevalence in the general population and in Italy concerns 8 % of people with allergies. The spectrum of its clinical manifestations ranges from mild symptoms up to potentially fatal anaphylactic shock. A number of patients can be diagnosed easily by the use of first- and second-level procedures (history, skin tests and allergen specific IgE). Patients with complex presentation, such as multiple sensitizations and pollen-food syndromes, frequently require a third-level approach including molecular diagnostics, which enables the design of a component-resolved sensitization profile for each patient. The use of such techniques involves specialists' and experts' skills on the issue to appropriately meet the diagnostic and therapeutic needs of patients. Particularly, educational programs for allergists on the use and interpretation of molecular diagnostics are needed

Developing complete event scenarios starting from lifelines damage assessment

Riferimento paper: 51

PROVENANCE, DISTRIBUTION AND TRADE OF THE LOCAL BUILDING MATERIALS IN THE SARNO RIVER PLAIN (CAMPANIA) FROM THE 6TH CENTURY BC TO AD 79

A multidisciplinary research project about the reconstruction of the ancient cultural landscape of the Sarno River Plain before the eruption of Somma-Vesuvius AD 79 was initiated in 2006 by the German Archaeological Institute. As a part of this research this study investigates the natural resources in the hinterland of Pompeii, Nuceria and Stabiae, used for building purposes. Stone extraction was practiced mainly along the foot slope of Somma-Vesuvius and the Apennines. Carbonates and Campanian Ignimbrite could even have been quarried in littoral quarries along the Sorrento Peninsula. Possible areas of stone extraction overlap the main ancient traffic routes. A high number of quarries of small or medium dimension can be inferred. In the centuries of urbanisation of the area (from the 6th century onwards) resources preferably close to the settlements and rather soft stony materials were preferred. Later on, even local hard rocks were used. During the latest decades of the Late Republic import of building materials is clearly increasing, mainly from other parts of Campania

DSA e memoria di lavoro

Oggi sono sempre di pi\uf9 gli studenti con DSA che terminata la scuola secondaria di secondo grado, desiderano iscriversi all\u2019universit\ue0 e che cercano di inserirsi in contesti lavorativi in linea con i propri studi e le proprie aspirazioni. Questo \ue8 sicuramente un segnale positivo, soprattutto per la crescente attenzione da parte del mondo accademico, anche se rimane ancora molto da fare per approdare a una completa inclusione. I contributi presenti in questo volume, attraverso un\u2019ottica multidisciplinare, indagano quali siano le misure dispensative e gli strumenti compensativi da utilizzare, le buone prassi da seguire, le possibili prospettive lavorative e le strategie pi\uf9 efficaci per favorire il successo formativo di ragazzi e giovani con DSA

Kinematic, bioenergetic and oxidative evaluations of donkey sperm preserved at +4°C

SummaryInformation on donkey sperm bioenergetics, kinetics and oxidative status is scarce even though crucial for development of reproductive technologies and germplasm conservation. For these reasons, it is interesting to monitor sperm kinetics, bioenergetics, and oxidative status during sperm storage at +4°C and with several sperm extenders and concentrations. Donkey semen was collected from three jackasses, three times each. It was diluted with four extenders (Kenney, Equiplus, INRA96 or Hippex), set at three sperm concentrations (30, 50 or 70 × 106 spermatozoa/ml) and evaluated for its functionality after 0, 3, 24, 48 and 72 h storage at +4°C. Sperm kinetics was analyzed by Sperm Computer Analysis; sperm bioenergetics was assessed by mitochondrial membrane potential (MMP); sperm oxidative status was evaluated by lipid peroxidation (LPO), anti-LPO potential and nitroblue tetrazolium (NBT) assays. Incubation produced a progressive (P < 0.01) decline in sperm kinetics and MMP, whereas parameters related to oxidative status either increased (LPO, NBT) or decreased (anti-LPO). The anti-LPO potential was the index better related to sperm motility and kinetics. Extenders proved to be differently (P < 0.01) effective in preserving sperm kinetics, MMP, and oxidative status. The concentration of 30 × 106 spermatozoa/ml provided an optimum preservation of sperm functions. Significant correlations emerged between most parameters examined. This study identified reference criteria for storing donkey spermatozoa at +4°C. A low sperm concentration together with a proper extender are crucial requirements for optimum sperm cryopreservation efficiency. Field trials are, however, required to validate these findings, making them operational in practice