Neighborhoods of Fortaleza where collections of <i>Aedes spp</i> were carried out.

<p>DENV was isolated from <i>Aedes</i> mosquitoes in the neighborhoods colored in blue.</p

Records of the occurrence of vertical transmission of DENV in <i>A. aegypti</i> and <i>A. albopictus</i>.

a<p>Adults that emerged from larvae collected in the field;</p>b<p>Adults that emerged from eggs collected in the field;</p>c<p>Adults collected in the field;</p><p>(*)No information available on the sex of the mosquitoes tested;</p><p>(♦)Serotypes not specified.</p

Indirect fluorescent antibody test of <i>Aedes</i> mosquitoes pools.

<p>Pools 34 (A), 35 (B) and 49 (C) were positive for DENV; NC = negative control.</p

Infestation rates of neighborhoods of Fortaleza and pools of <i>A. aegypti</i> and <i>A. albopictus</i> females submitted to IFA and RT-PCR.

*<p>Mosquitoes/pool – month and year of collection. In bold, positive pools by both IFA and RT-PCR. The other pools were negative for both tests.</p

Electrophoresis in agarose gel of the products of RT-PCR.

<p>Wells 1, 2 and 3 indicate negative pools for the dengue virus; well 4 reveals genomic fragment of DENV -3, obtained from the pool 34; well 5 reveals genomic fragment of DENV-2 obtained from the pool 35; well 6 reveals simultaneously genomic fragments of DENV-2 and DENV-3, obtained from the pool 49; well 7 contains the negative control, and well 8 contains a mix of DENV-1, DENV-2 and DENV-3 (positive control).</p