GPER overexpression in cervical cancer versus premalignant lesions: Its activation induces different forms of cell death

Background: The effect of estrogen has been traditionally studied through the modulation of its alpha and beta nuclear receptors; however, the G Protein-Coupled Estrogen Receptor (GPER) has been recently involved in the pathology of numerous tumors. Although the study of GPER in cervical cancer has begun, its contribution still remains to be completely evaluated. Objective: The purpose of this work was to determine the expression of this receptor in different degrees of cervical lesions and whether the stimulation with its specific agonist (G-1) modulated mechanisms of cell survival or cell death in cervical cancer cells. Methods: Sections of 44 formalin-fixed paraffin-embedded blocks from patients were analyzed by automated immunohistochemistry. After the stimulation with G-1, proliferation was evaluated by the xCELLigence technology, the integrity of the mitochondrial membrane permeability by MitoCapture™ fluorescence staining, apoptosis by flow cytometry, and senescence by the senescence-associated β-galactosidase kit. Results: GPER was widely expressed in cervical cancer but not in its precursor lesions. The staining was predominantly cytoplasmic, although it was also important in the nucleus of the epithelial cells. G-1 inhibited proliferation, decreased the mitochondrial permeability, and increased the percentage of apoptosis in SiHa, HeLa, and C-33A. Only in C-33A, an increase of the cells in necrosis was observed, whereas SiHa was the only cell line in which senescence was evidenced. Conclusion: GPER is a receptor associated with cervical cancer that inhibits the growth and induces different mechanisms of death in cells derived from uterine cervical cancer. It suggests that GPER can be considered a pharmacological target that prevents the development of cervical carcinogenesis.Fil: Silva, Christian Oscar. Universidad de Guadalajara; MéxicoFil: Leal, Annie R.. Universidad de Guadalajara; MéxicoFil: Ortiz Lazareno, Pablo C.. Instituto Mexicano del Seguro Social; MéxicoFil: Suárez, Luis F. J.. Instituto Mexicano del Seguro Social; MéxicoFil: de Arellano, Adrián R.. Universidad de Guadalajara; MéxicoFil: Lopez Pulido, Edgar I.. Universidad de Guadalajara; MéxicoFil: Barragan, José G. M.. Universidad de Guadalajara; MéxicoFil: Buelna, Margarita M.. Universidad de Guadalajara; MéxicoFil: Rodríguez, Judith R. D.. Hospital Civil de Guadalajara; MéxicoFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Muñoz Valle, José F.. Instituto Mexicano del Seguro Social; MéxicoFil: Pereira Suárez, Ana L.. Universidad de Guadalajara; Méxic

Estudios culturales : prácticas diversas, enfoques pluralistas

Desde el ámbito de los estudios culturales plantea un acercamiento teórico y metodológico multidisciplinar en el que a partir de la reflexión y el estudio de casos se analizan problemáticas reales de la historia de la cultura mexicana. Ciencia y humanismo, empirismo y reflexión son las caras de la moneda de una tendencia epistemológica que en las últimas décadas se ha planteado en el ámbito de la investigación sociológica y antropológica bajo el marbete de estudios culturales, con el objetivo de aprehender el complejo funcionamiento de los procesos culturales en las sociedades modernas