Recovery using “float” from high intensity stress on growth hormone-like molecules in resistance trained men

Objective The purpose of this study was to examine the influence of a novel “floatation-restricted environmental stimulation therapy” (floatation-REST) on growth hormone responses to an intense resistance exercise stress. Design Nine resistance trained men (age: 23.4 ± 2.5 yrs.; height: 175.3 ± 5.4 cm; body mass: 85.3 ± 7.9 kg) completed a balanced, crossover-controlled study design with two identical exercise trials, differing only in post-exercise recovery intervention (i.e., control or floatation-REST). A two-week washout period was used between experimental conditions. Plasma lactate was measured pre-exercise, immediately post-exercise and after the 1 h. recovery interventions. Plasma iGH was measured pre-exercise, immediately-post exercise, and after the recovery intervention, as well as 24 h and 48 h after the exercise test. The bGH-L was measured only at pre-exercise and following each recovery intervention. Results For both experimental conditions, a significant (P ≤ 0.05) increase in lactate concentrations were observed immediately post-exercise (~14 mmol • L-1) and remained slightly elevated after the recovery condition. The same pattern of responses was observed for iGH with no differences from resting values at 24 and 48 h of recovery. The bGH-L showed no exercise-induced changes following recovery with either treatment condition, however concentration values were dramatically lower than ever reported. Conclusion The use of floatation-REST therapy immediately following intense resistance exercise does not appear to influence anterior pituitary function in highly resistance trained men. However, the lower values of bGH suggest dramatically different molecular processing mechanisms at work in this highly trained population

The betaine content of sweat from adolescent females

<p>Abstract</p> <p>Background</p> <p>This study was developed to establish whether betaine was present in the sweat of females and to determine any correlations with other sweat components.</p> <p>Methods</p> <p>Sweat patches were placed on eight trained adolescent Highland dancers (age = 13.6 ± 2.3 yr), who then participated in a dance class for 2 hours. Patches were removed, and the sweat recovered via centrifugation. The sweat was subsequently analyzed for betaine, choline, sodium, potassium, chloride, lactate, glucose, urea and ammonia.</p> <p>Results</p> <p>Betaine was present in the sweat of all subjects (232 ± 84 μmol·L^-1), which is higher than typically found in plasma. The concentration of several sweat components were correlated, in particular betaine with most other measured components.</p> <p>Conclusion</p> <p>Betaine, an osmoprotectant and methyl donor, is a component of sweat that may be lost from the body in significant amounts.</p

Dietary calcium intake and Renin Angiotensin System polymorphisms alter the blood pressure response to aerobic exercise: a randomized control design

BACKGROUND: Dietary calcium intake and the renin angiotensin system (RAS) regulate blood pressure (BP) by modulating calcium homeostasis. Despite similar BP regulatory effects, the influence of dietary calcium intake alone and combined with RAS polymorphisms on the BP response following acute aerobic exercise (i.e., postexercise hypotension) has not been studied. Thus, we examined the effect of dietary calcium intake and selected RAS polymorphisms on postexercise hypotension. METHODS: Subjects were men (n = 50, 43.8 ± 1.3 yr) with high BP (145.3 ± 1.5/85.9 ± 1.1 mm Hg). They completed three experiments: non-exercise control and two cycle bouts at 40% and 60% of maximal oxygen consumption (VO(2)max). Subjects provided 3 d food records on five protocol-specific occasions. Dietary calcium intake was averaged and categorized as low (<880 mg/d = LowCa) or high (≥ 880 mg/d = HighCa). RAS polymorphisms (angiotensin converting enzyme insertion/deletion, ACE I/D; angiotensin II type 1 receptor, AT(1)R A/C) were analyzed with molecular methods. Genotypes were reduced from three to two: ACE II/ID and ACE DD; or AT(1)R AA and AT(1)R CC/AC. Repeated measure ANCOVA tested if BP differed among experiments, dietary calcium intake level and RAS polymorphisms. RESULTS: Systolic BP (SBP) decreased 6 mm Hg after 40% and 60% VO(2)max compared to non-exercise control for 10 h with LowCa (p < 0.01), but not with HighCa (p ≥ 0.05). Under these conditions, diastolic BP (DBP) did not differ between dietary calcium intake levels (p ≥ 0.05). With LowCa, SBP decreased after 60% VO(2)max versus non-exercise control for 10 h among ACE II/ID (6 mm Hg) and AT(1)R AA (8 mm Hg); and by 8 mm Hg after 40% VO(2)max among ACE DD and AT(1)R CC/CA (p < 0.01). With HighCa, SBP (8 mm Hg) and DBP (4 mm Hg) decreased after 60% VO(2)max compared to non-exercise control for 10 h (p < 0.05), but not after 40% VO(2)max (p ≥ 0.05). CONCLUSION: SBP decreased after exercise compared to non-exercise control among men with low but not high dietary calcium intake. Dietary calcium intake interacted with the ACE I/D and AT(1)R A/C polymorphisms to further modulate postexercise hypotension. Interactions among dietary calcium intake, exercise intensity and RAS polymorphisms account for some of the variability in the BP response to exercise

Resistance Training and Milk-Substitution Enhance Body Composition and Bone Health in Adolescent Girls

Background: Increased soft-drink consumption has contributed to poor calcium intake with 90% of adolescent girls consuming less than the RDA for calcium. Purpose/objectives: The purpose of this investigation was to determine the independent and additive effects of two interventions (milk and resistance training) on nutrient adequacy, body composition, and bone health in adolescent girls. Methods: The experimental design consisted of four experimental groups of adolescent girls 14–17 years of age: (1) Milk + resistance training [MRT]; n = 15; (2) Resistance training only [RT]; n = 15; (3) Milk only [M] n = 20; (4) Control [C] n = 16. A few significant differences were observed at baseline between the groups for subject characteristics. Testing was performed pre and post-12 week training period for all groups. Milk was provided (3, 8 oz servings) for both the MRT and the M groups. The MRT group and the RT groups performed a supervised periodized resistance training program consisting of supervised one-hour exercise sessions 3 d/wk (M, W, F) for 12 wk. Baseline dietary data was collected utilizing the NUT-P-FFQ and/or a 120 item FFQ developed by the Fred Hutchinson Cancer Research Center (Seattle, Washington). Body composition was measured in the morning after an overnight fast using dual-energy X-ray absorptiometry (DXA) with a total body scanner (ProdigyTM, Lunar Corporation, Madison, WI). A whole body scan for bone density and lumbar spine scans were performed on all subjects. Maximal strength of the upper and lower body was assessed via a one-repetition maximum (1-RM) squat and bench press exercise protocols. Significance was set at P ≤ 0.05. Results: Significant differences in nutrient intakes between groups generally reflected the nutrient composition of milk with greater intakes of protein and improved nutrient adequacy for several B vitamins, vitamin A, vitamin D, calcium, magnesium, phosphorus, potassium, and zinc. Mean calcium intake was 758 and 1581 mg/d, in the non-milk and milk groups, respectively, with 100% of girls in the milk groups consuming \u3e RDA of 1300 mg/d. There were no effects of milk on body composition or muscle performance, but resistance training had a main effect and significantly increased body mass, lean body mass, muscle strength, and muscle endurance. There was a main effect of milk and resistance training on several measures of bone mineral density (BMD). Changes in whole body BMD in the M, RT, MRT, and CON were 0.45, 0.52, 1.32, and −0.19%, respectively (P \u3c 0.01). Conclusions: Over the course of 12 weeks the effects of 1300 mg/d of calcium in the form of fluid milk combined with a heavy resistance training program resulted in the additive effects of greater nutrient adequacy and BMD in adolescent girls. While further studies are needed, combining increased milk consumption with resistance training appears to optimize bone health in adolescent girls

Ergogenic effects of betaine supplementation on strength and power performance

<p>Abstract</p> <p>Background</p> <p>We investigated the ergogenic effects of betaine (B) supplementation on strength and power performance.</p> <p>Methods</p> <p>Twelve men (mean ± SD age, 21 ± 3 yr; mass, 79.1 ± 10.7 kg) with a minimum of 3 months resistance training completed two 14-day experimental trials separated by a 14-day washout period, in a balanced, randomized, double-blind, repeated measures, crossover design. Prior to and following 14 days of twice daily B or placebo (P) supplementation, subjects completed two consecutive days (D1 and D2) of a standardized high intensity strength/power resistance exercise challenge (REC). Performance included bench, squat, and jump tests.</p> <p>Results</p> <p>Following 14-days of B supplementation, D1 and D2 bench throw power (1779 ± 90 and 1788 ± 34 W, respectively) and isometric bench press force (2922 ± 297 and 2503 ± 28 N, respectively) were increased (p < 0.05) during REC compared to pre-supplementation values (1534 ± 30 and 1498 ± 29 W, respectively; 2345 ± 64 and 2423 ± 84 N, respectively) and corresponding P values (1374 ± 128 and 1523 ± 39 W; 2175 ± 92 and 2128 ± 56 N, respectively). Compared to pre-supplementation, vertical jump power and isometric squat force increased (p < 0.05) on D1 and D2 following B supplementation. However, there were no differences in jump squat power or the number of bench press or squat repetitions.</p> <p>Conclusion</p> <p>B supplementation increased power, force and maintenance of these measures in selected performance measures, and these were more apparent in the smaller upper-body muscle groups.</p

Unos L-alanil-L-glutamina tijekom kratkotrajne visokointenzivne vježbe u uvjetima blagoga hidracijskoga stresa

The effect of acute L-alanyl-L-glutamine (AG) ingestion on selected hormonal and electrolyte measures was examined during repetitive, short duration, high intensity exercise with mild hypohydration. Subjects (20.3±1.1 yrs; 180.3±10.4 cm; 83.1±14.0 kg; 11.6±3.6% body fat) reported to the Human Performance Laboratory on four occasions. During each trial subjects were hypohydrated to -2.5% of their baseline body mass. During one trial (DHY) subjects rested in a recumbent position for 45 minutes before commencing the exercise session. During the other three trials subjects were rehydrated to 1.5% of their baseline body mass, before exercise, by drinking water only (W), or with two different doses of AG – a low dose (LDAG: 0.05 g�kg-1) and a high dose (HDAG: 0.2 g�kg-1). The exercise protocol consisted of ten 10-second sprints on a cycle ergometer with a 1-min rest between each sprint. Blood draws were collected once the subject achieved the desired level of hypohydration, immediately pre-exercise, immediately post-exercise, and 24 hrs postexercise. Blood samples were analyzed for glutamine, potassium, sodium, aldosterone, arginine vasopressin, C-reactive protein, interleukin-6, malondialdehyde, testosterone, cortisol, ACTH, and growth hormone. The area under the curve (AUC) analysis demonstrated significantly greater sodium concentrations for DHY compared to all other trials. The AUC analysis for aldosterone showed significantly lower concentrations at LDAG compared to DHY. No other differences between trials were observed in any other hormonal or biochemical responses. AG ingestion during a short duration, anaerobic exercise and mild hypohydration stress had a limited effect on selected hormonal and biochemical measures.Učinci akutnoga, trenutačnoga uzimanja L-alanil-L-glutamina (AG) na odabrane hormonske i elektrolitne pokazatelje ispitani su tijekom ponavljajuće kratkotrajne visokointenzivne aktivnosti u uvjetima blage hipohidracije ispitanika. Ispitanici (20,3±1,1 godina; 180,3±10,4 cm; 83,1±14,0 kg; 11,6±3,6% tjelesne masti) bili su testirani u Human Performance Laboratory u četiri navrata. Tijekom svakoga pojedinačnoga mjerenja ispitanici su bili hipohidrirani do -2,5% svoje početne, osnovne tjelesne mase. Tijekom prvoga testiranja (DHY) ispitanici su se odmarali ležeći 45 minuta prije no što su počeli provoditi protokol vježbanja. Tijekom sljedeća tri mjerenja ispitanici su rehidrirani do 1,5% njihove početne tjelesne mase prije vježbanja, i to: pijenjem samo vode (W) te unosom dviju različitih doza AG - male doze (LDAG: 0,05 g∙kg-1) i velike doze (HDAG: 0,2 g∙kg-1). Protokol vježbanja sastojao se od po deset sprintova na bicikl-ergometru u trajanju od 10 sekunda s jednominutnim odmorom između svakoga sprinta. Uzorci krvi vađeni su odmah nakon što je ispitanik dosegao željenu razinu hipohidracije, neposredno prije početka vježbanja, neposredno nakon završetka vježbanja i 24 sata nakon vježbanja. U uzorcima krvi analizirana je koncentracija glutamina, kalija, natrija, aldosterona, arginin vazopresina, C-reaktivnoga proteina, interleukina-6, malondialdehida, testosterona, kortizola, ACTH-a i hormona rasta. Analiza površine ispod krivulje pokazala je statistički značajno veću razinu koncentracije natrija u ispitanika u prvom testu (DHY) u odnosu na sva ostala mjerenja. Analiza površine ispod krivulje za aldosteron je pokazala značajno nižu koncentraciju u testu LDAG u odnosu na test DHY. Nisu zapažene značajne razlike između pojedinih mjerenja ni u jednoj drugoj hormonskoj i biokemijskoj reakciji na protokol vježbanja. Uzimanje AG tijekom kratkotrajne anaerobne aktivnosti i u stanju blagoga hipohidracijskoga stresa pokazalo je ograničene učinke na odabrane hormonske i biokemijske pokazatelje

Changes of Hydration Measures in Elite National Collegiate Athletic Association Division I Wrestlers

Purpose: To evaluate the changes in the state of hydration in elite National Collegiate Athletic Association (NCAA) Division I college wrestlers during and after a season. Methods: Ohio State University wrestling team members (N = 6; mean [SD] age = 19.6 [1.1] y; height = 171.6 [2.9] cm; body mass = 69.5 [8.1] kg) gave informed consent to participate in the investigation with measurements (ie, body mass, urine-specific gravity [USG; 2 methods], Visual Analog Scale thirst scale, plasma osmolality) obtained during and after the season. Results: Measurements for USG, regardless of methods, were not significantly different between visits, but plasma osmolality was significantly (P = .001) higher at the beginning of the season—295.5 (4.9) mOsm·kg−1 compared with 279.6 (6.1) mOsm·kg−1 after the season. No changes in thirst ratings were observed, and the 2 measures of USG were highly correlated (r \u3e .9, P = .000) at each time point, but USG and plasma osmolality were not related. Conclusions: A paradox in the clinical interpretation of euhydration in the beginning of the season was observed with the USG, indicating that the wrestlers were properly hydrated, while the plasma osmolality showed they were not. Thus, the tracking of hydration status during the season is a concern when using only NCAA policies and procedures. The wrestlers did return to normal euhydration levels after the season on both biomarkers, which is remarkable, as previous studies have indicated that this may not happen because of the reregulation of the osmol-regulatory center in the brain

The betaine content of sweat from adolescent females

Abstract Background This study was developed to establish whether betaine was present in the sweat of females and to determine any correlations with other sweat components. Methods Sweat patches were placed on eight trained adolescent Highland dancers (age = 13.6 ± 2.3 yr), who then participated in a dance class for 2 hours. Patches were removed, and the sweat recovered via centrifugation. The sweat was subsequently analyzed for betaine, choline, sodium, potassium, chloride, lactate, glucose, urea and ammonia. Results Betaine was present in the sweat of all subjects (232 ± 84 μmol·L-1), which is higher than typically found in plasma. The concentration of several sweat components were correlated, in particular betaine with most other measured components. Conclusion Betaine, an osmoprotectant and methyl donor, is a component of sweat that may be lost from the body in significant amounts

Relationships of Physical Performance Tests to Military-relevant Tasks in Women

Purpose: This investigation sought to determine the most predictive measures of performance on a repetitive box lifting task (RBLT) and load bearing task (LBT) among 123 women (aged 23±4 years, height 165±7 cm, body mass 64±10 kg). Methods: To determine the relationship of various predictors to performance on the RBLT and LBT, multiple regression analysis was conducted on body mass, height, leg cross-sectional area, upper and lower body muscular strength, lower body explosive power, upper and lower body local muscular endurance, and aerobic capacity. Results: The mean±SD (range) number of repetitions for the RBLT was 86±23 (20-159). The mean±SD (range) time to complete the LBT was 2,054±340 seconds (1,307-3,447). The following equations were generated: RBLT (number of repetitions)=57.4 + 0.2(peak jump power) + 0.4(number of pushups in 2 minutes) + 0.15(number of repetitions during the squat endurance test) + 1.39(one repetition maximal strength boxlift (kg)) – 0.04(2-mile run time (2MR) in seconds), R=0.81; standard error of the estimate (SEE)=14; LBT (in seconds)=1,831 – 4.28(number of repetitions during the squat endurance test) + 0.95(2MR in seconds) – 13.4(body mass), R=0.73; SEE=232. Conclusions: We found that the 2MR and squat endurance test were signifi cant predictive factors for performance on both load carriage tasks. These data also imply that women’s performance in combat-related tasks can be improved with training that targets muscular strength, power, and local muscular endurance in addition to aerobic capacity

Unos L-alanil-L-glutamina tijekom kratkotrajne visokointenzivne vježbe u uvjetima blagoga hidracijskoga stresa

The effect of acute L-alanyl-L-glutamine (AG) ingestion on selected hormonal and electrolyte measures was examined during repetitive, short duration, high intensity exercise with mild hypohydration. Subjects (20.3±1.1 yrs; 180.3±10.4 cm; 83.1±14.0 kg; 11.6±3.6% body fat) reported to the Human Performance Laboratory on four occasions. During each trial subjects were hypohydrated to -2.5% of their baseline body mass. During one trial (DHY) subjects rested in a recumbent position for 45 minutes before commencing the exercise session. During the other three trials subjects were rehydrated to 1.5% of their baseline body mass, before exercise, by drinking water only (W), or with two different doses of AG – a low dose (LDAG: 0.05 g�kg-1) and a high dose (HDAG: 0.2 g�kg-1). The exercise protocol consisted of ten 10-second sprints on a cycle ergometer with a 1-min rest between each sprint. Blood draws were collected once the subject achieved the desired level of hypohydration, immediately pre-exercise, immediately post-exercise, and 24 hrs postexercise. Blood samples were analyzed for glutamine, potassium, sodium, aldosterone, arginine vasopressin, C-reactive protein, interleukin-6, malondialdehyde, testosterone, cortisol, ACTH, and growth hormone. The area under the curve (AUC) analysis demonstrated significantly greater sodium concentrations for DHY compared to all other trials. The AUC analysis for aldosterone showed significantly lower concentrations at LDAG compared to DHY. No other differences between trials were observed in any other hormonal or biochemical responses. AG ingestion during a short duration, anaerobic exercise and mild hypohydration stress had a limited effect on selected hormonal and biochemical measures.Učinci akutnoga, trenutačnoga uzimanja L-alanil-L-glutamina (AG) na odabrane hormonske i elektrolitne pokazatelje ispitani su tijekom ponavljajuće kratkotrajne visokointenzivne aktivnosti u uvjetima blage hipohidracije ispitanika. Ispitanici (20,3±1,1 godina; 180,3±10,4 cm; 83,1±14,0 kg; 11,6±3,6% tjelesne masti) bili su testirani u Human Performance Laboratory u četiri navrata. Tijekom svakoga pojedinačnoga mjerenja ispitanici su bili hipohidrirani do -2,5% svoje početne, osnovne tjelesne mase. Tijekom prvoga testiranja (DHY) ispitanici su se odmarali ležeći 45 minuta prije no što su počeli provoditi protokol vježbanja. Tijekom sljedeća tri mjerenja ispitanici su rehidrirani do 1,5% njihove početne tjelesne mase prije vježbanja, i to: pijenjem samo vode (W) te unosom dviju različitih doza AG - male doze (LDAG: 0,05 g∙kg-1) i velike doze (HDAG: 0,2 g∙kg-1). Protokol vježbanja sastojao se od po deset sprintova na bicikl-ergometru u trajanju od 10 sekunda s jednominutnim odmorom između svakoga sprinta. Uzorci krvi vađeni su odmah nakon što je ispitanik dosegao željenu razinu hipohidracije, neposredno prije početka vježbanja, neposredno nakon završetka vježbanja i 24 sata nakon vježbanja. U uzorcima krvi analizirana je koncentracija glutamina, kalija, natrija, aldosterona, arginin vazopresina, C-reaktivnoga proteina, interleukina-6, malondialdehida, testosterona, kortizola, ACTH-a i hormona rasta. Analiza površine ispod krivulje pokazala je statistički značajno veću razinu koncentracije natrija u ispitanika u prvom testu (DHY) u odnosu na sva ostala mjerenja. Analiza površine ispod krivulje za aldosteron je pokazala značajno nižu koncentraciju u testu LDAG u odnosu na test DHY. Nisu zapažene značajne razlike između pojedinih mjerenja ni u jednoj drugoj hormonskoj i biokemijskoj reakciji na protokol vježbanja. Uzimanje AG tijekom kratkotrajne anaerobne aktivnosti i u stanju blagoga hipohidracijskoga stresa pokazalo je ograničene učinke na odabrane hormonske i biokemijske pokazatelje