2 research outputs found
Comparison of endometrial biopsy and postoperative hysterectomy specimen findings in patients with atypical endometrial hyperplasia and endometrial cancer
Objectives: The aim of the study was to assess the concordance between the preoperative endometrial sampling and microscopic examination of the hysterectomy specimens in patients surgically treated for atypical endometrial hyperplasia and endometrial carcinoma.
Material and methods: We analysed a group of 204 patients, of whom 160 (78.43%) underwent surgical treatment for cancer of the corpus uteri and 44 (21.57%) for atypical endometrial hyperplasia. The preoperative diagnosis was based on the histological examination of endocervical and endometrial samples obtained by fractional curettage and it was compared to the histological findings at hysterectomy. The comparison was made for the basic diagnosis, the histological type of the cancer and the grade of tumour differentiation.
Results: When the histological types of cancer diagnosed in endometrial curettage and hysterectomy specimens were comÂpared, the concordance was observed in 134/160 patients (83.75%). The highest concordance was found for endometrioid carcinoma (127/148 patients, 85.81%). The grade of tumour differentiation was accurate in 69.31% of patients. The highest concordance was for moderately differentiated carcinomas. Of 44 patients who underwent surgical treatment for atypical endometrial hyperplasia, the preoperative diagnosis was confirmed by the postoperative histopathological examination in 21 patients (47.73%). In 15 patients (34.09%) endometrial cancer was diagnosed at hysterectomy.
Conclusions: In endometrial cancer our findings demonstrate a high level of concordance between the histological diagnosis on endometrial curettage and at hysterectomy. Own observations have confirmed that over 30% of patients undergoing surgical treatment for atypical endometrial hyperplasia have concurrent endometrial cancer which is determined by surgery
The expression of MMP-14 and microRNA-410 in FFPE tissues of human endometrial adenocarcinoma
Endometrial cancer (EC) is the most
common gynecological malignancy in Europe and North
America. It is classified into two types exhibiting
different characteristics and prognosis. Type I is an
estrogen-dependent tumor, histologically classified as
low grade and low stage, usually with an excellent
prognosis. Type II EC is unrelated to estrogen
stimulation and is characterized by a poor prognosis.
MicroRNAs (miRNAs, miRs) are small non-coding
RNA polynucleotides that regulate gene expression posttranscriptionally. Various dysregulations in microRNA
expression are often considered to have an impact on the
diagnosis, prognosis and overall survival in patients
diagnosed with different types of cancers. Recent data
suggest that microRNAs play an important role in the
pathogenesis of EC.
The aim of the study was to evaluate the
involvement of matrix metaloprotease 14 (MMP-14) and
microRNA-410 in formation of the EC tumor. To this
end expression of MMP-14 and microRNA-410 was
assessed within the cancer, transient and healthy zones in
the histological sections of tumours using immunohistochemical staining and laser capture microdissection
(LCM) followed by a quantitative real-time PCR. The
results revealed significantly higher expression of MMP14 in the cancer tissue zone in comparison to the healthy
tissue zone, as well as a lower expression of microRNA410 in the cancer zone compared with the healthy zone.
This reverse correlation may suggest a regulatory role of
miRNA-410 in modulating levels of MMP-14 in EC.
This is the first report on such regulation in human
endometrial cancer